两细胞的

Following the puncture of zona pellucida with two holes by injection pipette that contained donor nuclei or cells, the injection pipette was pulled back to the perivitelline space while the negative pressure was increased in the holding pipette until the polar body and karyoplasm were wiped off completely. Then a reconstructed embryo was completed by the direct injection of the donor nucleus or cell without pulling out the injection pipette.

首先以预先吸有细胞核或细胞的注射针在固定于持卵针上的卵母细胞透明带上穿刺两个孔，然后一边缓慢地将注射针回拔至卵周隙中，一边逐渐增加持卵针中的负压，直至极体与目标核质被完整吸入持卵针中而完成去核，最后在不拔出注射针的情况下直接注射细胞核或完整细胞进而完成重构胚的构建。

Results ①Arsenicals caused inhibition obviously on HL-60 cells growth at the test concentrations,particularly in the groups of 2μmol/L,10μmol/L sodium arsenite,and the groups of 100 μmol/L,300 μmol/L sodium arsenate.

结果①两种无机砷化物对HL-60细胞生长具有抑制作用，特别是高浓度NaAsO2（2、10 μmol/L）和Na3AsO4（100、300 μmol/L）可明显地抑制HL-60细胞的生长

12H treatment group compared with 12h model group: the level of MCP-1 of the Gardenoside group decreased and MIP-1 is no different with the model group; the level of the MCP-1, MIP-1 of the Baicalin group are no different with the model group; the level of MCP-1, MIP-1 of the Concha Margatitifera Usta decreased than the model; the level of MCP-1 of the Cholic Acid group is no different and the level of MIP-1 decreased than the model; the level of MCP-1 of the Hefang Group decreased and the level of MIP-1 of it decreased than the model group.

合方治疗脑缺血24小时组缺血脑组织nNos光密度值较缺血24小时模型组明显降低（p＜0.01）。细胞的死亡分为坏死和凋亡两种方式，凋亡是细胞为避免对机体造成更大损伤而采取的一种程序性的死亡方式。珍珠母、胆酸、栀子苷、黄芩苷、合方治疗组较脑缺血24小时模型组可明显降低P53阳性表达，考虑药物抑制细胞凋亡可以起到对缺血脑组织的保护作用。

The responses of osteoblasts to both agents can be as the characteristics of primary osteoblast derived from rat calvaria.

这些成骨细胞对上述两种药约之反应也可作为由大鼠头颅骨所取出之成骨细胞的特性。

The endocellular Fura-2 increased to 2.39,3.14,and 3.24 fold respectively by addition of AA,bullatacin and atemoyacin-A and the accumulation of Dox in MCF-7/ADR cells was similar to that of Fura-2,increased to 2.98,4.10 and 4.24 fold respectively by AA,bullatacin,and atemoyacin-A while no in MCF-7 cells.

细胞内阿霉素的积累与Fura-2 相似，加入AA，bullatacin和atemoyacin-A均分别增加 MCF-7/ADR 细胞内 ADR 的积累至2.98，4.10和4.24倍，而未见显著增加敏感株内 ADR 的积累。bullatacin和atemoyacin-A均能诱导MCF-7与MCF-7/ADR细胞的凋亡，且相同浓度下，两细胞株的凋亡百分数无显著差异。

The shortage of this way was that feeder cells could be passaged together with hESCConclusion HAFi could replace MEF as feeder layer to support the long-term culture oftwo Chinese human embryonic stem cell lines. This could remove the cells of animal originand heterogenetic pollution in the culture system. It also reduced the culture of primarycells and solved the instability caused by different batches of primary cells.

结论HAFi细胞可以用做两株中国人胚胎干细胞培养的饲养层，取代MEF后解决了动物源性细胞混杂和异源污染问题，省却了饲养层原代细胞的培养步骤同时解决了由不同批次饲养层差异带来的培养体系的不稳定。

When the cytotoxic activity of these two types of TNF was compared, the synthesized 26kD TNF was found to exert its effects only in the presence of microsomal membranes.

比较这两种体外表达产物对靶细胞的细胞毒效应，结果表明，只有在微粒体存在时所合成的26kD TNF具有细胞毒活性；而无微粒体存在所合成的裸26kD TNF不显示杀瘤效应。

With the help of dual culture technology, we found that the auxiliary cells with or without spinules presented two different development stages, but not different morphology as had been suggested previously.

在原位观察的过程中，纠正了一些形态上的模糊认识，如表面有突起的与表面光滑的辅助细胞是形成过程中的两个阶段，而不是辅助细胞的两种形态。

It is possible to preparing neutralizing MAb which target to active region of Stx2 by synthetical B cell epitope peptides. At present, the crystal structure of the compound of Stx2 and its ligand adenines has been resolved, and the active site of Stx2 is definite, which is the seventy-seventh amino acids, Ganimalon, of Stx2 A1 fragment.

Stx2通过B亚基与受体Gb3结合后，进入细胞，在弗林蛋白酶(一种钙敏感的丝氨酸蛋白酶)作用下A亚基被裂解为A1和A2两个片段，A1片断经内质网进入细胞质暴露毒性活性位点―A1片段发挥N-糖苷酶活性，导致真核细胞蛋白质合成的终止，最终导致细胞的死亡。

In the third part, cultured H9C2 myocytes were randomly divided into two groups, the control group and 200mol/L DZ preconditioning group.

第三部分，培养的H9C2心肌细胞随机分成2组，即对照组和200mol/L DZ预处理组，分别富集两组细胞的磷酸化蛋白质，采用双向电泳和质谱技术，分离鉴定DZ预处理后心肌细胞内磷酸化蛋白的差异表达。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。