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In this study the maize transposable element Activator was introduced into Nicotiana tabacum L cultivar Nc89 and its haploid.

本研究把玉米转座因子Ac分别转化烟草二倍体和单倍体,研究Ac在这两种细胞中的转座行为。

Methods: Two recombinant palter plasmid containing whole CDNA sequences of HMCD59 transfected CHO cells by lipofectamine with PCDNA respectively. Then G418 was used to select positive clone.

分别构建两种含有HMCD59全长CDNA序列的重组pALTER质粒,并与pCDNA3质粒,按3:1比例混合后,运用脂质体介导法共转染CHO细胞(编号为HM5-CHO及HM6-CHO),用新霉素类似物G418初步筛选出阳性克隆。

The results showed:among the 7 isolates, five isolates of HY3、GY1-3、ZJ1-1、HP1、FC3 had same colony shape, irregular shape, liquidlike, slimy, opacity with smooth surface;the other two isolates had same shape, irregular shape, dry, opacity with coarse surface. By inoculating eucalyptus with the 7 isolates, the plants were infected apparently, and the young plants of eucalyptus in control experiment with tap water were not infected. By cultivating eucalyptus cuttings with the bacterial suspensions without EPS, the incidence of disease was very distinct,but compared with the former bacteria suspension,the incidence of disease has decreased at different degrees. By screening out two isolates of strong pathogenicity and two isolates of weak pathogenicity from the 7 isolates,making the bacterial suspensions with them to inoculate the young plants of eucalyptus, two treatments of cutlings and ramets with rats were set with 5 repetitions in every treatment, the results of data analysis showed: for the cutlings, the bacterial contents in upper and middle parts、upper and lower had significant difference;for ramets with roots, the bacterial contents in upper, middle parts, lower had significant difference between each other; For both the cutlings and ramets with roof, the bacterial contents in xylem and phloem had significant difference. The interaction between vertical and horizontal parts for the bacterial content had significant difference. For the two isolates of HY3 and 93B which were screened out at last,their activities of the cellulase were: 1.955ug/ and 1.288ug/ respectively, and had significant difference; the activities of pectase were: 1.325 ug/and 1.24ug/ respectively, and had no significant difference. The content of EPS extracted from the two isolates of HY3 and 93B was very different: 7.08x10-8ug/cell and 5.17x10-8ug/cell.

结果显示:7个菌株中,其中5个菌株HY3、GY1-3、ZJ1-1、HP1、FC3的菌落形态相同:不规则形状、流体、粘性、不透明、表面光滑;另外2个菌株93B、GN1菌落形态相同:不规则形状、干燥、不透明、表面粗糙;用7个菌株接种剪根桉树苗,发病情况非常明显,而自来水对照实验中桉树苗却不发病;无EPS菌悬液培养桉树剪根苗,发病率也很明显,但是相比原菌液,则发病率有不同程度的下降;从7个菌株中间筛选出来2个强致病性菌株和2个弱致病性菌株,用它们配制菌悬液培养桉树苗,设置剪根和不剪根两个处理,每个处理设置五个重复,数据分析结果显示:对于剪根苗,上部和中部、上部和下部的含菌量有显著的差异,中部和下部含菌量差异不显著;带根苗,上部、中部、下部含菌量彼此之间差异显著;不管是剪根苗还是带根苗,木质部和韧皮部含菌量之间的差异都非常显著;上中下与木韧交互作用中,含菌量差异非常显著;最后筛选出来的强弱2个菌株HY3和93B,它们的纤维素酶活性分别为:1.955ug/和1.288ug/,具有显著的差别;果胶酶的活性分别为:1.325 ug/和1.24ug/,没有显著的差别,而且HY3和93B两个菌株细胞分泌的胞外多糖的含量差异很显著,分别为:7.08×10-8ug/cell和5.17×10-8ug/cell。

Results: The permillage of apoptotic myocytes in infarct area increased at 1 h and achieved the peak at 4 h, and then decreased to ...

两种基因mRNA表达量的对数值之间呈负相关,心肌细胞凋亡数与Bac/Bcl 2基因mRNA比值呈正相关。

The mechanisms and applications of cell electrofusion were studied systematically in this PH. D dissertation in following four parts

本文从理论和应用两方面对细胞电融合技术进行了比较广泛和系统的研究,主要包括

However,the individual role and relationship between these two signals on the clearane of apoptotic cells by phagocyte remain unclear.

但两种信号在凋亡细胞被吞噬细胞识别和清除中的作用和相互关系目前还不清除。

The identified proteins were divided into two following categories: one is regulatory proteins, for example, caffeic acid 3-O-methyltransferas, HSP70, glyoxalase I , actin, and so on. They are involved in protein biosynthesis and processing, cellular structural organization and stress responses. The other is function proteins, for instance, ascorbate peroxidase, cytoplasmic malate dehydrogenase, cytosolic phosphoglycerate kinase, S-adenosyl methionine synthetase, ATP synthase, and so on. They are involved in photosynthesis, photorespiration, redox homeostasis, and metabolisms.

通过分析可以将这些差异蛋白分为两大类:第一类是调节性蛋白,如咖啡酸三氧转甲基酶,热激蛋白70,醛酮变位酶I,肌动蛋白等,它们主要参与蛋白质的合成和加工、胁迫响应、细胞骨架稳定等过程;第二类是功能性蛋白,如抗坏血酸过氧化物酶,细胞质苹果酸脱氢酶,细胞质磷酸甘油酸激酶,S-腺苷蛋氨酸合成酶,ATP合酶等,它们则参与氧化还原平衡、光合作用、光呼吸和各种代谢等过程。

MAIN OUTCOME MEASURES: Phosphorylation of neurofilaments in both groups.

主要观察指标:两组细胞内神经细丝磷酸化的程度。

Experiment on Visual cell by nature and men:in search of treatment for photoreceptor degeneration.

两种低强度微波辐射致兔晶状体上皮细胞改变的定量研究。

MMP25 may play an important role in embryo implantation and placentation.

蜕膜组织中MMP25主要在腺上皮细胞表达,自然流产组的表达微弱,两组平均光密度分别为0.25±0.13和0.21±0.12,差异有统计学意义(P<0.05)。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。