两细胞的

Hyperthermia was applied to the latter,41.5 degrees Centi grade, 1 h, after having received same irradiation. The cel surviving fractions were determined by clonogenic assay and the parameters of the multi-target/single-hit model, Do, D9, as well as those of the linear-quadratic model, ratio of alpha and beta, were elicited.

集落形成率实验检测两组细胞在不同情况下的细胞存活分数，分别根据多靶单击模型和线形二次模型拟合绘制细胞存活曲线，求出 D0、Dq及α/β比等细胞存活曲线参数，并获得照射2 Gy时的存活分数SF2。

Cell cycle and apoptosis are two curial events in the mammalian cell.

细胞周期和细胞凋亡是哺乳动物细胞生命活动的两大关键事件。

The SGC-7901GA cells and control cells SGC-7901Neo were cultured in vitro,and then were respectively injected into the endermic tissue of 6-weeks-old BALB/C node mice to create the animal models of tumor. The neoplasia and growth of tumor were observed. Use MTT method to detect the difference of growth capability of two kinds of cells in vitro.

先把SGC-7901~细胞和空白对照组细胞SGC-7901~进行体外培养，再把两种胃癌细胞接种于6周龄BALB／C裸鼠皮下，建立胃癌的裸鼠动物模型，观察裸鼠成瘤情况。

The notable proliferation was not observed by eyes in the local of injection. The infiltration of inflammation cells and mild proliferation of fibrocyte around dura mater was observed by HE stained in 4 and 8 weeks after injection. Infiltration and exudation of inflammation cells was observed by HE stained in epidural nerve root. Compared with group A, no changes of group B, C and D were observed under specific stained. Proliferation of type Ⅱ collagen fibers around dura mater was seen under immunohistochemical stained in 4 and 8 weeks after injection. There is no significant demyelination changes under LFB stained. The thickness and shape of the myelin sheath in epidural nerve root was not regular under transmission electronic microscopy in 4 and 8 weeks after injection. Fibroblast was also seen there. In nerve endometrium, macrophage could be seen under TEM, myelinated nerve fiber changed significantly, but nonmyelinated nerve fiber changed mildly. When 8 weeks, the changes of group D is smaller than the group B and C.

给药局部肉眼观察未见明显的纤维组织增生；HE染色可见B、C、D三组给药后四周及八周时硬膜内外均有炎细胞浸润，纤维细胞轻度增生，硬膜外神经根内有炎细胞浸润及炎性渗出；特殊染色B、C、D三组同A组相比未见有脊髓及神经根的改变；免疫组化染色，给药后四周及八周时，硬膜内外均有Ⅱ型胶原纤维增生；固兰染色B、C、D三组未见有明显脱髓鞘改变，与A组相比无明显异常改变；电镜观察B、C、D三组在给药后的四周及八周时，表现为硬膜外神经根内髓鞘厚薄不一，形状不规则，可见成纤维细胞，神经内膜中可见有巨噬细胞；粗大的有髓神经纤维变化明显，无髓神经纤维受累较轻；八周时电镜下D组改变较B、C两组为轻。

The main components of every ommatidium of compound eye were completed in the larval stages. An ommatidium composed of cornea, four corneagenous cells, four cone cells and retinular cells. The sensitization system of an ommatidium included 11 retinular cells, of which four located at the distal part of the ommatidium; seven formed the proximal main part of the retina. Their structures are different.

小眼的感光系统由11个小网膜细胞组成，且与成体一样，11个小网膜细胞已经分化成4+7两层，其中4个小网膜细胞构成了感光部分的远端，7个小网膜细胞构成感光系统的近端主体，远端和近端的小网膜细胞在结构上也有明显差异。

The expression of CD59 on the cell membrane was tested by cell immunohistochemistry and flow cytometry. Results pALTER plasmid containing CD59 was cut with restriction enzymes and a 496 bp fragment obtained by electrophoresis, which was complete conformity with the insert.

将两种含有不同突变体的人CD59全长cDNA序列重组pALTER质粒，应用阳离子脂质体导入法与pcDNA共转染CHO细胞，用G418筛选阳性克隆，应用细胞免疫组化及流式细胞仪检测CD59在CHO细胞表面的表达。

The first 0.1%collagenaseⅣ 0.1% hyaluronidase for 20 min and than 0.25% trypsin 0.04% EDTA 5μg/mL DNaseⅠfor 5 min,its enough to obtain germ cells and have optimal cell viability. 8.4±2.6×106 cells per gram testis were isolated and there was(19.7±5.3)% of PGP9.5 spermatogonium and (23.8±3.6)% of c-kit spermatogonium.

采用两步酶消化法，0.1%胶原酶和0.1%透明质酸酶消化20 min，0.25%胰酶 0.04% EDTA 5μg/mL DNaseⅠ处理5 min，就可以使睾丸生殖细胞充分分离处出来，每克睾丸组织克获得8.4±2.6×106个细胞，其中PGP9.5 精原细胞占(19.7±5.3)%，c-kit 精原细胞占总数的(23.8±3.6)%。

There are two types of maturation according to our observation.One is nucleocapsids obtained its tegument in the nucleus and enveloped from the inner nuclear membrane.Another is nucleocapsids entered cytoplasm through nuclear membrane,then obtained their tegument in the cytoplasm,enveloped from the plasma membrane,finally released by necrosis,exocytosis or other ways.

病毒成熟有两种方式：一为细胞核内核衣壳在核内获得皮层，通过核内膜获得囊膜成为成熟病毒；二为核内核衣壳通过内外核膜进入胞浆，核内和胞浆内的核衣壳在细胞浆中获得皮层，然后在各种质膜上获得囊膜，最后成熟病毒通过细胞破裂或其他方式释放到细胞外。

Embryonal carcinoma and neuroblastoma are both formed during embryonal development, retinoic acid can induce them to differentiate into neurons.

胚胎瘤和神经母细胞瘤都是胚胎发育期间形成的肿瘤，维甲酸可以诱导这两种肿瘤细胞向神经元方向分化，在研究神经元分化机理时作为细胞模型得以广泛应用。

The recombinant viruses, with the gene of reference RV strain VP2, field strain T114 VP6 and T73 G1-serotype VP7, respectively, co-infected Sf9 cell at a multiplicity of infection of 0.5. Harvested the supernatants of culture when the cells were lysed fully (about 7-10 days), or harvested cells before cell lysates (about 4-5 days after infection) and then treated the cells with lysing solution. The supernatants of and cell lysates were ultracentrifuged twice with 40% sucrose cushion at 93 000g, and the sediment products were harvested with TNC buffer. The purified VLPs samples were separated by SDS-PAGE, and then stained by Coomassie blue and silver nitrate and detected by Western blot to analyze the composition of VP2, VP6 and VP7 and their specificity. The morphologic structure of recombinant VLPs was detected by EM.

含标准株VP2、地方株T114 VP6和地方株T73 G1型VP7蛋白编码基因的重组病毒以0.5 MOI共感染Sf9细胞制备2／6／7病毒样颗粒，待细胞完全裂解后收获培养基上清或感染后5天细胞裂解前收获细胞并用裂解液裂解，培养基上清和细胞裂解液样品两次40％蔗糖垫93000g超速离心，沉淀样品经SDS-PAGE胶分离，用考马氏亮兰和硝酸银染色以及Western blot检测样品中VP2、VP6和VP7蛋白的组成以及特异性，电镜检测重组病毒样颗粒的形态结构。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。