两细胞的

Src l was an acidic cytolysin, which was reported forthe first time. The cDNA encoding the mature Src l was cloned into non-fusion expression vectorpBV220 and expressed successfully in E.coli DH5α in inclusion body. After washing anddenaturing-renaturing, the recombinant Src l protein was purified by Q Sepharose FastFlow ion exchange chromatograph and Phenyl Sepharose hydrophobic interactionchromatograph.

首先构建了Src I基因的非融合蛋白表达质粒pBV220-Src I，并成功地在原核细胞E.coli进行了重组表达，筛选出稳定表达的工程菌株DH5α，摸索了稳定表达的条件，摸索了包涵体的洗涤、变性和复性条件，摸索出了复性蛋白Src I的纯化工艺，纯化过程简单，经QSepharose Fast Flow阴离子交换层析和Phenyl Sepharose疏水层析两步纯化，就可获得了高纯度的重组蛋白样品(纯度达99％以上)，该纯化方法适合重组蛋白的大量纯化，为Src I的性质、结构和功能研究奠定了良好的基础。

The result indicates the t-BHP or diamide both cause the mass loss about the membrane protein thiol in hepatocytes, the fast oxidization about glutathione in hepatocytes, the leakage of lactate dehydrodenase, but the level of t-BHP is larger than of diamide. In the aspect of lipid peroxidation, only the t-BHP produces apparent change. As to actin and tubulin, they both find fall-off and present time-dependency effects. The result in Confocal fluorescent microscope also show these two cytoskeletal proteins are related to cell blebbing, but they don''t play the same role during the porcess. Tubulin may be more important than actin. If the study predispose dithiothreitol before adding t-BHP or diamide, then the damage by the diamide is repressed, but just partly protection for the damage by t-BHP.

结果显示t-BHP或diamide均可造成细胞膜蛋白硫醇大量流失与胞内GSH快速氧化，亦显著导致乳酸去氢□渗漏，但t-BHP诱发的渗漏程度大於diamide；在脂质过氧化方面，则仅t-BHP处理组产生明显的脂质氧化情形；至於肌动蛋白与管蛋白单体均可见减少且呈现时间依赖效应；在雷射共轭焦萤光显微镜观察中，亦显现这两种架构蛋白与细胞膜起泡有关，但两者在小泡生成的过程中扮演的角色并不完成相同，可能管蛋白较肌动蛋白重要；如果细胞在t-BHP或diamide加入前预先以dithiothreitol处理，则diamide 所造成的伤害显著的受到抑制，但DTT对t-BHP所诱发的伤害则仅有部分保护效果。

In blastula embryos and 50% epiboly embryos, the zygotic pou2 transcript is also distributed in every blastomere cell. At the stage of 90% epiboly, expression of pou2 converges to the dorsal midline of embryos and locates in two waves of lateral neural plate and two transverse stripes in the brain region. In embryos at 100% epiboly, pou2 transcript is restricted in the neural plate and areas of presumptive midbrain amid himdbrain. From somite stage, gibel carp pou2 can not be detected any more, which is consistent with the RT-PCR result.

在高囊胚期和50%下包期，高度表达的合子转录本仍在所有的胚盘细胞中，但至90%下包期时，pou2的表达向胚胎背部的正中线汇聚，集中在神经板的两侧区域和脑部的两条横向条带在100%下包期时，pou2的表达集中在神经板的中间区域以及预期形成的中后脑区域至体节期时，转录本消失，这与RT-PCR结果高度一致。

The results showed that there were two kinds of grains in the cells,one was large and had low electron density,Gl for short,while the other was small and had high electron density,Gh for short.The latter ones had a circle around it which can be seen through the microscope.During the aroma releasing period,the shape of the two grains changed distinctly,which led to the inference that Gl was the leucoplast,the store-room of aroma precursors or energe substances,and that the Gh was probably the lysosome which is related to the enzyme system?s aroma producing.The circle around Gh is the formed fragrant constituents and the aroma forming is the result of the interaction of Gh and Gl .

结果发现，茉莉花瓣细胞中存在电子密度较低、体积较大的颗粒和电子密度较高、体积较小的颗粒（分别简称为Gl和Gh颗粒），并在Gh颗粒周围存在清晰的晕圈；在茉莉花释香过程中，两种颗粒在形态上均发生明显的变化，推测Gl颗粒可能是白色体，是茉莉花香气前体物或能量物质的贮存器，Gh颗粒可能为溶酶体，与香气形成的酶系统有关，Gh颗粒周围存在的晕圈可能是香气物质，香气物质的形成是Gl和Gh颗粒相互作用的结果。

Thus, salicylate is widely used for studying the mechanisms of tinnitus. The aim of the present study was to investigate the effects of salicylate on voltage-gated sodium, potassium and calcium channels in the inferior colliculus and the auditory cortex neurons by use of the whole-cell patch clamp method, in order to obtain insight into the relations of tinnitus and voltage-gated channels.

本研究首次将膜片钳的实验技术和离子通道的基础理论引入耳鸣中枢机制的研究中，利用全细胞膜片钳记录技术，观察水杨酸钠对大鼠听觉通路上的两个重要部位——下丘、颞皮层的神经元细胞膜上电压门控性钠、钾、钙离子通道特性的影响，探讨听觉通路上神经元的电压门控性离子通道与耳鸣的关系。

The type, initial feeding time and concentration of precursor for HV unit formation had great effects on PHBV production in shake flasks. Propionate was selected as the better precursor for HV fraction than valerate after comprehensive survey of these two precursors. The suitable PHBV shaking-flask culture conditions were obtained through the analysis of PHBV fermentation process, the investigation of several factors by using RSA method and the fed-batch experiments. Cell dry weight, PHBV content, PHBV concentration, HV fraction and yield coefficient of HV unit to propionate were 27. 7g/L, 80.7%, 22. 4g/L, 16. 1mol% and 0.50g/g, respectively.

在共聚物PHBV的摇瓶发酵过程中，PHBV中HV组分形成的前体物质的种类、初始加入时间以及加入量是影响PHBV发酵过程的重要因素；在综合比较丙酸和戊酸这两种前体的基础上，选择丙酸作为HV组分合成前体较适宜；通过对PHBV发酵过程曲线分析、影响PHBV发酵指标的各因素的响应面分析以及摇瓶发酵补料试验，得到了较适宜的PHBV摇瓶发酵补料条件，在此条件下，细胞干重、PHBV含量、PHBV浓度、HV组分含量和HV组分对丙酸的产率系数可分别达到27.7g/L、80.7％、22.4g/L、16.1mo1％和

As mentioned earlier, elastinolysiss thought to be an essential component of emphysema.3 This is supported by both pathophysiologic and biochemical investigations, which show reduced lung elastic recoil, disrupted elastic tissue structure, higher circulating levels of elastin peptide breakdown products, and higher levels of desmosine in urine and lung secretions.3 Of the macrophage-derived MMPs identified in emphysematous and COPD tissue, the gelatinases MMP2 and MMP9 are elastinolytic.

正如早些时候提及的，elastinolysiss被认为是肺气肿的主要成份之一。这可以由病理生理研究与生化研究所证实，这两项研究同时提示肺弹性活力的降低，弹性组织结构的断裂，弹力蛋白肽分解产物循环水平的增高，以及尿与肺脏分泌物中锁链素水平的增高。在气肿型或COPD组织中，经确定的巨噬细胞源性的金属蛋白酶中，elastinoly的MMP2与 MMP9是elastinolytic。

The methods used for collecting samples and establishing specific HAB cultures are described in detail. Methods of SEM and ESEM (environmental scanning electron microscope) for Gymnodinium were developed an...

建立了赤潮监测中样品采集处理、分离和纯化培养的技术体系；建立和优化了裸甲藻扫描电镜观察技术和环境样品的扫描电镜观察方法；提出了库尔特计数是相对简便、快捷、准确、可行的细胞计数方法；应用基于反相高效液相色谱的光合色素分析技术及原位荧光激发光谱测定方法，得出不同类群浮游植物对不同的营养盐限制存在不同响应机制的结论；用荧光强度来监测浮游植物生理状态及营养状态变化具有一定的可行性，HPLC和原位荧光两种方法指示浮游植物的生理状态存在一定差异。2。

Electrophysciological indexes: in the experimental group ,the tardy rate of induced motorius action potential ,recovery rates of the amplitude of compound muscle action potential and twitch tension and tetanic tension of triceps surae muscle ,were significantly higher than in the control group.(4) Compared with the control group ,the triceps surae muscle wet weight was significantly greater in the experimental group.(5) histomorphological indexes: the count of myelinated nerve fibers ,diameter of regenerated axon,thickness of nerve myelin sheath and area of capillary were much more in the experimental group than in the control group.(6) observation of ultrastructure with the transmission election microscope: more abundant organella and maturer myelin sheath lay in the injured peripheral nerve of the experimental group, while not lay in the control group.

分别在术后第2、4、6周时进行各项指标的检测，结果发现：（1）实验组SD大鼠在整个实验过程中，其精神、毛发、体重，运动灵活性等一般情况优于对照组；（2）实验组的坐骨神经功能指数恢复率显著优于对照组，P＜0.01；（3）神经电生理指标：实验组运动神经诱发电位潜伏期的延迟率显著优于对照组，P＜0.05；复合肌肉动作电位振幅的恢复率显著高于对照组，两组比较第2周时P＜0.01，第4、6周时，P＜0.05；小腿三头肌单收缩力和强直收缩力的恢复率实验组显著高于对照组，P＜0.01；（4）小腿三头肌湿重的恢复率，第2、4周时实验组高于对照组，差异非常显著，P＜0.01；第6周时，差异仍显著，P＜0.05；（5）组织形态学指标：有髓神经轴突计数、再生轴突直径的恢复率，毛细血管面积，实验组均显著大于对照组，P＜0.01、P＜0.05、P＜0.001；实验组髓鞘厚度的恢复率也显著高于对照组，第2周时P＜0.01，第4、6周时，P＜0.05；（6）透射电镜超微结构观察：实验组再生轴突中细胞器丰富，髓鞘结构成熟，神经再生情况优于对照组。

The team developed a siRNA product that specifically blocks the transcription of the gene that codes for IL-8, thereby blocking production of this protein, and encases it in a liposome delivery system. In mouse studies, this product showed activity against 2 ovarian cancer cell lines, both when used alone and when used in combination with docetaxel. In addition, the mouse studies revealed that the product was decreasing tumor growth through anti-angiogenic mechanisms — the treated tumors showed a lower microvessel density, the researchers report. This product was "highly effective in our preclinical studies and may merit clinical development," they write. However, they also caution that the cell lines used to create the mouse models might not accurately reflect the heterogeneity seen in human tumors.

该团队发展一种siRNA产品，特别阻断IL-8的基因转译密码，因此阻断此一蛋白质之产生，且将它包入一个微脂粒传递系统；在老鼠研究中，不论单独使用或者和docetaxel并用，此产品显示对两种卵巢癌细胞线有活性；此外，老鼠研究发现此产品可透过抗血管新生机转减少肿瘤生长—报告指出，治疗的肿瘤显示较少的微血管密度，此产品在我们的临床前研究中相当有效，值得临床发展；不过，他们也表示，所用的老鼠研究中的细胞线可能无法精准反应人类肿瘤的异质性。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。