两色的

Instead of calling on some scholar, I paid many a visit to particular trees, of kinds which are rare in this neighborhood, standing far away in the middle of some pasture, or in the depths of a wood or swamp, or on a hilltop; such as the black birch, of which we have some handsome specimens two feet in diameter; its cousin, the yellow birch, with its loose golden vest, perfumed like the first; the beech, which has so neat a bole and beautifully lichen-painted, perfect in all its details, of which, excepting scattered specimens, I know but one small grove of sizable trees left in the township, supposed by some to have been planted by the pigeons that were once baited with beechnuts near by; it is worth the while to see the silver grain sparkle when you split this wood; the bass; the hornbeam; the Celtis occidentalis, or false elm, of which we have but one well-grown;some taller mast of a pine, a shingle tree, or a more perfect hemlock than usual, standing like a pagoda in the midst of the woods; and many others I could mention.

我并没有去访问哪个学者，我访问了一棵棵树，访问了在附近一带也是稀有的林木，它们或远远地耸立在牧场的中央，或长在森林、沼泽的深处，或在小山的顶上；譬如黑桦木，我就看到一些好标本，直径有两英尺：还有它们的表亲黄桦木，宽弛地穿着金袍，像前述的那种一样地散发香味，又如山毛榉，有这样清洁的树干，美丽地绘着苔藓之色，处处美妙呵，除了一些散在各地的样本，在这乡镇一带，我只知道有一个这样的小小的林子，树身已相当大了，据说还是一些被附近山毛榉的果实吸引来的鸽子播下的种子；当你劈开树木的时候，银色的细粒网闪发光，真值得鉴赏；还有，椴树，角树；还有学名为Celtis occidenta lis的假榆树，那就只有一棵是长得好的；还有，可以作挺拔的桅杆用的高高的松树，以及作木瓦用的树；还有比一般松树更美妙的我们的铁杉，像一座宝塔一样矗立在森林中；还有我能提出的许多别的树。

Furthermore, out of 497 fAFLP markers, 80 special bands were found to be able to distinguish the four groups from each other and may be applied for germplasm characterization and molecular assistant classification of Meretrix clam.4 Molecular classification of two species of Meretrix clam based on fAFLP and ITS sequences4.1 The results of fAFLP maker analysis of S, G and W showed that each group had their own specific loci among which there were 53 special loci in W group, much more than those of S group (14) and G group (21). Among the 53 loci, nine were all dominant loci. These unique loci could be taken as molecular markers to distinguish W from other groups. The genetic similarity indexes and distance matrix between S and G groups were 0.9585 and 0.0424 respectively, but the genetic similarity indexes and distance matrix between W group and S or G group was 0.7939 or 0.7941, and 0.2308 or 0.2305 respectively. The results revealed that significant difference existed between W and S or G groups in molecular genetic structure. The phylogenetic trees by the methods of UPGMA and NJ also indicated that S and G populations were very closely related, while W population was a relatively independent cluster, lying beyond the species which S and G belong to.4.2 The internal transcribed spacer region of the rDNA from S group, G group and W group were PCR amplified and sequenced. The results showed that the size of ITS ranged between 1266-1269bp in W group, while those in G and S groups were 1614bp and 1520bp respectively. The GC content ranged 62.32-62.62% in W group while it was 61.77% in G group. The genetic distances between three populations of W group were 0.001～0.003, but it was 0.110 or 0.147 respectively between W group and G group or S group. Phylogenetic trees by NJ method also showed that G group was very closely related to S group, while W group was a relatively independent cluster.

在457个总扩增位点中找出了53个W的特有位点，远多于S群体(14)和G(21)群体，而且在53个特有位点中有9个出现频率为100%的位点，这些位点可以作为区分其它2个群体的特征性标记；S– G群体特有的位点有112个，其中有4个位点出现频率为100%，可作为S– G群体区别于W群体的特征性标记。S群体和G群体间的遗传相似性系数为0.9585，遗传距离只有0.0424，在NJ和UPGMA法构建的亲缘关系的树状图上均首先聚在一起，说明二者的亲缘关系很近，应属于种内群体间的关系；而W与S和G的遗传相似性系数均较小(0.7939和0.7941)，相对遗传距离很大而且十分相近(0.2308和0.2305)，在亲缘关系树状图上单独分出一支，也表明W与S和G群体间的亲缘关系较远。4.2 ITS序列比较分析通过对白壳文蛤、山东文蛤和广西文蛤的ITS序列扩增电泳、PCR-RFLP分析和ITS序列分析发现，W的ITS序列长度在1266-1269 bp，而S和与G的ITS序列总长度分别为1520 bp和1614 bp；从ITS1和ITS2长度来看，W分别为739-741 bp和316-317 bp，S为895 bp和414 bp，G为987 bp和416 bp；而从ITS碱基组成来看，W的GC含量在62.32-62.62%之间，而G群体为61.77%。W的3个壳色不同群体间的遗传距离仅0.001、0.002和0.003，S与G群体间的遗传距离是0.010，说明W群体内变异很小，而S与G群体间已出现明显的遗传分化，但还均属于种内群体间的遗传变异；而W与G和S的遗传距离分别达到0.110、0.147，两个类群差异显著，已远超出种内群体间的遗传变异。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。