两端

Based on the study, it is very convenient for making a deep research of the foreign gene expression under the regulation of CSN2 flanking region.

为进一步利用 CSN2 质粒两端的调控序列，插入新的基因，研究其表达功能，或者进行乳腺生物反应器的研究奠定了基础。

Emblica have many different forms and most of them have tails and their perforation plates divide into two types:(1)there is one simple perforation plate at each end of the vessel elements and (2) there is one simple perforation plate at one end of the vessel elements and two simple perforation plate at the other end;(3)a few vessel elements have special inclusions;(4)the intervascular pits are alternate pits;(5)the pits between the vessels and rays are mixed pits ,pits with transverse gashes and scalariform pits.

结果发现，余甘子次生木质部导管分子中存在着许多不同的样式，导管分子大多数具尾；其穿孔板存在着两种类型：(1)两端均为1个单穿孔板；(2)一端为1个单穿孔板；另一端为2个单穿孔板；(3)极少数的导管分子具有特殊的内含物；(4)管间纹孔式为互列纹孔式；(5)导管射线间纹孔式为混合型纹孔与横列刻痕状纹孔以及梯状穿孔。

The biomechanical tests showed that two kinds of artificial bones had not significant difference on compressive strength and Young\'s modulus(P＞0.05),while the flexural strength of nano-nacre artificial bone was less than the control group(P＜0.05).3.The results of CCK-8 showed that the difference were not significant in each group,the proliferation of osteoblast reached the peak at the 5th day;7 days after being co-cultured,the total protein content of study group was higher than control group and blank group(P＜0.05),while the difference between control group and blank group was not significantP＞0.05The difference of alkaline phosphatase activities among three groups was not significant(P＞0.05The SEM view showed that osteoblast attached and grew well in two kinds of artificial bone.4.X-ray photography showed that two kinds of powder started to degrade in 2 weeks;this phenomenon became more appear in 4 weeks,nano-nacre powder degraded faster than micron-nacre powder,while the hole shadow was easy to be found;in 8 weeks,all the femoral holes recovered and returned to normal bone mineral density in all groups.Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone grew fastest around the bone defect area in study group,while most slowly in blank groupP＜0.05 SEM(scanning electron microscope observation showed that nano-nacre powder degraded more quickly.The same result can be found through the demineralized sections morphometric analysis,and both of the composite artificial bones made from those two kinds of nacre powder had the good connection with the adjacent tissue in rats body without apparent inflammatory response.5.X-ray photography showed that rabbit\'s bone defects healed faster in study group since NNAB implanted than in control group since MNAB implanted.At 24 weeks after operation,bone density in radial defects had nearly accessed to the normal area,while lower in control group,and turned up nonunion in blank group;The checking of BMD showed that results in study group were higher than those in control group at 8,16 and 24 week(P＜0.05), and the difference between the BMD values in study group at 24 week and those in blank group was not significant(P＞0.05).The gross specimens showed satisfactory histocompatibility both in study group and in control group,with bone tissue growing from two sides into the center of implanted materials; Normal slices in HE stain and hard tissue grinding slices in Stevenel\'s blue/Van Geison\'s picro-fuchsin stain showed that the bone growth tendency was better in study group than that in control group,and the medullary cavity had been penetrated to the implanted materials in study group at 24 week;Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone in both groups grew fastest 8 weeks after surgery,while slow down at 16 week.

纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨分别与成骨细胞共培养后，其各时间点CCK-8法检测值与空白对照无显著差异(P＞0.05)，成骨细胞均在第5天达到增殖高峰期；培养7天后，实验组细胞蛋白含量高于对照组及空白组(P＜0.05)，后两者之间则无显著差异P＞0.05碱性磷酸酶活性在三组间均无显著差异(P＞0.05电镜下可见成骨细胞在两种人工骨上都有良好生长贴附能力。4.X-ray显示两种粉体在大鼠股骨骨洞植入第2周时都开始出现了降解，第4周时更为明显，纳米珍珠层粉较之微米珍珠层粉降解更快，而空白对照组骨洞阴影仍可见，至8周时，则所有组骨洞均己闭合修复，X-ray下已不可见原钻孔痕迹，恢复正常骨质密度；硬组织磨片四环素荧光双标记结果显示纳米珍珠层粉植入组较其余两组在骨缺损区周围新骨生长速度更快，空白组速度最慢P＜0.05电镜观察及常规脱钙切片亦可见到纳米粉体降解较快；由以上两种原材料制得的纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨在大鼠体内均与周围组织结合良好，无明显炎症反应。5.X-ray显示纳米珍珠层/消旋聚乳酸复合人工骨植入兔桡骨缺损区后其骨愈合速度较对照组微米珍珠层/消旋聚乳酸复合人工骨植入的快，至植入术后24周，实验组骨缺损区接近正常骨密度，对照组骨缺损区密度较低，空白组则呈现骨不连状态；骨密度测量结果显示术后8周、16周、24周实验组的骨密度值高于对照组(P＜0.05，24周实验组的骨密度值与术前所测得的正常值无显著性差异P＞0.05动物取材大体所见均显示组织相容性良好，骨组织逐渐由植入材料两端向中央生长；常规切片HE染色及硬组织磨片Stevenel\'s blue/Van Geison\'s picro-fuchsin联合染色均可见实验组骨缺损区长势优于对照组，至术后24周，实验组骨髓腔与材料已呈相交通状；硬组织磨片荧光显微镜下观察，两组材料在术后8周处于骨生长最快速时期，16周时速度开始减慢，术后4、8、16周时实验组的新骨生长速度均较对照组的快

The enzyme amplified lanthanide luminescent spectrometry of horseradish peroxidase was developed and applied to the analyse of HRP and tuberculosis. The deconvolution curve method was used for synchronous fluorimetric determination of two component systems of highly overlapped spectra, such as phenol and p-dihydroxybenzene, phenol and o-dihydroxybenzene, and tyrosine and tryptophan. A novel mathematical method, combining Gorry's deconvolution method, was presented to remedy data loosing of SavitzkyGolay's deconvolution method and applied to dealing with fluorescent signal.

建立了辣根过氧化物酶的酶联放大镧系发光法，并应用于结核抗体的测定；成功地应用褶合曲线分析法并进行了改进，结合同步荧光光谱法用于苯酚/对苯二酚、苯酚/邻苯二酚和酪氨酸/色氨酸等光谱重叠较严重的双组份体系的同时测定；结合Gorry提出的卷积法，提出一种解决Savitzky-Golay卷积法中两端信息丢失问题的快捷方法。

In this model, the NAC dimmer localized at the center; The first part (1-62aa) and the third part (114-162aa) surrounded this center and formed heterogenetic association; The UBA domain distributed at two ends.

这个模型的中心为NAC二聚体结构，第一部分(1-62位氨基酸)和第三部分(113-163位氨基酸)围绕这个中心异源聚合在一起，UBA结构域分布在两端。5、PeaT1结构与功能的关系。

A very large sample plug was introduced hydrostatically into the capillary (65 cm×50 μm i.d., effective length of 35 cm) over 80 s at a height difference of 11 cm. The working conditions for CE-FA separation are as follows: operating voltage, 10 kV; running buffer, 67 mmol/L phosphate, pH 7.4. The unbound ketoprofen concentration was directly measured from the height of the frontal peak.

一大体积样品虹吸进样至未涂层毛细管(65 cm×50 μm i.d。，有效长度35 cm)，进样时间80 s，毛细管两端高度差11 cm，工作电压10 kV，运行缓冲液为67 mmol/L磷酸盐缓冲液(pH 7.4)，游离酮洛芬浓度由前沿峰的高度直接测定。

A micronuclear version of the GARP gene was amplified by polymerase chain reaction. The macronuclear DNA molecule carrying the GARP gene is 460 bp long and shows the characteristics of macronuclear chromosomes of hypotrichous ciliates.

大核中该基因的染色体全长460 bp，基因两端具有下毛类纤毛虫大核特有的端粒序列(C4A4C4A4C4A4C4)，开放读框内含有一个TGA(88-99)密码子，在游仆虫中编码为半胱氨酸。

In clinical,we fetched bilateral external jugular vein to make double vascular bridge,which both ends were all trousers-style with one open-ing and repaired iliofemoral and subclavian vein,and made corresponding vascular bridge to carry on shunt of artery and vein.

临床上取双侧颈外静脉并制作成两端均为裤式单开口的双血管桥进行髂-股和锁骨下静脉等修复；制作相应血管桥进行动、静脉转流。

Methods In animals test,we made canine small saphenous or external jugular vein into double vascular bridge, which single and both ends were all trousers-style with one opening and transplanted between iliofemoral artery and vein separately and took as bridge-style blood vessels of artery and vein shunt,and detected the blood flow.

在动物试验中，将犬的小隐或颈外静脉制作成单根和两端均为裤式单开口的双血管桥，分别移植于髂-股动、静脉间和作为动、静脉转流的桥式血管，进行血流量的检测。

Methods In animals test,we made canine small saphenous or external jugular vein into double vascular bridge,which single and both ends were all trousers-style with one opening and transplanted between iliofemoral artery and vein separately and took as bridge-style blood vessels of artery and vein shunt,and detected the blood flow.

目的 寻找适宜和较口径的自体移植血管方法在动物试验中，将犬的小隐或颈外静脉制作成单根和两端均裤式单开口的双血管桥，分别移植于髂-股动、静脉间和作为动、静脉转流的桥式血管，进行血流量的检测。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。