两细胞的

In contrast, nasal administration of a mixture of MBP6886 and 8799 completely blocked gpMBPinduced EAE even at lower dosage than being used alone. Rats tolerized with MBP6886+8799 nasally showed decreased T cell responses to MBP, reflected by lymphocyte proliferation and IFNγ ELISPOT assays.

淋巴细胞增殖实验和IFNγ ELISPOT检测显示，与鼻黏膜给予大鼠MBP110128组相比，鼻黏膜给予大鼠MBP6886+8799可降低T细胞对于MBP的反应性，淋巴结单核细胞中表达IFNγ和TNFα mRNA的细胞数减少，而两组表达TGFβ及IL4 mRNA的淋巴细胞数都低。

The sensitization system of an ommatidium included 11 retinular cells, of which four located at the distal part of the ommatidium; seven formed the proximal main part of the retina. The two parts are some one on top of another.

透射电镜观察显示红螯螯虾小眼的感光系统由11个小网膜细胞组成，其中4个小网膜细胞位于晶锥之下，构成了感光部分的远端，7个小网膜细胞构成感光系统的近端主体，上下两部分有重叠。

Cytoembryological studies were employed to reveal theorigin of haploid and to determine the cytological factersrelating to the production of diploid polyembryony withinone embryo sac occurring in natural population of apolyembryonic line SB-1 of O.sativa.

SB-1自然群体中的单倍体来源于助细胞无配子生殖。细胞胚胎学研究表明，卵器中的一个或两个助细胞能在受精之前发育成胚，其发生率分别为观察子房数的2.24%和1.75%。

RESULTS: The AF cells cultured in different substrates were morphologically undistinguishable. Toluidine blue staining showed that there was also no difference between AF cells cultured on flexible silicone membranes and in plastic plates. The cells growing in different substrates expressed the same levels of collagen typeⅠ,Ⅱ and aggrecan mRNA and integrin β1. AF cells grew well on silicone membranes.

结果：种植于不同基质的纤维环细胞形态学观察无明显差别；在两种基质上的纤维环细胞甲苯胺蓝染色无差异；Ⅰ，Ⅱ型胶原及聚集蛋白聚糖mRNA及细胞膜整合素β1的表达亦无明显差异；纤维环细胞可以黏附于硅橡胶膜上生长。

So we selected the colchicine optimumparameters were 0.2μg/ml and 6 hour cultured duration.The third, there were significantly differences percent between 10 hourgroups and other three groups (4 hour groups, 6 hour groups and 8 hour groups) in0.1μg/ml groups; and there were significantly differences between 8 hour groupsand 10 hour groups, but they had no significantly differences among other twogroups in 0.3μg/ml groups; and there were significantly differences between 10hour groups and 6 hour groups, but 10 hour groups had no significantlydifferences among other two groups in 0.5μg/ml groups; and there weresignificantly differences between 8 hour groups and other three groups in0.7μg/ml groups on the metaphases (P＜0.05), in the experiment of the effect onthe mouse 4- cell embryo stage single blastomere of vinblastine with differentconcentrations and duration, by x~2 statistical analysis.

根据实验的实际情况，适宜制备小鼠8-细胞期胚胎单卵裂球染色体标本的秋水仙素的处理浓度和时间是0.2μg／ml和6小时。3、在确定长春花碱不同处理浓度和时间对小鼠4-细胞期胚胎单卵裂球中期分裂相数影响的实验中，经统计分析，阻断培养0.1μg／ml浓度组中10小时组与其他三组差异显著(4小时组、6小时组和8小时组)；阻断培养0.3μg／ml浓度组中8小时组和10小时组间差异不显著，但与其他两组差异显著；阻断培养0.5μg／ml浓度组中10小时组与6小时组差异不显著，而与其他两组差异显著；阻断培养0.7μg／ml浓度组中8小时组与其他三组差异显著(P＜0.05)。

Heart malformation, such as PTA, DORV, VSD and aortic arch malformation, were detected when neural crest were taken out before they migrated into 3, 4, 6 pharyngeal gland. Interestingly, the types of malformation were related with the length of neural crest destroyed; PTA was detected if the length exceeds two somites, while DORV would be detected if the length less than two somites. These results indicate that CNCCs are very important to the conotruncal development.

研究发现，如果在迁移之前切除散发到第3、4、6咽弓的神经嵴细胞，就会出现永存动脉干、主动脉骑跨、右室双出口、主动脉弓畸型和室间隔缺损等心血管畸形，且切除的长度与畸形的种类相关，如切除长度大于两个体节可产生永存动脉干，切除长度小于两个体节则产生右室双流出道，由此可见，心脏神经嵴细胞对于心脏圆锥部的发育意义重大。

Oocyte samples from one group were collected to detect the presence and integration of HBV DNA within cells and chromosomes using PCR, Southern blot, dot hybridization and fluorescence in situ hybridization. The female animals from another group were mated with their normal males, respectively. Their zygotes, 2-cell embryos were collected to detect the integration of HBV DNA in the female pronuclei of zygotes and the replication and expression of HBV genes in the 2-cell embryos using FISH, RT-PCR and immunofluoresence assay.(1) PCR detected positive bands in the tested oocyte samples fromgoldon hamster and mice. Southern blot revealed clear hybridization signals in PCR products.

研究用金黄地鼠和小鼠建立实验动物模型：将卵巢内注射HBV DNA的实验动物分成两组，一组注射后进行超排卵，收集卵巢和输卵管的卵母细胞，用PCR、Southern杂交，斑点杂交和荧光原位杂交(fluorescence in situ hybridization、FISH)检测HBV在卵母细胞内的存在和染色体上的整合；另一组超排雌鼠与正常雄鼠合笼，收集受精卵和2-细胞胚，用FISH、RT-PCR和免疫荧光检测技术分别研究HBV基因在受精卵雌原核上的整合以及在2-细胞胚中的复制与表达。

The results of transient transfection showed that the recombinant plasmid could be lowly expressed in the murine macrophage cell line RAW264.7 and murine melanoma cell line B16, both of which can endogenously express Tim-3, but not in the African green monkey kidney cell line COS-7 in which no endogenous Tim-3 could be detected.

结果 pGL3-Tim-3P2经PCR、双酶切及DNA测序分析鉴定正确无误；pGL3-Tim-3P2转染RAW264.7和B16细胞（两种细胞均可表达内源性Tim-3）24 h和48h，双荧光素酶活性检测显示其启动子相对活性约为pGL3-Basic空载体的2倍；而pGL3-Tim-3P2转染不表达内源性Tim-3的COS-7细胞后检测不到荧光素酶活性。

Methods: Oocytes in the GV stage were separated from ovary by squeezing method. In mouse germinal vesicle GV stage, the expression of ATP8 gene in the mitochondria in the single oocyte was detected by RT-PCR, in which, cDNA was synthesized with two methods: one was the single GV-stage oocyte directly to be placed RT, the other was to perform RT after eliminating mtDNA and nucleus DNA with the EeoR Ⅰ enzyme and Dnase. And the product of RT-PCR was cloned and sequenced.

应用挤压法从卵巢中分离获得生发泡期(germinal vesicle， GV)卵母细胞；用RT-PCR检测GV期单个卵母细胞中ATP8基因的表达：其中cDNA的合成分两种方法进行：一是将GV期单个卵母细胞直接进行RT合成cDNA，二是先用DNA酶加EcoR Ⅰ酶祛除mtDNA和核DNA后再进行RT；回收产物构建克隆质粒并测序。

Fission A type of asexual reproduction in which a parent cell divides into two daughter cells genetically identical to the parent. Binary fission occurs in many unicellular organisms, such as protoctists, bacteria, and fission yeasts.

裂殖：无性生殖的一种类型，指一个母细胞分裂成两个与母细胞相同的子细胞，二分裂发生在许多单细胞有机体中，如原生生物、细菌和裂殖酵母。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。