两细胞的

The results showed that 1 there were no significant differences in the rates of cytoplast protrusion and enucleation between oocytes that were incubated in colchicine (0.4 μg/mL) for 0.5 h and oocytes that were incubated in colchicine (0.4 μg/mL) for 1 h, and the rate of cytoplast protusion can be 85.4% while the rate of cytoplast enucleation is 100%. 2 There was no significant difference in oocyte enucleation between oocytes treated with medium containing 0.2 μg/mL colchicine for 0.5 h and oocytes treated with medium containing 0.4 μg/mL colchicine for 0.5 h. 3 A maturation time of 18–23 h did not affect the rates of cytoplast protusion and enucleation by chemically assisted enucleation, whereas the rate of enucleation of oocytes by blind enucleation was found to decrease with a prolonged incubation time. 4 The development rates of reconstructed embryos could not be influenced by these two enucleation methods, increased from oocytes matured for 21–23 h.

结果表明： 1 卵母细胞在0.4 mg/mL的秋水仙素溶液中分别孵育0.5 h和1 h，胞质突起率和去核率没有显著的差异，突起率可高达85.4%，去核率达到100%； 2 0.2 mg/mL或0.4 mg/mL秋水仙素溶液将卵母细胞处理0.5 h，对去核效果没有显著影响； 3 对于体外成熟18~23 h的卵母细胞，随着成熟时间的延长，盲吸法的去核率降低，但没有影响秋水仙素诱导胞质突起的比率和去核率； 4 两种去核方法对重构胚的发育没有产生显著影响，但成熟21~23 h卵母细胞重构胚囊胚的发育率显著高于成熟18~20 h卵母细胞重构胚囊胚的发育率。

The differentiation and fusion of cells may be therefore the two essential ways for the BMDSCs to change their phenotypes from mesoderm to ectoderm.

细胞转分化和细胞融合有可能是中胚层来源的BMDSCs向神经外胚层细胞变化的两种必要机制。

Other reported cases in the prostate include a clear cell carcinoma of the prostatic utricle in a 16 year old boy reported from Uruguay and a renal cell type of prostatic carcinoma which lacked hobnail cells and had staining patterns similar to renal cell carcinoma.

其他有关前列腺透明细胞癌的报道有两例，一例发生在乌拉圭的一个16岁男孩的前列腺囊，另一例报道为前列腺癌的肾细胞型，此类型没有靴钉样细胞，而是与肾细胞癌相似的染色形式。

Our aim was to investigate the effect of malate and GABA on telomerase activity of Hela cells and ex-plore the relationship of telomerase activity change with growth, metabolization and deterioration of cancer cells to provide new tactics for treatment of malignant tumor.

Hela细胞是研究端粒酶的常用细胞，本研究旨在观察苹果酸和GABA这两种作用因素对Hela细胞端粒酶活性的影响，探讨端粒酶活性的变化与二者在肿瘤细胞生长、代谢和恶化方面作用关系，为恶性肿瘤的治疗提供新的策略。

The quantities of starch grains in the two"cells"in micropyle part of embryosac gradually decrease and become unclear.With the further development of embryosac the starch grains in the central cell and antipodal cell disappear in succession,however,the egg cell does not.

在准极核移向胚囊中部前后，不同的功能性细胞有不同的表现，珠孔端的两个"细胞"淀粉粒数量逐渐减少并变得不明显，随着胚囊的进一步发育，其中中央细胞和反足细胞内的淀粉粒相继消失，但卵细胞内却一直保存着。

The quantities of starch grains in the two"cells"in micropyle part of embryosac gradually decrease and become unclear.With the further development of embryosac the starch grains in the central cell and antipodal cell disappear in succession,however,the egg cell does not.

在准极核移向胚囊中部前后，不同的功能性细胞有不同的表现，珠孔端的两个&细胞&淀粉粒数量逐渐减少并变得不明显，随着胚囊的进一步发育，其中中央细胞和反足细胞内的淀粉粒相继消失，但卵细胞内却一直保存着。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

The adipose derived stem cells of inbreds train of rat wereinduced to differentiate into schwann-like cells and the cells were injectedinto the acellular nerve allograff.The proliferation or adhersion of these cells on the graft were observed by inverted microscope or ScanningElectronic Microscope. Activity of cells were detected using MTT. 10mmnerve gap of inbreds train of rats in two group were bridged by tissue-engineered peripheral nerve or autogenous nerve, the effect wereappraised by naked eye, recovery rate of sciatic function index,nerve-electrophysiological, histology, Transmission ElectronMicroscopy and quantitative analysis of recovery rate of myelinated fiberpopulations,diameter of myelinated fiber and thickness of myelin sheat.

按照前述方法培养并诱导近交系大鼠脂肪干细胞向类Sehwann分化，将诱导分化的脂肪干细胞悬液注入已制备的去细胞同种异体神经支架管中，倒置显微镜观察细胞生长情况；扫描电镜下观察细胞在材料上附着情况；四唑盐比色试验测定细胞活性；取两组近交系大鼠，分别用组织工程化神经和自体神经桥接10mm神经缺损，通过大体观察、坐骨神经功能指数恢复率的测定、神经电生理的测定、组织学光镜观察、透射电镜观察、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率的测定等指标评价实验效果。

Results: The medicated serums at these 2 points of time had significant effects in promoting MC cell apoptosis.

两个时间点的药物血清对MC细胞都有显著的促凋亡作用，且能显著降低G0/G1期的MC细胞比例及显著增加G2/M期的MC细胞比例，这可能是其抑制MC细胞增殖及促凋亡的原因之一。

So far, because of the components of AN are very complex, the mechanisms of AN-induced cytotoxicity to cause cell aberrations and oral diseases are not clearly understood. The purpose of this study is trying to investigate the roles of two check point kineses: Chk1/Chk2 in the ANE-induced geno- and cytotoxicity.

但由於槟榔成分过於复杂，对於槟榔成分究竟是如何造成各种细胞变异甚至口腔疾病的详细致病机转目前并不清楚，因此本篇研究的主要目的即是针对细胞内两种细胞周期检查点Chk1/Chk2在槟榔成分所造成的基因与细胞毒性中所扮演的角色进行探讨。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。