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Methods: Eleven B-CLL patients were studied. Leukemic lymphocytes with (n=8) or without (n=3) P2z receptors were exposed in vitro to ATP, benzoylbenzoic-ATP, 2-methylthio-ATP(2MeSATP), adenosine-5′-[γ-thio] triphosphate, and other nucleosides for 8h. Apoptosis was detected by electron microscopy, agarose gel electrophoresis, and quantitative TdT assay. Results:Apoptosis was detected only in leukemic lymphocytes with P2z receptors. By using the quantitative assay, ATP-inducing DNA strand breaks were found to occur specifically for BzATP, ATP and 2MeSATP, but not for ATP-γS and other nucleosides.

将表达P2z受体[P2z]与不含P2z受体[P2z]的两组CLL细胞分别同1.0mmol/L三磷酸腺苷体外培养8小时,以电镜、DNA凝胶电泳和定量DNA 3′端TdT法检测细胞凋亡;并对ATP、苯甲酰苯甲酸ATP、2-甲基硫ATP(2MeSATP)、γ-硫代ATP及其它核苷的诱导效应和氧化型ATP、1-[N,O-二(5-异喹啉碘酰基)N-甲基-L-酪氨酰]-4-苯哌嗪KN-62)的抑制效应做定量研究。

The two major events occurred in the beginning stage of reprogramming are: the Zygotic Gene Activation in 1-cell embryos and the formation of a Transcriptionally Repressive State during 2-cell stage.

其中最明显的两个变化是出现于一细胞胚胎中的合子基因表达激活与出现于二细胞胚胎中的转录抑制。

Two key advantages of functionalized carbon nanotubes are their strong ability to translocate through plasma membranes and low toxicity, which make their applications in drug and gene transfer systems being possible.

功能化CNTs的两个关键优势在于它具有很强的细胞穿透能力和较低的细胞毒性,使其在药物和基因转运领域中的应用成为可能。

The results showed that some holes on the nuclear membrane of the two kinds of the cells besides normal cell structures and there are some secretive pellets of protein with more high density of electronics around the nuclear of the cells, these pellets maybe is the regulatory matter of living things growth and around o'clock section rule, It is important to sturdy the ultrastructure of brain tissues and the physiological function of Tupaia belangeri chinensis .

利用电镜观察了树鼠句Tupaia belangeri chinensis的脑垂体和松果体细胞的超微结构,结果显示这两类细胞除了有正常细胞结构外,核膜孔较大且在核周围还有一些电子密度不同,大小不一的蛋白质分泌颗粒,这些颗粒可能是生物个体调节各种生理生化功能、生长发育和昼夜节律的信使物质,这对进一步研究树鼠句脑组织结构及其生理功能有一定意义

We find that there are two types of CRF immunoreactive neurons in the cerebral cortex. Type I cells were mainly distributed in layers II -III. Morphologically this type of cells is interneuron and is the true CRF neuron in general meaning.

我们的研究显示,皮层中有两种类型的CRF阳性神经元,其中CRF阳性Ⅰ型细胞主要分布在皮层的Ⅱ-Ⅲ层,形态学特征显示这些细胞为抑制性中间神经元,且为真正的CRF神经元。

Na-alginate was selected as an entrapping agent to immobilize ammonia-oxidizing bacteria in this experiment, designed to explore optimal entrapping condition and to compare immobilized bacteria with non-immobilized bacteria in ammonium removal efficiency.

以海藻酸钠作为氨氧化细菌的包埋固定载体,以固定化氨氧化细菌对氨氮的去除率为主要指标,研究氨氧化细菌的最佳包埋条件,并比较了未固定细胞与固定化细胞两种处理对水中氨氮去除效果的影响。

Objective To study the effects of berbamine and batiolun on LDH metabolizability in the process of increasing leukocyte.Methods Enzyme biochemical techniques is used to study the effect of berbamine and batiolˉun in the process of inceasing leukocyte and also to study the effect of LDH metabolizability.

目的 研究小檗胺和鲨肝醇升高白细胞作用及对细胞内LDH的代谢的影响方法采用酶细胞化学方法,对两药物在治疗由免疫抑制剂环磷酰胺所致的白细胞减少症时,对乳酸脱氢酶活性的影响进行了比较观察。

The array chip takes a flexible transparent polyimide thin membrane as the base, the lower surface can form a cross comb-shaped array multimicroelectrode, the electrode group is composed of two comb-shaped microelectrode array electrodes which are mutually crossed but not contacted with the electrical structures not being connected, and the microchannel between the both internal electrodes of the electrode group serves as the service passage; the array chip is inversely buckled on the fusion pool, and the cross comb-shaped array multimicroelectrode on the array chip correspondes to the cell electric fusion pool and falls in the cell electric fusion pool.

阵列芯片以柔性透明聚酰亚胺薄膜为基底,下表面通过蚀刻形成交叉梳状阵列化微电极组,电极组由两个相互交叉、互不接触、电气结构上互不连接的梳状微电极阵列电极构成,电极组内部微电极之间的微通道为工作通道;阵列芯片倒扣于融合池上,其上的交叉梳状阵列化微电极组与细胞电融合池相对应,落于细胞电融合池中。

Two phylogenetic footprints, 35 bp and 17 bp in length respectively, were found in 5' intron sequence.

在内含子的5'端发现了两个高度保守的序列——进化印记,长度分别为35 bp和17 bp,35 bp的核苷酸片段能与HeLa细胞和鸡胚细胞抽提物中的蛋白结合,可能是一种调节元件。

PartⅡExperimental Study on Acute Hypatocyte Necrosis and Apoptosis with MRI, DWI and MRS Objective: To demonstrate the ability and value of MR imaging, diffusion-weighted imaging, and MR spectroscopy in detecting and characterizing necrosis and apoptosis in vivo with a comparison to the pathology after establishment of hepatic necrosis and apoptosis animal model by different surgical ligations of hepatic blood supply vessels.

目的:通过手术结扎的方法建立大鼠肝细胞坏死和凋亡的动物模型,将不同时间点的MRI、DWI和MRS检测结果与病理学所见进行对照研究,以评价MRI、DWI及MRS在检测及鉴别肝细胞凋亡和坏死两种病理改变的能力与价值。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。