丝状伪足

Result : 1. E xperimental study:(1)After 48 hours culture,the morphology of K562 cells that FFJZ's concentration is 1mg/ml and less than 1mg/ml became fusiform and claviform.The K562 cells that FFJZ's concentration is 2mg/ml has no conspicuous different.The K562 cells that FFJZ's concentration is 4mg/ml and 6mg/ml took on shapes of crenation.

结果：1、体外实验：（1）K562细胞经FFJZ作用48小时后，浓度在 1mg/ml及以下组出现细胞形态呈梭状、棒状，伴有胞浆丝状突起形成伪足，2mg/mlFFJZ组细胞与对照组无明显差别，4mg/ml及6mg/mlFFJZ组，细胞出现皱缩、破碎； 8mg/mlFFJZ及10mg/mlFFJZ组细胞经培养24小时后，即出现细胞明显变小，且均匀分散，以后时间里均呈现此种状况。

MyosinⅩ, a vertebrate-specific member of myosin super-family, can be detected in most tissues and cells, such as kidney, liver, brain, and so on. Full-length MyosinⅩcan stimulate the formation or stabilization of filopodia. As an actin-based motor protein, MyosinⅩplays a key role connecting cell membrane proteins and cytoskeleton.

肌球蛋白Ⅹ是脊椎动物所特有的细胞骨架蛋白，在脑、肝、肾等大多数组织中都有表达，它的显著特点是定位于细胞丝足的顶端和片状伪足的边缘，MyosinⅩ作为一个动力蛋白分子，起到连接细胞膜蛋白与细胞内骨架蛋白的桥梁作用，因此推测它可能在SVZa成神经细胞的迁移过程中起到重要作用。

Gangliosides antagonized the apoptosis induced by corticosteron, and the underlying mechanism is related to activiation of neuronal calcium signal cascade, promoting neurotransmitter release. Gangliosides also have an ability to promote the neuronal filopodia formation and may have a antidepressive-like neuroprotecive effect. 2. Theory study: We explained the clinical symptoms showed in the syndromes of deficiency of kidney-Yang and deficiency of kidney-Yin with modern scientific technical terms. Moreover, we supposed the location of kidney of traditional Chinese medicine may be in the hippocampus but not in the hypothalamus, which is also the centre of learning and memory.

鹿茸有效成分能够拮抗皮质酮诱导的神经细胞凋亡，其机制与影响钙信号途径、促进神经递质释放有关，鹿茸有效成分还能促进神经细胞丝状伪足生长，并且可能具有抗抑郁药样的神经保护作用。2、理论研究：用现代科学语言对肾阳虚证和肾阴虚证临床症状的病理生理机制进行合理的解释，提出中医肾脑相关理论实质假说，推测补肾中药的调控点应当在下丘脑—垂体—肾上腺轴的高级中枢海马，而非下丘脑。

Although we have not a tool to directly modulate the cellular Cdc42/Rac activity at present, nevertheless, from the abolish of BDNF-triggered attraction by either DN-Cdc42 and CA-Cdc42, we propose that an intracellular Cdc42 activity gradient may also be sufficient to determine the turning direction, say, filopodia extend preferentially at the higher Cdc42 activity side, and the growth cone turn towards this direction in the hence.

虽然我们目前还没有一个直接操纵细胞内 Cdc42或其下游分子活性的方法，但从 DN-Cdc42和 CA-Cdc42均阻断到 BDNF 和 ryanodine 的吸引作用来看，我们推测， Cdc42的活性梯度很可能也能够决定生长锥转动方向，即丝状伪足在 Cdc42活性高的一侧优先延伸，生长锥朝向该侧转动。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。