丝状真菌

Subsequently,germ elongation ceases and the tip of the germ tube swells to form an appressorium surrounded by a thick melanized cell wall.

稻瘟病菌是引起水稻重要病害稻瘟病的病原菌，也是研究丝状真菌的重要模式生物。

It is very difficult to extract active RNA from Aspergillus niger because of the existance of complex cell wall and endo-Rnase activity.

由于复杂的细胞壁和内源酶活性的存在，所以要从丝状真菌中提取和纯化出有生物活性的RNA比较困难。

To study and know well the rudimentary knowledge and the basic techniques of modern Microbiology are demanded for Graduate students. The experiments including the principle and operation of phase contrast microscope and fluorescence microscope; Microphotography , the technique of photographic enlargement;Applications of polymerase chain reaction ; Construction, transformation and detection of plasmid in E.coli ; Infectivity of virus and its molecular detection ; The technique of protoplast fusion in filamentary fungi ; Induction and purification of β-galactosidase from E.coli and assay the enzyme activity; Detection of GC content and DNA-DNA hybridization in bacteria ; Production , extraction and detection of poly--hyduoxy butyrate from bacteria.

以实验教学为主，要求研究生学习和掌握一些现代微生物学研究所需的基本技术，内容包括：相差显微镜、荧光显微镜的使用和显微摄影技术，PCR技术的应用，细菌质粒的构建、转化及检测技术，病毒的侵染性及其分子检测技术，丝状真菌原生质体融合技术，大肠杆菌β-半乳糖苷酶的分离及纯化及其动力学测定技术，细菌G+C mol%的测定及细菌DNA-DNA杂交技术和聚羟基丁酸产生菌的发酵、产物提取及检测技术等。

More and more attention has been paid to fungal breeding and gene functional analysis via genetic transformation technology.

利用遗传转化技术对丝状真菌进行菌株改良和基因功能分析，也越来越受到重视。

It is caused by Fusarium which is a large genus of filamentous fungi widely distributed in soil and in association with plants.

这是所造成的镰刀菌，这是一个大属丝状真菌广泛分布於土壤和在协会与植物。

As thermophilic mycelial fungus, Sporotrichum thermophile can decompose cellulose.

嗜热侧孢霉是一种嗜热丝状真菌，具有分解纤维素的特性。

The major contents contained genomics of medical and industrial important microbes; molecular technology used in uncultivable microbes; virus molecular genetics; Streptomyces molecular genetics; filament fungal molecular genetics; yeast molecular genetics and microbial breeding.

本课程主要讲解医学微生物及工业微生物代表菌株的基因组学；难培养微生物的分子生物学研究技术；病毒分子遗传学；链霉菌分子遗传学；丝状真菌分子遗传学；酵母分子遗传学及微生物育种等内容。

There is a strong correlation between intracellular trehalose accumulation and prolonged storage time of conidia from filamentous fungi.

胞内海藻糖积累与丝状真菌孢子的储藏期延长密切相关。

DNA sequence which was partial homogeneous with Copper-transporting P-type ATPase gene was amplified from filaceous fungi Penicillium janthinillum GXCR by designed degenerate primers based on the reported amino acid sequence.

根据已报道的与铜转运P型ATP酶同源的氨基酸序列，设计简并引物，在丝状真菌Penicillium janthinillum GXCR中扩增得到与铜转运P型ATP酶基因同源的部分序列。

Compared to the wild type, the mutants' resistant capacity to Cu~(2+) declined and indicates that the Copper-transporting P-type ATPase gene plays an important role in resistance to heavy metal ion such as Cu~(2+) in filaceous fungi Penicillium janthinillum GXCR

对突变体进行抗铜水平检测发现抗Cu~(2+)能力与野生型对照有下降，表明铜转运P型ATP酶基因在丝状真菌微紫青霉菌Penicillium janthinillum GXCR的抗铜能力上起一定作用。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失