两层的

It is now well understood that asparagine synthetase is composed of two parts: a wedge-shaped place in the two antiparallel β-overlapping layers near the N-terminus that functions in glutamine hydrolysis; a C-terminus responsible for binding of Mg2+ ATP and aspartate.

现又明确天冬酰胺合成酶是由两部分组成：N基末端的两个反平行式β-折叠片层中的楔形部分是负责水解谷氨酰胺的活性部位；C基末端负责结合Mg2+ATP和天冬氨酸。

The work described in this paper,by adopting a parameterized adaptive meshing image reconstruction method to yield the interface between the two layered phases, aims to improve the existing static mesh based ERT measurement accuracy for a stratified two phase flow within a closed pipe, assuming that the phase interface is of a distinctive nature or its fuzziness in the boundary region omittable.

电阻层析成像技术是一种非常有潜力的两相流/多相流检测技术，可实现对封闭管道或者过程容器设备内部多相组分物质参数的可视化测量，具有非侵入、响应速度快、成本低、安全性能好、适用范围广等优点，适用于两相流/多相流中以液相为连续相的生产过程。

The results showed as follows: 1 Extraction of gelsemium total alkaloids. Total alkaloids were extracted with 95 per cent ethanol. The extract rate was 0.519 per cent and the extract purity were 88.02 per cent and 87.38 per cent; Total alkaloids were extracted with chloroform .The extract rate was 0.416 per cent and the extract purity were 89.02 per cent and 94.52 per cent. 2 Isolation of gelsemium total alkaloids with TLC. Total alkaloids contained five or more alkaloids. The optimal developing agent was chloroform :methanol :ethyl acetate(9:l:0.3),the optimal eluent was methylene chloride:methanol(92:8). 3 Isolation of total alkaloids with column chromatography. Alkaloid A and alkaloid B were obtained, alkaloid B was identified as koumine.

研究结果如下：1、钩吻总碱的提取：用95％的乙醇提取钩吻生物碱提取率为0.519％，其提取物纯度为88.02％、87.35％；用氯仿提取钩吻生物碱提取率为0.416％，其提取物纯度为89.02％、94.52％。2、钩吻总碱的薄层层析分离：总碱中含有5种或5种已上的生物碱；氯仿：甲醇：乙酸乙酯(9：1：0.3)是最佳薄层层析的展开剂；二氯甲烷：甲醇(92：8)是柱层析的最佳洗脱剂。3、钩吻总碱的柱层析分离：共得到两种钩吻生物碱单体成分碱A和碱B，碱B为钩吻素子，碱A可能是钩吻素甲。4、钩吻总碱的急性毒性试验：钩吻总碱小鼠腹腔注射的半数致死量LD_(50是：7.38mg／kg，95％的可信限范围为：6.67mg／kg～7.94mg／kg。

In this paper, thermogram was used to do human face recognition. The thermogram of human face was captured from infrared camera. Image processing technologies were used to preprocess the captured thermogram. Then several features were extracted from the processed thermogram. These features included the distribution of temperature statistics, the perimeter of the face, the length/width ratio of the smallest rectangle which contained the face, and the triangle formed from the three lowest temperature points of the two cheeks and the chin. A three-layer back-propagation neural network was used as the recognition tool.

本文中利用红外线摄影机撷取人之脸部所散发之热分布影像以进行人脸辨识，热影像经过前处理后，再撷取与温度分布有关之相关特徵资料，所撷取之特徵资料包括温度统计分布、脸部边缘长度、包含脸部之最小矩形之长宽比、以及两颊与下巴三个部位之温度最低点之距离，并使用三层架构的倒传递类神经网路作为辨识的工具，辨识的过程包括两个步骤：训练与测试。

We have concluded five key tectonics geologic characteristics of Chinese foreland basins based on the study of the sedimentary infill, tectonics evolution, tectonic dynamics, spatial location distribution and structural deformation geometry.(1) The foreland basin is the superimposition of the compression sedimentary stratigraphic sequence with the extension basin, and the foreland basin includes underlying rift basin and upper dustpan flexure deposited basin.(2) The foreland thrustfold structural deformation has occurred four times since the Protozoic, in the Late Caledonian, Late Hercynian, Indosinan and Late Himalayan, respectively.(3) The foreland basin developed based on the control of two factors that are the basin basement collaged by many small plates or terrains and the rejuvenation of ancient orogenic belt, so the foreland basins or foreland thrusts are located in front of ancient orogenic belt.(4) The foreland basins and the rejuvenation of ancient orogenic belt make up a giant basinmountains system resulting from the distant effect of IndiaEurasia collision, which is located on the outer circle of Tibet Plateau, so the thrust deformation intensity, basin deposition range and coupling between orogenic belt and basin changed from strong to weak as from interior to outer circle of Tibet Plateau.(5) There are many structural styles of the thrust and fold belt in different basins, such as brittle behavior faultrelated fold, ductile behavior basementinvolved structure, basementinvolved faultrelated fold, and salt structure.

在综述前人认识的基础上，笔者通过对中国前陆盆地的构造演化历程、沉积充填特征、构造成因及其空间分布规律、构造变形特征等的研究，提出了中国前陆盆地构造地质发育的5个主要特征：(1)两种不同性质的原型盆地发生正反转的叠合性，即挤压构造下作为&本体&的前陆层序与拉张构造下作为&基础&的裂谷、断陷盆地之间的叠置；(2)显生宙以来中国大陆先后发生了4期前陆冲断构造演化的多期性，它们分别是加里东晚期、海西晚期、印支期和喜马拉雅晚期；(3)基于小克拉通基底拼贴后在造山带前缘复活再生的继承性，即统一拼合大陆内部的构造变形导致古造山带的复活，在古造山带边缘发育新生代前陆盆地或前陆冲断带；(4)在空间分布上受环青藏高原巨型盆山体系控制发生陆内变形的系统性，在环青藏高原巨型盆山体系内构造变形强度、盆地沉降幅度、盆山耦合程度等从内环向外环依次降低，从古造山带向克拉通方向构造变形强度依次降低，构造变形样式逐渐简单、构造变形时间依次变新；(5)前陆冲断带的构造样式由于受边界力学条件和沉积地层介质作用而具有多变性，存在沉积盖层内脆性变形的断层相关褶皱、造山带前缘韧性变形的基底卷入构造、与走滑构造相伴生的基底卷入的断层相关褶皱、盆地内部塑性变形的盐构造。

Two factors, refractive index and reflective area related with the retroreflective capability are discussed.

从理论和实验两方面分析了玻璃微珠的回向反射特性，主要讨论了玻璃微珠的折射率和其反射层面积这两个因素对回向反射性能的影响。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

Cold water belts between well-mixed and stratified regions are simulated by the model.

模拟结果表明，在层化与混合区之间存在冷水带，它位于潮锋的混合侧，并与表底温度差为1～2℃的等值线位置相吻合，这一冷水带主要是由以下两种机制来维持的：1）锋面产生的截锋面方向上的环流，把位于层化侧下层的低温水卷挟到海表层；2）风和潮共同产生的混合作用。

Just as there are two types of network service, connection-oriented and connectionless, there are also two types of transport service.

如同网络层有两种不同类型的服务一样，传输服务的类型也有两种。

Thirdly, by con-utilaziont of many methods such as exploitation and geology, dynamic producing process monitoring, water invasion behavior, core analyis and numerical simulation, 10 kinds of reservoir scale remaining oil distribution pattern in the steam huff and puff super heavy crude oil reservoir was put forward. Also, remainling oil distribution in the sand bed scale and sublayer scale were pointed out.Fourthly, Microcosmic displacement experiment was conducted for hot water driving in 100℃and 120℃. and steam drive at 170℃condition. Microcosmic remaining oil distribution characteristics were summarized. Remaining oil microcosmic distribution by steam drive at 170℃is different from that at 100℃, 120℃hot water driving. After the steam drive, there are three types of remaining oil distribute regular pattern, whic is impacted by many affecting factors like pore configuration, wettability and interfacial tension.

分析了砂层组规模和小层规模的纵向和平面剩余油分布规律。4、通过微观驱替实验，研究了100℃和120℃的热水驱及170℃蒸汽驱条件下剩余油的微观分布形态。170℃条件下剩余油微观分布形态同时受孔隙结构、润湿性及界面张力多种因素的影响，主要有三种形式：在油、汽、水三相共存的孔隙中，剩余油分布于汽、水之间，为薄膜状和弯月状；在只有油、汽两相共存的孔隙中，大孔道中主要是蒸汽，剩余油在细小的孔喉和孔隙角隅处以段塞或不规则状态存在；由于高温水蒸汽对原油具有萃取作用，剩余油以一种细小的珠滴状存在于孔隙表面。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。