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Two different protocols for the isolation of nuclei and nuclear extracts from Arabidopsis plants and suspension cells are described.

描述了从拟南芥植物和悬浮细胞中提取细胞核和分离细胞核的两种不同的实验方案。

Now MSC become the chief seed-cell in cartilage tissue engineer, but the technology to stimulate MSC differentiate into chondroblast is not very successful,,the standard technology is culture the cell-ball in centrifuge tube,the reconstructured cartilage-like tissue is not achieve the mechanics of hyaline cartilage.

软骨组织工程的材料学研究主要体现在两类基质材料方面的进展,一类为天然基质材料,如胶原、壳聚糖、纤维素等;一类为人工合成可降解材料,如PGA、PLA等,但两者在来源、塑形能力及细胞识别信号等方面存在不足。

Tissue factor, a receptor on the cell surface of factor VII as well as a coactive factor to activate factor VII, plays an essential role in hemostasis through the activation of the intrinsic and extrinsic coagulation pathways simultaneously.

组织因子(Tissue factor,TF)既是Ⅶ因子在细胞表面的受体,又是激活Ⅶ因子的辅助因子,它可同时启动内外两条凝血途径,在生理性止血中起重要作用。

Dentata. The cytotaxonomical value of the features of karyotypic structure and the positinal differentiation of NORs between this two spepies is preliminarily discussed.

作者对这两种摄龟核型的结构特点和NOR位置差异的细胞分类学意义进行了讨论。G[2]G

Based on reviewing of functionalization of saturated carbon by catalytic oxidation with metalloporphyrins and metal phthalocyanines as a mimic of the enzyme cytochrome P-450, two reaction systems of which one was the preparation of 4, 4'-dinitrostilbene-2, 2'-disulfonic acid from 4-nitrotoluene2-sulfonic acid and the other was the preparation of 4, 4'-bis (dimethylamino -diphenyl) phenylmethanol from 4, 4'-bis (dimethylamino-diphenyl) phenyl methane , were specifically studied as carfunctionalization and oxyfunctionalization of saturated carbon.

本文在全面综述金属卟啉和金属酞菁类模拟细胞色素P-450酶催化剂催化氧化饱和碳原子功能化的基础上,选择由4-硝基甲苯-2-磺酸制取4,4'-二硝基二苯乙烯-2,2'-二磺酸和由4,4'-二苯基甲烷制取4,4'-二苯基甲醇两个反应体系进行饱和碳原子碳功能化和氧功能化的具体研究。

Next, seven of those trimmed molecules combine to form a ring-shaped structure, or heptamer, that captures the two factors and is transported to an internal membrane-bound compartment called an endosome.

之后,七个剪裁过的保护抗原分子会聚集起来,形成一个环状构造,称为「七聚体」,藉此抓住另外两种因子,再一起送入细胞内一个称为「内小体」的膜状构造。

In the growth curve experiment, it is found that TB has caused more significant growth inhibition in A431 human epidermoid carcinoma cells than in Hs695T and RPMI 7951 human melanoma cells.

在生长曲线的实验我发现,相较於Hs695T和RPMI 7951这两株人类黑色素瘤细胞,TB抑制人类表皮癌细胞A431的生长效果较为明显。

The divergence time between C.amherstiae and C.pictus was at least 1.7My based on the substitution rate of nucleotide acid of cytothrome-b gene in Galliformes.

根据鸡形目细胞色素b基因核苷酸替换的速率约为每百万年0.5%~ 0.7%,推断出两种锦鸡的分化时间至少为1.7百万年。

First passage of human amniotic epithelial cells were inoculated at a density of 1×108/L and cultured in medium containing fetal bovine serum of 0.1 volume fraction, 100 nmol/L dexamethasone, 50 mg/L ascorbic acid and 5 mmol/L β-glycerophosphate.

取第1代人羊膜上皮细胞,设立两组:诱导组以1×108 L-1密度接种于培养皿内,24 h后更换为含体积分数为0.1的胎牛血清、 100 nmol/L地塞米松、50 mg/L抗坏血酸、5 mmol/Lβ-甘油磷酸的诱导培养液。

Both vectors were stable in host cells tested and could be generally useful for expressing heterologous genes.

两种载体在宿主细胞中是稳定的,应可用于异源基因的表达。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。