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The results showed that the feeder layers prepared by ICR and Kunming mice had no significant effect on adherence of embryos and proliferation of ICMs. The trypsin, which affected the isolation of ES cells remarkedly, should be used with concentration of 0.125% trypsin+0.02% EDTA for KMSmin.

从实验结果来看,ICR小鼠和昆明小鼠两种来源的饲养层细胞对胚胎贴壁、ICM增殖无影响,消化液作用影响明显,以0.125%trypsin+0.02%EDTA消化10~15min为宜。

I am twenty-five years old and have worked on this job for two years.In order to get a developement in my occupation,I peparing a resing.

我今年25岁,已在目前的职位工作两年,兹为寻找更上一层楼,准备离开此职位。

It is shown that the two strains are gramnegative bacteria, which are short rhabditiform, of sterile gemma, and have a layer of viscous capsule.

结果表明:这两菌株均为革兰氏阴性菌,短杆状,不产芽孢,有一层粘的荚膜。

The nanoparticle structure and nanolayer structure of SiO2 and C in a silicious gangue were found by Atomic Force Microscope.

通过AFM研究,发现硅质煤矸石的两种主要组成SiO2和C具有纳米粒状结构和纳米层状结构。

Removing the parchment, two translucent bluish green coffee beans are revealed, coated with a very thin layer called the silverskin.

消除羊皮纸,两个半透明的蓝色调绿咖啡豆揭示,涂上薄薄的一层非常称为 silverskin 。

The skyline of the Pearl River in the south, together with the skyline of Tianhe Sports Complex and the 88-storey CITIC Plaza in the north forms a wave-like skyline with rhythm, while the skyscraping twin towers on the north bank and the sightseeing TV tower on the south bank form a beautiful riverside scene of two banks and three towers.

珠江岸线天际轮廓线与北部天河体育中心、88层中信广场共同形成有节奏、有韵律的波浪形天际轮廓线。而北岸两幢超高双塔与南岸电视观光塔三足鼎立,两岸三塔成沿江美景。

By core observation and slite analysis, two types of fractures can be identified in reservoirs including macro-fractures and micro-fractures. Macro-fractures refer to tectonic fractures and stylolites, and micro-fractures mainly refer to grain breakage fractures and micro-tectonic fractures.

通过岩心观察、薄片分析等方法研究让纳若尔油田储集层裂缝发育特征,发现油田发育宏观裂缝和微观裂缝两种不同尺度的裂缝,宏观裂缝包括构造裂缝和缝合线,微观裂缝主要是颗粒破裂纹和构造微缝。

The scFv formed inclusion body in E. Coli. 8 mol/L urea was used to solubilize the inclusion body, and the scFv was then purified in two steps , including Ni 2+ chelating affinity chromatography and gel filtration.

大肠杆菌表达的单链抗体包涵体,用8 mol/L尿素裂解,经过Ni离子螯合亲和层析和凝胶过滤两步纯化后,纯度达到97%以上,再通过透析复性除去尿素。

These Thoughts so oppress'd my Mind, that I began to give over my Enterprize, and having haled my Boat into a little Creek on the Shore, I stept out, and sat me down upon a little rising bit of Ground, very pensive and anxious, between Fear and Desire about my Voyage; when as I was musing, I could perceive that the Tide was turn'd, and the Flood come on, upon which my going was for so many Hours impracticable; upon this presently it occurr'd to me, that I should go up to the highest Piece of Ground I could find, and observe, if I could, how the Sets of the Tide, or Currents lay, when the Flood came in, that I might judge whether if I was driven one way out, I might not expect to be driven another way home, with the same Rapidness of the Currents: This Thought was no sooner in my Head, but I cast my Eye upon a little Hill, which sufficiently over-look'd the Sea both ways, and from whence I had a clear view of the Currents, or Sets of the Tide, and which way I was to guide my self in my Return; here I found, that as the Current of the Ebb set out close by the South Point of the Island; so the Current of the Flood set in close by the Shore of the North Side, and that I had nothing to do but to keep to the North of the Island in my Return, and I should do well enough.

我思想压力很大,不得不考虑放弃原定的计划。我把小船拉进沿岸的一条小河里,自己迈步上岸,在一块小小的高地上坐下来沉思。我心情忧郁,心绪不宁。我害怕死,又想前去探个究竟。正当我沉思默想之际,只见潮流起了变化,潮水开始上涨。这样,我一时肯定走不成了。这时,我忽然想到,应该找一个最高的地方,上去观察一下潮水上涨时那两股急流的流向,从中我可以作出判断,万一我被一股急流冲入大海,是否有可能被另一股急流冲回来。我刚想到这一层,就看见附近有一座小山;从山上可以看到左右两边的海面,并对两股急流的流向可以一目了然,从而可以确定我回来时应走哪一个方向。到了山上,我发现那退潮的急流是沿着小岛的南部往外流的,而那涨潮的急流是沿着小岛的北部往里流的。这样,我回来时,小舟只要沿着北部行驶,自然就可以被涨潮的急流带回来。

The damage characteristics of the composite laminates under low velocity impact and the succedent compression were researched.

试验研究了5224/G803和5224/G827两种复合材料层合板低速冲击及冲击后压缩载荷作用下的损伤特征。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。