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The molecular weight of broad bean vicilin and legumin measured by gel filtration chromatography were 135000 and 351000, DSC showed that their denature temperatures were 72℃ and 87℃ respectively. The legumin had a lowest solubility at pH 4. 5-5. 0 whereas the vicilin at pH5. 5. Vicilin and legumin were all possessed low levels of α-helical and high β-sheet secondary structure fractions. The total content of α-helical,β-sheet and β-turn fraction of vicilin and legumin reached to 90% and 78% separately showed that they had a high ordered structure in solution.

对两种组分的凝胶层析表明7S和11S的相对分子质量分别为135000和351000;DSC分析显示其热变性温度分别为72℃和87℃;溶解度分析表明:11S球蛋白在pH4.5-5.0之间,7S球蛋白在pH5.5时具有最低的溶解度;圆二色性分析结果显示7S和11S球蛋白的β-折叠结构含量分别高达40.5%和38.1%,证明二者都属于β-折叠型蛋白质,二者的α-螺旋、β-折叠和β-转角结构的含量总和分别达到了90%和78%,从而说明它们在溶液中的结构是高度有序的。

In every group, rabbits were subdivided into experimental and control subgroups. 2 Rabbits were bullets injected followed by continuous injected with 13C labeled leucine, glucose, and lactic acid; 3 Blood were drewed before and 150, 160, 170, and 180 min after the initiation of isotopes injection for material analysis; 4 Exhale gas were collected every 5 min in the first 30 min followed by every 30 min there after for material analysis; 5 After centrifuge in low temp the supernatant of the blood samples were collected and went through axon and anon exchange column treatment; 6 Treated blood sample was used for 13C labeled leucine, glucose lactic acid examination through mass spectrograph; 7 The exhale gas was collected for 13CO2 exam through gas-phase mass spectrograph; 8 CO2 total production rate (V13CO2), body various substances production, oxidation speed, and substances metabolic percentage all can be calculated through equations provided by references below; 9 Data was processed through student t test.

分亮氨酸、葡萄糖和乳酸三组各取健康新西兰兔16只,每组再分为高代谢脓毒症组和对照组两部分,建模方法同第一部分;2)三组分别先静脉弹丸式推注再持续灌注13C标记的亮氨酸、葡萄糖和乳酸;3)灌注前抽取动脉血作为背景值,灌注达150,160,170和180min分别抽取动脉血2ml用于质谱分析;4)实验开始后前30min中每5min收集呼出气一次,随后每30min收集呼出气一次,用于气相质谱分析;5)血样经过低温离心取上清液,过阳离子及阴离子交换树脂,再经衍生化处理;6)处理过的血样进入气相色谱-质谱仪,测量其13C标记的亮氨酸、葡萄糖和乳酸丰度;7)实验兔的呼出气通过13CO2气相质谱分析仪测定其中的13CO2丰度(E13CO2);8)利用文献提供的公式算出CO2总生成率(V13CO2)以及机体各物质的通量、氧化速率以及物质代谢百分比等;9)数据分析处理采用t检验。

Heat-treating within the temperatures range 700 to 800℃, the transition temperature of heat-treated C-glass became higher with increasing heat-treatment temperatures and gradually approached the transition temperature of the B-glass without TiO〓; the appearance of the C-glass gradually changed from brown-violet colour to intense blue-violet colour and even to opalescence; and the phase separation morphology of TiO〓-rich phase gradually changed from an interconnected phase to a siliceous droplet phase embedded in SiO〓-rach matrix phase with increasing the heat-treatment temperatures.

在玻璃的转变点附近的温度范围内热处理含TiO〓系统玻璃时,随热处理温度的升高,玻璃的转变温度增大,逐渐向不含TiO〓的MgO-Al〓O〓-SiO〓系统玻璃的转变温度靠近;玻璃的颜色逐渐由紫色向兰色及乳白色转化,这是分相玻璃中两相组成在热处理过程中的变化所引起的。

Suited to the style: the whole bottle is good for the eyes bright look for more hair, long hair and short hair can be, natural color and good, but due to reasons like the popular, relatively dark hair dyed the color of Daoshi also be considered ; if long hair, do not Pisan with, it is best comb generated a high number of exquisite feeling; older women may be smaller bottles comb two high pigtails, age, bigger, then comb a Mawei on it.

适合的样式:总体来说瓶子适合比较看起来能让人眼前一亮的发型,长发短发都可以,自然色不错,但是由于喜欢流行的原因,把头发染成比较深的颜色倒是也可以考虑;若是长发,不要披散着,最好是梳的高一些产生玲珑的感觉;年纪小一点的瓶子女可以梳两个高辫子,年龄大些的话,梳个马尾就可以了。

Using fuji apple seed and new red star apple seed as the material,focuses on determination of the content of crud protein and crude fat,and analysis of physical-chemical properties and fatty acid composition in their oil.

以富士和新红星两个品种的苹果籽为原料,经索氏提取后测定了它们的粗蛋白含量、粗脂肪含量及理化性质,用气相色谱法分析了其脂肪酸组成,结果表明,富士和新红星苹果籽的粗蛋白含量分别为48.85%和49.55%,粗脂肪含量分别为23.69%和24.32%;苹果籽油的理化指标符合食用油标准,富士苹果籽油中油酸和亚油酸的含量分别为37.49%和51.40%,新红星苹果籽油中油酸和亚油酸的含量分别为38.55%和50.70%,苹果籽油具有良好的商业开发前景。

This thesis includes five parts as follows.1. The backgrounds and some relative concepts of this work are briefly introduced. The photochemistry of benzamides and compounds with thiourea functional group is also concisely reviewed.2. Total of fourteen benzamides with different substituted groups have been synthesized and structurally characterized. Better photoreaction time for that kind of compounds at given concentrations is confirmed by tracking its'UV spectrum and GC chromatogram. A representative dibenzophenone photoproduct has been determined by X-ray crystallography. The mechanisms of photoreaction of benzamide have been proposed.3. Total of seven N-chloro(phenylmethylenebenzenamines with different substituted groups and sixty-one thiourea derivatives have been synthesized and structurally characterized.

论文的主要内容如下:1、简要介绍本论文工作的研究背景,并对苯甲酰胺和具有硫脲官能团类化合物的光化学研究进展进行简要的总结。2、合成与表征了14个具有不同取代基的苯甲酰胺类化合物,利用紫外光谱和气相色谱确定了此类化合物较佳光照时间,测定了一个具有代表性光解产物的单晶结构,提出了苯甲酰胺类化合物的光反应机理。3、合成与表征了7个带有不同取代基的氯代苯甲酰亚胺中间体及61个硫脲衍生物,测定了两个具有代表性化合物的单晶结构。

Furthermore, the brain area of anterior cingulated cortex revealed different response type under emotional and non-emotional interference condition, i.e, activation of ACC in color-word Stroop task was enhanced along with increasing of cognitive conflicting interference. Yet in emotional Stroop task, activation of ACC is reduced when the negative emotional interference was enhanced. This phenomenon suggests that ACC possess a flexible conflict monitoring mechanism and adaptive mechanism, thus providing evidence for the theory that ACC plays an important role in the cognitive and emotional interaction.

其结果显示,背侧前额叶、前扣带回是共同参与情绪与非情绪干扰的执行抑制的脑区,其中,前扣带回在情绪与非情绪干扰两种条件下出现不同类型的反应方式,即前扣带回在色字Stroop作业中随认知冲突干扰梯度的增加而增加,但在情绪Stroop作业中随负性情绪干扰梯度的增加而减少,体现出前扣带回具有灵活的冲突监控与适应性机能,支持前扣带回在认知与情绪交互作用中扮演重要角色的理论。

The molar ratio of sulfate ions to carboxylate ions is determined by conductometric titration method and the molecular weight is tested by high performance gel permeation chromatography. The precision of these two methods is also studied. To improve the detection sensitivity, guanidine hydrochloride is used as the best derivation reagent of 911. A systematic research is done on the optimum derivatization condition of 911 and Guanidine hydrochloride. The M /G ratio of 911 has direct relation to its biological activity.

论文首先对911的基本理化性质进行了系统的分析,以电导滴定法和高效凝胶渗透色谱法分别测定了分子中硫酸根羧酸根的比值和911的分子量,并探讨了两种分析方法的精密度。911分子没有紫外吸收,为提高检测灵敏度,本文采用荧光标记法,通过对三种荧光试剂与几种寡糖和多糖衍生效果的综合比较,最终确定了盐酸胍为最佳衍生试剂,并以911为代表详细探讨了衍生化反应的最优条件。911糖链结构与褐藻胶主链结构类似,以甘露糖醛酸和古罗糖醛酸的嵌段形式存在,分子中M/G比值与911的物理化学性质和生物活性有直接关系。

The methods used for collecting samples and establishing specific HAB cultures are described in detail. Methods of SEM and ESEM (environmental scanning electron microscope) for Gymnodinium were developed an...

建立了赤潮监测中样品采集处理、分离和纯化培养的技术体系;建立和优化了裸甲藻扫描电镜观察技术和环境样品的扫描电镜观察方法;提出了库尔特计数是相对简便、快捷、准确、可行的细胞计数方法;应用基于反相高效液相色谱的光合色素分析技术及原位荧光激发光谱测定方法,得出不同类群浮游植物对不同的营养盐限制存在不同响应机制的结论;用荧光强度来监测浮游植物生理状态及营养状态变化具有一定的可行性,HPLC和原位荧光两种方法指示浮游植物的生理状态存在一定差异。2。

The creative point of this dissertation consists firstly in that monochromatic stimuli instead of combined spectral power distribution were used as visual object, and spectral luminous efficacy curves were drawn from the performance-based method; Comparison between performance-based method and brightness-matching method was then done and therefore connection between these two methods was developed; One more creative point is that the analysis based on the theory of neurophysiology and psychophysics leads to the conclusion that contribution of the colour channel during visual information processing is reflected from performance-based method, which should be considered when CIE develops the new mesopic photometry system.

论文的新颖之处在于实验中使用了单色光谱代替复合谱或物体色作为视觉目标,在分析中间视觉状态下视觉功能的光谱响应特性的基础上反推了人眼的光谱光视效率函数;论文更进一步验证了基于视觉功能法和传统的视亮度匹配法之间的联系,在两种方法之间构造了一座桥梁,为基于视觉功能法的使用初步建立了理论依据;同时论文创新地采用心理物理学的方法进行实验和结合物理学和视神经生理学的知识对实验结果进行分析,从而从视觉信息处理的神经机制层面证明了基于视觉功能法获得的结果包含了视觉颜色通道效应,并对CIE在建立新的中间视觉光度学系统时要充分考虑这一视觉颜色通道的影响提出了建议。

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