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To improve detection efficiency and result accuracy, we developed an improved multiplex PCR technique for specific detection of GMO.①The assay strategy involves multiplex PCR amplification of the target with several biotinylated primer pairs in one tube, followed by the hybridization of multiplex PCR products and membrane-bound oligonucleotide probes. The multiplex PCR/probe hybrid is detected colorimetrically using an alkaline phosphatase conjugate and a chromogenic substrate.

在多基因定性检测技术方面,本文在优化并确定了多基因PCR反应条件的基础上,采用了两种方法对多基因PCR产物进行鉴定:①采用共价结合方法将多个探针固定在Biodyne〓C膜上,将5'端标记生物素的多基因PCR产物与膜上的探针进行杂交及显色反应,首次建立了GMO的多基因PCR-膜相杂交检测方法,实现了一个反应体系中同时检测多种转基因成分的目标,提高了检测效率与准确率。

The molar ratio of sulfate ions to carboxylate ions is determined by conductometric titration method and the molecular weight is tested by high performance gel permeation chromatography. The precision of these two methods is also studied. To improve the detection sensitivity, guanidine hydrochloride is used as the best derivation reagent of 911. A systematic research is done on the optimum derivatization condition of 911 and Guanidine hydrochloride. The M /G ratio of 911 has direct relation to its biological activity.

论文首先对911的基本理化性质进行了系统的分析,以电导滴定法和高效凝胶渗透色谱法分别测定了分子中硫酸根羧酸根的比值和911的分子量,并探讨了两种分析方法的精密度。911分子没有紫外吸收,为提高检测灵敏度,本文采用荧光标记法,通过对三种荧光试剂与几种寡糖和多糖衍生效果的综合比较,最终确定了盐酸胍为最佳衍生试剂,并以911为代表详细探讨了衍生化反应的最优条件。911糖链结构与褐藻胶主链结构类似,以甘露糖醛酸和古罗糖醛酸的嵌段形式存在,分子中M/G比值与911的物理化学性质和生物活性有直接关系。

The total ion chromatograms of cuticular extracts were different markedly and can be used to distinguish the two species insects.

两种害虫的提取物的总离子流色谱图具有明显差异,可以用于区别米象和玉米象。

The as-grown sapphire boule (φ110×80mm3) turns out colorless and transparent after being decolourized and decarbonized in air and hydrogen successively at high temperature.

将TGT法生长的Al2O3晶体(φ110×80mm3)依次经过高温氧化气氛、高温还原气氛脱碳、去色退火实验,即&两步法&退火实验,晶体变成无色、透明。

Its test result, after using agent of sewage disposal of biology of carapace god ocean and technology of flood body rehabilitate, build good oxygen biology to stabilize a bed, processing sewage effect is apparent, expression is: Black smelly sewage is rapid decolour, deodorization, become clear, insipidity, the COD after handling two days by 333 drop 76, achieve view and admire water and the water quality demand that breed water, can develop aquatic substance. A green alga, red snapper grows to feed after 4 days wait for primary live thing, breed the water biology such as water hyacinth to be able to put in a suitable place to breed view and admire a fish.

其试验结果,运用甲壳神海洋生物污水处理(通过物化方法去除水中一些物质的过程)剂和大水体修复技术后,建立好氧生物稳定床,处理污水效果明显,表现为:黑臭的污水迅速脱色、脱臭,变得澄清、无味,处理两天后COD由333下降到76,达到观赏水和养殖水的水质要求,可培养水生物。4天后长出绿藻、红鱼食等初级生物,养殖水葫芦等水生物并可放养观赏鱼。

So I can found the elementary i- dentification system for characteristic fingerprints the fingerprints.

而且实验中所得到的气相色谱特征指纹图谱的标准为这两种香精香料质量的控制提供了理论依据。

Cut them out with the pen tool and desaturate them (Command + Shift + U).

打开两个铆钉图片,用钢笔工具抠出铆钉,去色(Ctrl+Shift+U)。

Independently Selectable Voicings for each harmony, with a wide variety of options, including two intelligent shifting modes, unison/detune, octave up/down, straight chromatic shifts from -12 to +12 semitones, as well as 2 octaves down.

独立可选择voicings为每和Xie,与各种各样的选择,其中包括两个智能转移模式,异口同声地/的失谐,八度向上/向下,直色的转变,从-12到12半音,以及二八度音。

Independently Selectable Voicings for each harmony, with a wide variety of options, including two intelligent shifting modes, unison/detune, octave up/down, straight chromatic shifts from -12 to +12 semitones, as well as 2 octaves down.

72EC'7ACB 可选择voicings为每和Xie,与各种各样的选择,其中包括两个智能转移模式,异口同声地/的失谐,八度向上/向下,直色的转变,从-12到12半音,以及二八度音。

Adding dividedly the solution of two drugs with different concentration into the heperplastic scar fibroblasts and keloid fibroblast which were in logarithmic growth phase. After 48 hours in vitro culture, hydroxyproline colorimetric test was conducted, then statistics comparison was carried out in accordance with hydroxyproline content which was calculated based on OD value.

向对数生长期的增生性瘢痕成纤维细胞及瘢痕疙瘩成纤维细胞中加入不同浓度的两种药液,培养48小时后进行羟脯氨酸比色试验,并将 OD 值依公式换算成羟脯氨酸含量,进行统计学比较。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。