两受精卵的

One of two sperm get into an archegonium and fused with egg. The nucleus of fertilized egg began to divide, and the walls were formed after the third division. The eggs without fertilization divided two times and then disappeared.

雄配子体产生的两个精子中的一个进入颈卵器与卵细胞结合形成受精卵，受精卵核到第3次分裂形成细胞壁，没有受精的卵细胞还要进行两次有丝分裂后才消失。

High culturing temperature results in short interval between fertilization and eclosion, whereas more days are required at low temperature. The culturing temperature has a significant influence upon the numbers of P.

在连续60天的观察中，发现同一天的受精卵，在实验室中有两个羽化高峰，间隔呈现似半月周期的现象，两峰的间隔会受全期平均温度的影响，高温两峰的间隔短，低温则较长。

Recombinant expression vector k14/c-myc-paav was constructed bearing the mouse c-Myc cDNA derived by the skin specific human keratin 14 promoter, then the linear vector was microinjected into the two pronucleus of the C57BL/6 fertilized eggs to produce transgenic mice.256 microinjected eggs were transferred, 6 of the foster mothers were pregnant and 44 adult mice were produced, two of them were positive transgenic mice confirmed by PCR and Southern blot analysis, transgenic rate was about 4%.

以CMV-PAAV载体为基础载体，以皮肤特异人K14（人角蛋白14）基因启动子替换原质粒中的CMV启动子后，在其多克隆位点区依次插入鼠c-Myc基因的cDNA序列和gfp基因的编码序列，构建了高效表达c-Myc基因的真核表达载体。通过双原核显微注射技术，将线性化的载体DNA注入到C57BL/6小鼠受精卵的两个原核中，然后将注射后状态良好的胚胎移植到代孕鼠的输卵管中，共移植了256枚受精卵，6只受体怀孕，最终获得44只成年小鼠。

This suggests that CaHIRA plays a role in the early embryo development and the late stages. The different expression characters of hira gene in these fish species may be related to the different reproduction models. The research on Drosophila has indicated hira gene related to sperm nucleus decondensation during fertilization. The high expression of hira gene before fertilization is prepared for the sperm nucleus decondensation and the formation of male prenucleus because colored crucian carp is bisexual reproduction. However, the sperm nucleus is not decondensated during the fertilization and is only to activate egg nucleus in gynogenetic gibel carp.

在彩鲫中，从未受精卵到胚胎发育的各个时期均可检测到表达，但表达量发生明显变化，受精后表达量迅速下降，直到原肠胚期表达量有所上升，并一直保持到出苗。hira基因在两亲缘种成熟卵子受精前后表达情况不同，可能与两种鱼不同的生殖方式有关。

Methods Using the means of culturing amphicytula in vitro to 2-cell and tubal insufflations to collect the 2-cell amphicytulas, then did embryo freezing, and compared the effect of superovulation of FCB mouse which live for 4~6weeks, 6~8weeks.

对4~6周、6~8周、8~10周FVB小鼠进行超数排卵效果的比较及采用体外培养受精卵至2~cell法和输卵管吹卵法两种不同方法收集2~cell受精卵进行胚胎冷冻。

To give birth to twins .fraternal twins are biovular.

二卵双胞胎是源于两个分开的受精卵的。

A pair of pliers .fraternal twins are biovular.

二卵双胞胎是源于两个分开的受精卵的。

A close fraternal tie.fraternal twins are biovular.

二卵双胞胎是源于两个分开的受精卵的。

Fraternal twins are biovular.

二卵双胞胎是源于两个分开的受精卵的。

Oocyte samples from one group were collected to detect the presence and integration of HBV DNA within cells and chromosomes using PCR, Southern blot, dot hybridization and fluorescence in situ hybridization. The female animals from another group were mated with their normal males, respectively. Their zygotes, 2-cell embryos were collected to detect the integration of HBV DNA in the female pronuclei of zygotes and the replication and expression of HBV genes in the 2-cell embryos using FISH, RT-PCR and immunofluoresence assay.(1) PCR detected positive bands in the tested oocyte samples fromgoldon hamster and mice. Southern blot revealed clear hybridization signals in PCR products.

研究用金黄地鼠和小鼠建立实验动物模型：将卵巢内注射HBV DNA的实验动物分成两组，一组注射后进行超排卵，收集卵巢和输卵管的卵母细胞，用PCR、Southern杂交，斑点杂交和荧光原位杂交(fluorescence in situ hybridization、FISH)检测HBV在卵母细胞内的存在和染色体上的整合；另一组超排雌鼠与正常雄鼠合笼，收集受精卵和2-细胞胚，用FISH、RT-PCR和免疫荧光检测技术分别研究HBV基因在受精卵雌原核上的整合以及在2-细胞胚中的复制与表达。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。