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On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数,结果表明:①酶-底物二元、三元复合物的失活速度明显慢于游离酶,说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用;②在脲作用下,酶的失活和构象变化均为单指数项过程,而且酶的活力丧失和三级结构变化是同时发生的,说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程;③在盐酸胍作用下,酶的构象变化为两相过程,而失活则是单指数项过程,酶分子构象变化的快相速度常数与失活速度常数基本一致,因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。

Nothing could extinguish his faith in human nature.

没有什么能使他丧失对人性的信心。

Nothing could extinguish our faith in human nature.

没有什么能使我们丧失对人性的信心。

The basic intension of this course,shown as and developed what is useful and discard what is not the traditional legal system,on the basis of attracting the western legal theory and legal principle,set up the new legal system with modern significance The forfeiture of the extraterritoriality,not only has destroyed the judicial sovereignty of China seriously,and the national pride and self-confidence with Chinese serious injury.

这一过程的基本内涵,表现为扬弃传统法律体系,在吸收西方法律理论和法律原则的基础上,建立具有近代意义的新的法律体系。治外法权的丧失,不但严重破坏了中国的司法主权,而且也严重损害了中国人的民族自尊心和自信心。

The basic intension of this course, shown as and developed what is useful and discard what is not the traditional legal system, on the basis of attracting the western legal theory and legal principle, set up the new legal system with modern significanceThe forfeiture of the extraterritoriality, not only has destroyed the judicial sovereignty of China seriously, and the national pride and self-confidence with Chinese serious injury.

这一过程的基本内涵,表现为扬弃传统法律体系,在吸收西方法律理论和法律原则的基础上,建立具有近代意义的新的法律体系。治外法权的丧失,不但严重破坏了中国的司法主权,而且也严重损害了中国人的民族自尊心和自信心。

After the Opium War, with the loss of the extraterritoriality, the development of the international prison-reforming movement and the collision and fusion of the Chinese and Western law thoughts, the modern prison-reforming thoughts that took the bourgeois reformists as the representatives came into being.

鸦片战争后,随着治外法权的丧失、国际监狱改良运动的发展以及中西法律思想的碰撞融合,以资产阶级改良派为代表的近代监狱改良思想初步形成。

Because of the loss of the extraterritoriality and tariff autonomy,the collection of stamp tax presented a serious phenomenon:it could not popularize to the foreign businessmen and even the Chinese businessmen lived in the foreign settlement.

由于治外法权和关税自主权的丧失,使得印花税的征收出现了严重的内重外轻现象,税法不能普及洋商乃至租界华商,增加了印花税法推广的难度,而这一问题,至北京政府结束,中外磋商仍未完成,留给国民政府一个急待解决的难题。

These factious groups are more violent than their predecessors and their fanaticism is even more irrational.

这些宗教派组织比他们的前辈更残暴,他们的狂热变得越发丧失理性了。

I've lost faith in your ability.

我对你的能力丧失了信心。

I could almost bear the falseness and the lies, but I could not forgive its complete absence of humor.

我几乎可以忍受它的谎言,它所犯下的错误,然而我不能原谅它彻底丧失了幽默感。

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推荐网络例句

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了,但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场,你等着挨绳子吧!

Several theories about reigniting and extinguishing of the arc have been refered.

本文综合考虑了几种电弧重燃和熄灭理论。