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丝核菌

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After the antagonistic bacterica were cultured for two days, their culture exerted obvious inhibition on pathogenic fungi of Slash pine damping-off such as Rhizoctonia solani and Altemaria olternaia.

在培养2d后,其培养物对丝核菌和链格孢菌均表现出明显抑制效果,但对镰刀菌几乎没有抑制作用。

S024 -strain, which was screened from soil, inhibits Rhizotonia cerealis intensely which can cause Wheat sharp eyespot.

S024菌株是从土壤中分离出来的对引起小麦纹枯病的禾谷丝核菌具有较强抑制力的生防放线菌菌株。

Strain, which was screened from soil by Plant Pathology Lab. ofHenan agricultural university, inhibits Rhizotonia cerealis intensely which can cause Wheat sharp eyespot. Based on previous research, it was found that 024 strain showed its inhibitory effect by producing antibiotic.

024菌株是河南农业大学植物病理研究室从土壤中分离出来的对引起小麦纹枯病的禾谷丝核菌有着强抑制力的放线菌菌株。

As the results,the isolates from Zhejian and Shaanxi were identified as Rhizoctonia solani.

鉴定结果表明,杭州、西安、宝鸡、杨凌等地引起草地早熟禾和高羊茅丝核菌综合症的病原菌为立枯丝核菌

Anastomosis group identification and 5.8S rDNA-internal transcribed spacer sequence analysis showed that the isolates belonged to multinucleate Rhizoctonia AG1-IA, AG1-IB, AG4-HG-I, AG-5 and WAG-Z and binucleate Rhizoctonia AG-A and AG-Ba.

融合群测定及5.8S rDNA-ITS区序列分析结果表明,这些菌株分别属于多核丝核菌的AG1-IA、AG1-IB、AG4-HG-I、AG-5、WAG-Z融合群及双核丝核菌的AG-A、AG-Ba融合群。

The antifungal activities of these materials were investigated and build foundation to develop low poison plant antifungal products. In experiment, polyphenols were extracted by reflux ectraction method which applyed petroleum ether to degrease and methyl alcohol solvent. Phenolic hydroxyl were examined and inspected by colour-developing reaction and the content of total polyphenols was determined by UV-spectrophotometry. Screening test were performed to the antifungal activities of polyphenol extraction by the tube dilution method to the selected ten pathogenic fungus such as Dothiorella gregaria, Rhizoctonia solani, Phyllosticta pirina, and the minimal inhibitory concentration were measured. The column chromatography and thin layer chromatography were applyed to separate and analyse cortex extracts of Dracaena cochinchinensi Lour.

实验部分采用以石油醚脱脂,甲醇为溶剂的回流提取法来提取植物多酚,用显色反应检识酚羟基;采用紫外分光光度法对多酚粗体物进行总多酚含量的测定;选取了聚生小穴壳菌、立枯丝核菌、梨叶点霉等10株常见农林业致病真菌,采用试管液基稀释法对多酚粗提取物进行了抗真菌活性筛选试验,并测定其最低抑菌浓度;采用柱层析法和薄层层析法对其中抑菌效果较好的龙血竭进行分离分析,根据其中3种化合物的光谱数据UV、IR、~(13C-NMR、1H-NMR和MS鉴定其结构。

In this paper, 1554 strains of bacteria were isolated from the soybean rhizophere in the different continuous systems. Those biocontrol bacteria were screened in vitro and in vivo. 6 strains of them had the better control efficacy. They exhibited the different antagonism to the pathogens of soybean root in vitro. The strains 18BR2-2 and 18BR9-1 showed a wide range of antagonism against Fusarium oxysporum, F. avenaoeum, F. solani and Rhizoctonia solani in vitro. The 6 strains exhibited different control efficacy to soybean root rot in greenhouse and in field. In greenhouse, they could decrease the degree of soybean disease significantly, and the control efficacy of 18BR2-2 was the best and can reach 45.90%. In theory, soybean treated by 18BR2-2 may postpone the days of outbreaking of disease for 6 days. The strain 18BR2-2 also promoted the growth of soybean seedling and, raising the soybean height for 11.24%, increasing dry weight for 40.91% and fresh weight for 35.27%.

本研究从长期连作田种植的大豆及其根际土壤中分离细菌1554株,采用平板测试、温室盆栽及田间试验等方法,筛选了对大豆根腐病菌(主要是尖孢镰孢菌Fusarium oxysporum)有很好作用效果的生防细菌6株,在离体的条件下均表现出不同程度的拮抗作用,其中生防细菌18BR2-2和18BR9-1表现出很广的杀菌谱,对大豆根腐病菌尖孢镰孢菌(F.oxysporum)、燕麦镰孢菌(F.avenaoeum)、茄腐镰孢菌和立枯丝核菌都有很好的离体拮抗作用,并且这6株生防细菌在盆栽和田间的情况下均表现出对大豆根腐病一定的防治效果。

The inhibitory rate of metabolites produced by TD103-UV-4 against RS was more than 80%, and the antibiotic production capacity increased by 47.1%, meanwhile, its metabolites had a strong inhibition against other phytopathogenic fungi, such as Botrytis cinerea.

其中菌株TD103-UV-4发酵液对立枯丝核菌的抑制率在80%以上,并且对灰葡萄孢菌等植物病原菌有较强的抑制作用,产抗生素能力比出发菌株提高了47.1%。

Of 549 isolates, 20 isolates (about 3.6% of the total isolates) showed significant activity against P. ultimum and 15 isolates (about 2.8% of the total isolates) against R.

结果表明,对终极腐霉、立枯丝核菌的半抑制稀释倍数(ID50)在10以上的活性菌株分别为20株(占总菌株的3.6%)和15株(占总菌株的2.8%)。

On soil fungi, Fusarium root shell mold, Rhizoctonia, Pythium, Miao-rot fungus, Corticiumpathogens have a significant effect on the damping-off,diseases, damping-off, Fusarium wilt, Verticillium, Sclerotinia, Colletotrichum, disease, dry rot, scab, Sclerotinia rot, seedling blight, stem blight, leaf blight, steep root, even the special effects for the continuous cropping obstacles.

对土壤真菌、镰刀菌、根壳菌、丝核菌、腐霉菌、苗腐菌、伏革菌等病原菌都有显著的防治效对立枯病、烂秧病、猝倒病、枯萎病、黄萎病、菌核病、炭疽病、疫病、干腐病、黑星病、菌核软腐病、苗枯病、茎枯病、叶枯病、沤根、连作重茬障碍有特效。

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