两细胞的

After cultured with tumor cytolysis antigen for 2 days, the cells were incubated with auto T cell separated by E-rosette formation. Methyl thiazolyl tetrazolium assay was employed to test inhibition rate of tumor specific cytotoxic T lymphocyte on HO9810 tumor cell line. Results Our results indicated that DC precursors in cord blood can be induced to differentiate and mature in the medium containing IL-4, GM-CSF, and TNF-α.

将获得的DC与用 E-花环分离脐血单个核细胞得到的T淋巴细胞共同培养3 d，然后分为两组，实验组2继续与不同比例的HO9810细胞共同培养3 d后获得细胞毒T淋巴细胞，对照组2除加入HO9810外，还加入了无关细胞，用四甲基偶氮唑蓝法检测其对HO9810细胞的抑制作用。

Therefore, in order to find out the most important bioactivity of Hoshouwu, the present research chooses cell regeneration and immunomodulation as platforms for validation. The bone marrow cavity consists of two rapidly regenerating systems: hematopoiesis and bone remodeling process.

骨髓是人体持续进行细胞再生作用最活跃的区域之一，本论文分别以体外细胞培养及动物实验方式测试何首乌成分对骨髓中具有细胞再生功能的两大族群：造血干细胞和基质细胞的相关影响。

The results showed that cMSCs and EA.Hy926 endothelial cells could attach and proliferate on scaffold, and the cell did not appear obvious abnormity in morphology. So it was indicated that the scaffold could not disturb the biological behavior of cMSCs and EA.Hy926 endothelial cells. It also showed that cells in dynamic culture were growing more bloomingly and metabolizing more rapidly than those in static culture.

两组研究结果发现细胞能在支架材料上粘附、增殖，并且细胞形貌正常，说明材料的加入不影响细胞的生物学行为，同时发现，动态培养的细胞比静态培养的细胞生长旺盛，代谢快。

Conclusions1、E_2 may induce the apoptosis and Fas,FasL expressions of Jurkat cell,its effect positively related to the dose and treating time of E_2.2、There is intrinsic co-expression of ERαand ERβon Jurkat cell,which can be upregulated by E_2,positively related to E_2 doses.3、E_2 may act as an apoptosis inducer of Jurkat cell via ERβ.4、E_2 can promote the transcriptive activity of NF-κB in Jurkat cell.The increased NF-κB activity may lead to increased apoptosis of Jurkat cell.

结论1、E_2可增加Jurkat细胞的凋亡，并增加Fas、FasL的蛋白表达，且其作用与E_2的剂量和处理时间呈正相关。2、Jurkat细胞上存在ERα、ERβ的表达，且E_2可上调这两种受体的表达，E_2剂量越大，ER表达量越高。3、E_2可能通过ERβ发挥其促进Jurkat细胞凋亡的作用。4、E_2能增加Jurkat细胞中NF-κB的转录活性，Jurkat细胞中NF-κB活性增加有促进凋亡的作用。

This suggest that high-oncogenic-risk HPV and HSV-2 can be cofactors during the development of cervical cancer.

观察比较H16，HN和HB三种细胞生长特征，结果发现HN和HB细胞的生长饱和密度皆约两倍于H16细胞。

Part III: Peripheral blood dendritic cells in coronary atheroscleroticdiseaseObjectives: Dendritic cells subsets, Myeloid DC and plasmacytoid DC, regulate immune reactions by polarising naive T-helper cells into a Th1 or Th2 response, respectively.

第三部分：冠心病患者外周血树突状细胞亚型的变化的研究目的：树突状细胞的两个亚型，髓样DC和浆细胞样DC分别是促使T辅助细胞向Th1和Th2免疫应答转化的抗原呈递细胞。

The total proteins from TGIF transfectant and vector control cells were separated by 2D electrophorosis. 760±41 and 680±38 spots were detected in TGIF transfectant and vector control cells respectively. Locus repeat analysis of protein spots showed that the mean deviation in IEF direction in TGIF transfectant and vector control cells were 0.864±0.123 mm and 0.843±0.115 mm respectively whereas the mean deviation in SDS-PAGE direction in these two cells were 0.812±0.109 mm and 1. 125±0.123 mm respectively. So the well-resolved and reproducible 2DE patterns from TGIF transfectant and vector control cells were established.

通过双向凝胶电泳分别分离TGIF转染细胞和PcDNA3.1空白质粒转染细胞的总蛋白质，测得两组细胞的蛋白质斑点数分别为760±41和680±38个；位置重复性分析表明TGIF转染细胞在等电聚焦方向上的平均偏差为0.864±0.123mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为0.812±0.109mm；PcDNA3.1空白质粒转染细胞在IEF方向上的平均偏差为0.843±0.115mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为1.125±0.123mm。

In control group,Ⅰ tail current density of Epi myocytes was 49.1% bigger than that of Mid myocytes, in operation group, which is 77.6%.Ⅰ tail current density had no significant difference among three layers in two groups.

对照组外膜细胞的Ⅰ(下标 Ks尾电流密度较中层细胞大49.1%，而手术组为77.6%；两组三层细胞之间Ⅰ(下标 Kr尾电流密度均无差别。

However, when two maturing oocytes fused and two spindle will form in the big cell, the chromosomes will not intermingle. In the present study, we removed GV from one oocyte and transfer to the perivetelline space of another GV oocyte. After fusion the resulting oocytes which contained two GVs were cultured further in MEM.

在本实验中，我们利用小鼠GV期卵母细胞将一枚卵母细胞的生发泡取出后移至另一未经去核处理的GV期卵母细胞透明带下，经三次电脉冲作用后将融合的含有两个GV的卵母细胞放入MEM成熟培养液中培养，在培养成熟的不同阶段分别收集卵母细胞进行免疫荧光染色观察微管及核的变化。

The results showed that after 2 h of culture in MEM the chromosomes of oocyte were seperated to form telophase I while a small spindle was observed around chromosomes of primary spermatocyte. However, two clear spindles were observed in the oocytes cultured in CB containing MEM. After further culture, the chromosomes of both primary spermatocyte and oocyte intermingled and formed one large spindle.

在无CB的培养液中培养的卵母细胞培养2小时后，卵母细胞已经进入第一次减数分裂的后期，染色体开始被拉向两极，而精母细胞的MI纺锤体才刚刚形成，虽然继续培养两者染色体可以合二为一并形成一个纺锤体，但是有些染色体发生滞后；当卵母细胞在含有CB的培养液中培养2小时后，在卵母细胞内形成两个相似大小的MI纺锤体，进一步培养形成一个大的纺锤体，染色体正常。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。