丝间质

ER took part in cell wall material deposition during endosperm cell construction. ER rounded up matrix forming endocytic vacuole. ER cisterna swelling and accumulating starch developed into amyloplast. ER took part in protein synthesis and deposition, played a core role in protein body formation. ER swelled at the end to form many vesicles. ER was associated with the plasmodesmata, helped transporting nutrients at the development and differentiation stage.

内质网参与胚乳细胞构建中细胞壁物质的积累；内质网包裹基质形成吞噬体，为胚乳的发育提供营养；内质网槽库膨大，积累淀粉转变成淀粉质体；内质网参与蛋白质的合成与积累，在蛋白体的形成中处于核心地位；内质网末端节状膨胀，形成潴泡；灌浆高峰期内质网常与胞间连丝相连，有助于物质的运输。

By the furnace body, the stainless steel crucible, electrically operated toward the multiple swinging material frame, stainless steel fast Leng Bao, the heating unit and control the warm cabinet to be composed automatically, may use the different resolver, carries on components quenching, the tempering heating, the isothermal quench to cool, the liquid thermochemical treatment and so on many kinds of crafts, the furnace body becomes by the section and the steel plate welding, the stove surface uses δ=14mm the board to make, the nitre salt furnace lining uses the ultra light quality high strength 0.6g/cm3 micro bead refractory brick, clamps the stratification aluminum silicate textile fiber, the furnace lining and the furnace shell fills the heat preservation inflation heat preservation powder, the entire structure for the standard energy conservation furnace lining, the nitre salt stove's heating element uses the thick diameter 0Cr25Al5 high temperature resistance wire to circle spiral-shaped according to the average temperature cloth silk law,Places in the furnace lining puts on the silk brick, puts the silk brick to use the front part to go up 20° against falls the silk brick, the resistance wire is not easy to drop out.

由炉体、不锈钢坩锅、电动往复式摆动料架、不锈钢速冷包、电热元件和自动控温柜组成，可以使用不同溶剂，进行零件淬火、回火加热、等温淬火冷却，液体化学热处理等多种工艺，炉体由型钢和钢板焊接而成，炉面采用δ=14mm板制成，硝盐炉衬采用超轻质高强度0.6g/cm3微珠耐火砖，夹层置硅酸铝纤维，炉衬与炉壳间填充保温膨胀保温粉，整个结构为标准型节能炉衬，硝盐炉的发热元件采用较粗直径0Cr25Al5高温电阻丝绕成螺旋状按照均温布丝法，安置在炉衬的搁丝砖上，搁丝砖采用前部上翘20°防掉丝砖，电阻丝不易掉出。

This study investigated the mechanisms that induce EMT and the significance of extracellular signal-regulated kinase cascade in this course in vitro.

本文旨在研究酸性成纤维细胞因子对上皮-间质转分化的影响，探讨有丝分裂原激动蛋白激酶的ERK信号传导途径在此过程中的作用，为肾小管-间质纤维化的防治提供理论依据。

In addition, glomerular enlargement, hypertrophy, and glomerular mesangial deposits lead to a progressive glomerulosclerosis and eventual nephron loss.

因此，肾丝球体的扩大，肥大，和肾丝球间质沈积，导致累进式肾丝球体硬化，以及最后一个肾脏小过滤器丧失。

In cells with active secretion, enzyme reaction product was present in large quantities on the plasma membrane, vacuo le membrane, plasmodesma and in the endoplasmic reticulum, plastid lamellae,and only in small quantities in the mitochondria and small vesicles.

分泌活动旺盛的细胞中，质膜、内质网、质体的内部片层、胞间连丝以及多数大液泡的膜上面都有大量ATP 酶活性反应产物，线粒体和小泡上只有少量酶活性反应产物。

2Myocardial ultrastructure was normal in the control group,myoneme arranged disordered,the structure of typical sarcomere disappeared, mitochondrium intumesced,interstitial substance fiber hyperplasy,capillary vessel endothelial cell swelled in DG group.Myocardial ultrastructure improved slightly in rats of SM and LM groups. 3NF-κB p65 and COX-2 had a small quantity to present in the myocardium tissue of control group,while the quantity increased obviously in the diabetic group.The level of it in the group of treatment was significant lower than that in the diabetic group.There was significant difference between the two groups. Conclusion Metformin can protect against the heart injury in diabetic.

结果 1与对照组比较，DG组体重明显减轻，血糖、心脏质量指数、INS、TG、T-CH及FFA明显升高(P＜0.01)；应用 MT治疗后体重无明显增加，INS有所下降，心脏质量指数、血糖、TG及FFA明显降低(P＜0.01)；DG组心肌组织MDA明显升高，SOD明显降低(P＜0.01)，MT治疗后上述指标明显改善；2超微结构观察单纯糖尿病组肌丝结构明显紊乱，典型肌小节结构消失，线粒体肿胀、间质纤维增生，微血管内皮细胞肿胀等表现，而SM及LM组可见组织结构有所改善；3对照组心肌NF-κB p65及COX-2只有少量表达，单纯糖尿病组表达量明显增多(P＜0.01)，MT治疗后表达量明显减少(P＜0.01)。

This paper summarizes the recent progress in studies on the plasmodesmatal diversity, the regulation of plasmodesmal SEL, the plasmodesmal macromolecule trafficking and the symplasmic domains in relation to plant morphogenesis and dormancy, etc.

本文仅就胞间连丝结构的多样性；胞间通道的调节因子；大分子蛋白质和核酸的胞间运输；胞间连丝阻断和共质体分区的形成及其与形态发生、休眠和抗逆性的关系等几个方面的新进展做一个简要的综述，借此例证胞间连丝在植物生命活动中的重要意义。

This observation provided a strong support to the point that most leaves export the most of assimilates in the light time. Plasmodesmal densities between SE/CC, CC/PP, PP/PP and PP/BSC (bundle-sheath cell) decreased in weak light. Plasmodesmata were observed between CC/SE (nacreous-walled sieve element), PP/BSC in branch veins in normal light intensity, but not in weak light. Thus apoplasmic pathway may be the main mode of transport of assimilates in weak light, however symplasmic pathway may be the main mode of transport of assimilates in normal light intensity.

在筛管/伴胞、伴胞/韧皮薄壁细胞、韧皮薄壁细胞/韧皮薄壁细胞和韧皮薄壁细胞/维管束鞘细胞之间的胞间连丝密度都在弱光条件下下降，在正常光照强度下支脉筛管/伴胞和韧皮薄壁细胞/维管束鞘细胞之间可以观察到胞间连丝，而在弱光下几乎观察不到胞间连丝的存在，所以同化物的运输在弱光条件下可能以质外体运输为主，而在正常光照强度下，共质体运输可能是主要的运输方式。

Methods Marrow-derived mesenchymal stem cells were isolated and purified from mouse femoral bone and shinbones using differential adherent methods. Cells at the third passage were induced by 20% FBS in conditioned medium, conditioned medium alone, 20% FBS or 10% FBS alone respectively. Mouse aortic smooth muscle cells were cultured as the positive control. Levels of mRNA and protein expression of myoeardin and several smooth muscle cells marker genes were determined by immumofluorescenee, RT-PCR and Western blot before and 3, 7, 10, 14d after the induction. The presence of smooth muscle myofilaments was detected by using transmission electron microscope.

采用伞骨髓贴壁法分离骨髓问充质干细胞，CM联合20%FBS诱导骨髓间充质干细胞，同时设立持续10%FBS、单20%FBS及单CM诱导下的骨髓间充质十细胞对照组和SMC阳性对照组，分别于诱导前及诱导3、7、10和14 d时观察细胞形态的变化，并在相应的时间点用免疫荧光法、逆转录聚合酶链反应法、Western blot半定量分析法榆测myocardin以及SMC表面各种标志基因的表达变化，用透射电镜检测诱导后细胞内肌丝存在以此来证实诱导分化成功。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。