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Along with the fluconazole solution density rise,the experimental two kind of strain various glucose density is higher,showe d the glucose consumption are less,takes the logarithmof the medicine de nsity,discovered the logarithm the medicine density and each glucose den sity presents the linear relations;Carries on the analysis comparison to under the fluconazole function two kind of strain linear relations,disc overed the relations of the two strains has the nonuniformity.3 Compare the fluconazole induction reaiatance SC5314 strain and sens itive strain compares,its difference gene expression mainly concentrates in:The code proteinase body and the protein hydroltyic enzyme gene,in the code sugar fat metabolism process is connected the protein gene,the cell cycle correlation gene,the duplication and the translation adjustme nt correlation gene,the stress response correlation gene,the line plast ochondria correlation gene,the cell wall function related gene.4 Candida albicans SC5314 induction resiatance strain was processed b y Xianglian solution,its expression change gene mainly is:Code stress re sponse family protein gene,biomembrane relevant gene,a code proteinase body gene race,code cell cycle related protein gene,duplication and tra nslation adjustment related protein gene.5 The clinical reaiatance strain Candida albicans was processed by Xi anglian solution,its expression change gene mainly is:Codes the hot sho ck protein gene,the serine/threonine protein activating enzyme gene,the proteinase body family gene,the regulation copies and translates the ge ne.

随着氟康唑药液的浓度上升,试验的两种菌株各孔葡萄糖浓度越高,说明葡萄糖消耗越少,经过药物浓度取对数后进行分析,发现取对数后的药物浓度和每孔中葡萄糖浓度者呈现线性关系;对氟康唑作用下的两种菌株的线性关系进行分析比较,发现对两种菌株作用具有不一致性。3氟康唑诱导的耐药SC5314菌株与诱导前的敏感株相比,其差异基因表达主要集中在:编码蛋白酶体及蛋白水解酶的基因,编码糖脂代谢过程中相关蛋白的基因,细胞周期相关基因,转录及翻译调节相关基因,应激反应相关基因,线粒体相关基因,细胞壁功能相关基因。4白念珠菌SC5314诱导耐药株经香莲外洗液作用后,其表达变化的基因主要是:编码应激反应家族蛋白的基因,生物膜相关性基因,编码蛋白酶体基因一族,编码细胞周期相关蛋白基因,转录及翻译调节的相关蛋白基因。5白念珠菌临床耐药菌株经香莲外洗液作用后,其表达变化的基因主要是:编码热休克蛋白基因,丝氨酸/苏氨酸蛋白激酶基因,蛋白酶体家族基因,调控转录及翻译基因。

Numbers of secretory granules, oplasmic reticulums, mitochondrias in ACTH cells all changed distinctly. GTH cytoplast were full of big and small, high density electron secretory granules.STH cells were in activity synthesis and secretion, secretory granules were from fusion to release. TSH cells cytoplast evacuated secretory granules, electron density decreased and vacuofes appeared.

ACTH细胞分泌物颗粒数量、内质网及线粒体均发生明显变化;GTH细胞胞质中充满电子密度较高的大、小两种分泌颗粒;STH细胞处于活跃的合成和分泌状态,分泌颗粒呈现出由融合到释放的状态;TSH细胞胞质中分泌颗粒排空,且电子密度降低,出现大空泡。

PFHT were predominantly composed of epithelioid cells in 11 cases, spindle cells in 1, and an even admixture of spindle and epithelioid cells in 6 cases.

这18例PFHT中的11例主要由上皮样细胞组成,1例由梭形细胞组成,另外6例由以上两种细胞等量混合而成。

The heterogenic C57BL/6J splenocytes disappeared at 1 day after transfusion, but the syngeneic BALB/c splenocytes could survive for a long time in the peripheral blood of BALB/c mice. The fluorescence intensity was not decreased in two kinds of splenocytes. The results demonstrated that CFSE-labeling with difference concentration is reliable, stable and convenient for spleen lymphocytes tracing in vivo.

异基因的C57BL/6J脾细胞在输注1 d后就快速消失,而同基因BALB/c脾细胞能够在BALB/c小鼠外周血中长期存在,但是两种细胞荧光强度均未见降低,说明荧光染料CFSE差异浓度标记活细胞进行体内示踪是一种稳定的示踪方法。

The macronuclear and mitochondria DNA between the resting and the vegetative cell of Euplotes encysticus were comparatively analyzed by using RAPD technique in order to explore the acting feature of two genetic systems in the resting cell of ciliates.

为了探索纤毛虫休眠细胞中两套遗传系统的作用特征,对包囊游仆虫休眠细胞与营养细胞大核DNA和线粒体DNA进行了RAPD比较。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度,然后每个角膜被分成两半,共48个半片角膜,再分成4组,每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数;12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变;12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性;12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

The results showed that: at metaphase I, the homoeologous chromosome pairing among different F1 hybrids ranged from 2.0 to 11.4 bi-valents formed by homoeologous chromosomes per pollen mother cell, and very few multivalents, and even very few bivalents were formed by two chromosomes within one genome rather than homoeologous chromosomes in some PMCs; at anaphase I, all biva-lents were disjoined and most univalents were divided.

结果表明在中期I阶段,这些杂种一代的近缘染色体联会变化很大,每个花粉母细胞中二价体形成的数目从平均2个到11.4个不等,甚至在某些花粉母细胞中,还发现极少的多价体和非部分同源染色体所形成的单基因组内二价体;在后期I时,所有的二价体分离,同时多数单价体也分离,分离的二价体和分离的单价体都移向两极,从而形成两组染色体;因为这时完整花粉母细胞中分离的二价体在两组染色体中总是对应出现,从而根据半二价体上染色体重组的位置可以分析在二价体的四分体时期发生在非姊妹染色体之间的多种染色体交换类型,如单交换、三线双交换、四线双交换、四线三交换和四线多交换。

Results: in contrast with model group, SSM can (1) increase the survival rate of ARF in rats, cut down Scr and BUN at 24hr, improve renal function, slightly surpass Vp in its therapeutic effect;(2) mitigate the extent of renal pathologicchanges, decrease the numbers of renal tubule necroses and casts;(3) protect renal ultrastructure such as microvilli, mitochondria, endoplasmic reticulum of TEC, podocytic process of glomerulus epithelial cell,endothelial cell etc, better than Vp;(4) enhance SOD activity, lower MDA contents in renal cortex;(5) enhance NO contents in serum, reduce ET levels in plasma;(6) evidently cut down TNF- a contents in serum, superior to Vp;(7) antagonize calcium overload in TEC;(8) alleviate renal pathological changes in ARF metaphase (at 3d), advance regeneration and recovery of TEC, restore renal structure in ARF convalescence (at 5d), and better than Vp;(9) increase the expression of TEC PCNA and prepro EGFmRNA in ARF metaphase.

结果:与模型组相比,SSM能(1)提高ARF大鼠的存活率,降低24hr时Scr及BUN,改善肾功能,其疗效略优于维拉帕米;(2)减轻ARF肾脏病变程度,减少24hr肾小管坏死数及管腔内管型数;(3)对肾脏的超微结构如肾小管上皮细胞微绒毛、线粒体、内质网、肾小球上皮细胞足突、内皮细胞等形态结构均有一定的保护作用,其疗效略优于维拉帕米;(4)提高肾皮质SOD活性,降低MDA含量;(5)提高血清NO水平,同时降低血浆ET含量;(6)显著降低血清TNF-α的水平,疗效明显优于维拉帕米;(7)拮抗小管上皮细胞[Ca~(2+)]_i的升高;(8)使ARF中期(第3d时)肾组织病变较同期其它两组明显减轻,并使肾小管细胞再生修复提前,至ARF恢复期(第5d时)肾组织结构恢复重建良好;作用明显优于维拉帕米;(9)促进ARF中期肾小管上皮细胞PCNA的表达及EGF前体mRNA的合成,从而增加EGF的合成释放,而维拉帕米无此作用。

Thus, initially the cages had two cells, but when a random cage cell superposes another cage cell, those cages are merged, so we could have 3-pieces and 4-pieces cages.

因此,在最初的笼子里有两个细胞,但是当一个随机笼笼细胞重合同另一个细胞,这些笼子合并,所以我们可以有3件和4件笼。

Aromatase protein and its mRNA were abundant in the forebrain, midbrain, spinal cord, wheel organ and Hatschek's pit, but not abundant in the hindbrain or early ovary and testis. No aromatase expression was detected in both irregularshaped cells and ciliated mucous cells in the Hatschek's pit or in the mature ovary and testis. Aromatase immunoreactive material was distributed in the cytoplasm with negative nuclei.

芳香化酶蛋白和转录物在前脑、中脑、脊髓、轮器和哈氏窝十分丰富,而后脑、早期卵巢和精巢不够丰富;没有芳香化酶表达的部位是哈氏窝另两种细胞(不规则形细胞和带纤毛粘液细胞)以及成熟卵巢和精巢;芳香化酶免疫活性物质分布在胞质,核为阴性。

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The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了,排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意,我建议我们在价格上大家各让一半。

After an hour and no pup, look for continued contractions and arching of the back with no pup as a sign of trouble.

一个小时后,并没有任何的PUP ,寻找继续收缩和拱的背面没有任何的PUP作为一个注册的麻烦。