丛状层

In the brain of adult rat, the positive immunohistochemical product of lSL-l (ISL-l-positive) was mainly located in the neuronal nucleus and found in discrete regions except to brain cortex, such as the Purkinje cell layer and the granular cell layer of cerebellum, the granular cell layer and the pyramidal cell layer of hippocampus, the mitral cell layer, the internal and external plexiform layer, the granular cell layer and the granular cell layer of olfactory bulb and so on, and several nuclei of the hypothalamus, midbrain and pons, such as claustrum, anterior olfactory nucleus, accumbens nucleus, caudate-ptamen, pallidum, substantia nigra, striatum, islands of Callaje, mammillary nucleus, anterior pretactal nucleus, habenular nucleus, amygdaloid nucleus, cuneate nucleus, rubral nucleus, gigantocellular reticular nucleus and so on.

在正常成年大鼠脑中，同源框基因islet-1表达产物（ISL-1）免疫组织化学阳性物质广泛分布于除大脑皮层外的神经细胞的细胞核内，ISL-1阳性神经元密集分布于小脑Purkinje细胞层和颗粒细胞层、海马的颗粒细胞层和锥体细胞层、嗅球的内丛层、外丛层、颗粒细胞层及僧帽细胞层等，另外在丘脑、中脑和桥脑的一些重要神经元核团均有分布，如，屏状核、前嗅核、伏核、尾壳核、苍白球、黑质、纹状体、Calleja岛、乳头体核、前顶盖前核、缰核、杏仁核、楔束核、红核网状巨细胞核等。

The cilia of retinal pig merit epithelium disappeared completely by TEM, the granola of retinal pigment epithelium decreased, the rough endoplasmic retinal, the mitochondrial crista breaks, the outer nuclear layer arrangemen disorderin, disc broad, the vacuole timer and outer plexi-form layer shaped, the ganglion cells and membranous of eye cell outer side got think, intermembranous space gotorgancelle disappeared mostly, the crista of cytoplasm ganglion cells layer swelled.

透射电说可见：视网膜色素上皮细胞表面纤毛完全消失，视网膜色素上皮细胞内颗粒减少，粗而内质网、线粒体嵴断裂，外颗粒层细胞排列紊乱，视细胞外段盘膜粗大，盘膜间隙增宽，内外丛状层空泡形成，神经节细胞、细胞器大部分消失，神经节细胞层可见细胞质有嵴性肿胀。

The outer neuroblastic layer before it divided to inner and outer nuclear layer and outer complexiform layer and gonglion cell layer showed a temporal PCNA positive which was related to proliferation. The three cell layers had another PCNA positive when finished time changed from outer to inner after their morphologic development basically completed( outer nuclear layer:11～15 days,inner nuclear layer: 8～16 days, gonglion cell layer:16～21 days).

PCNA显示在外成神经细胞层分化为内、外核层和外丛状层之前，和节细胞各有一次和增殖相关的阳性表达。3个细胞层在形态学的分化基本完成后，又出现了一次结束时间是由外向内变化的阳性过程（外核层：第11天至15天：内核层：第8天至16天，神经节细胞层： 16天到21天，这一次阳性表达过程可见到细胞形态向成年形态的转化。

RESULTS: VIP-immunoreactive nerve fibers were expressed from apical foramina to the central pulp as bunchy line,and became fan-shaped at the cervical line,more branches in the coronal pulp.Some branches surrounded blood vessels,some branches terminated in the pulp matrix,the others passed Raschkow nerve plexus and run forward the odontoblastic layer and the predentine,but not went into dentine.

结果：VIP阳性神经纤维自根尖孔呈束状进入牙髓，至颈部扇形分开，在冠髓大量分支，部分围绕在血管周围，部分终止于牙髓基质，部分参与形成成牙本质细胞层下Raschkow神经丛，然后发出分支伸入成牙本质细胞层和前期牙本质，但不进入成熟牙本质。

Based on the results, it has found that six distinct layers can be identified from lateral to medial in the olfactory bulbs, and the adults have more cells than the subadults. Ventricles of the adults take up larger vacuity, have richer choroids plexus, and primordial pallium and primordial hippocampus of them are more developed than the subadults, in which the primordial piriform is well-developed. Ventral to the primordial hippocampus, there is a septal area which can not be divided into medial and lateral parts in both adults and subadults, nor there is lateral limiting sulcus to separate the primordial piriform and the corpus striatum, but only in the subadults, a medial limiting sulcus can be clearly identified to separate the primordial hippocampus and the septal area. Besides, the corpus striatum of Onychodactylus fischeri is paleostriatum. The cell group that located at two sides of the third ventricle is amygdale, and shape and size of neurons within the telencephalon are poorly differentiation.

结果表明：爪鲵嗅球内细胞从外到内大致可分为6层结构，亚成体的细胞数量少于成体；成体爪鲵的侧脑室所占空间较大，脉络丛更为丰富，原始大脑皮层和原始海马都比亚成体发达，后者大脑内原始梨状区较为发达；隔区位于原始海马的下方，成体和亚成体都没有外侧隔核和内侧隔核之分，也没有外侧界沟作为原始梨状区和纹状体的分界，而在亚成体的侧脑室壁有内侧界沟区分原始海马和隔区，成体则没有；此外，爪鲵的纹状体属于古纹状体，杏仁核是位于第三脑室两侧的两个细胞团，端脑内细胞形态和大小分化较为单一。

RESULTS: A moderate expression of GAP43 was observed in the inner plexiform layer of the retina in normal rabbit eyes.

结果：正常兔眼视网膜内丛状层中存在GAP43的表达。

RESULTS: VIP widespread expression existed on photoreceptor-outer, inner nuclear layer, inner plexiform layer of retinal and choroids; weak expression existed on ganglion cell layer and nerve fiber layer.

结果： VIP的表达主要位于视网膜光感受器外节、内核层、内丛状层和脉络膜，在神经节细胞层和神经纤维层有较弱表达。

In Wistar rats, latent period of a-wave in scotopic rod response after 1-week transplantation was shorten (P.05); and latent period of a-wave in scotopic rod response after 2-week transplantation was prolonged (P.05); while latent period of b-wave in scotopic maximum response after 1-week transplantation was shorten (P.05). In RCS rats, amplitude of a-wave in scotopic rod response and photopic response after 4-week transplantation was increased (PO.05); while latent period of a-wave in scotopic rod response after 4-week transplantation was shorten (PO.05).Conclusion 1. At the age of 3 months, photoreceptors of the RCS rat degenerated completely, which leaded to the reduction of amplitudes of a-wave and b-wave.

Wstar大鼠术后1周的暗适应视杆反应，a波潜伏期缩短o叼。05）；术后2周的暗适应视杆反应，a波潜伏期延长o功刀5）：术后1周的暗适应最大反应，b波潜伏期缩短o功刀5卜＊CS大鼠术后4周的暗适应视杆反应和明适应反应，a波振幅增大o功刀匀；术后4周的暗适应视杆反应，a波潜伏期缩短o＜0卜结论1、RCS大鼠在3月龄时感光细胞变性死亡，导致FERG的a、b波振幅明显降低；视网膜下腔仍存在感光细胞外节盘膜碎屑，外丛状层缺乏感光细胞的突触末端，同时Muler细胞出现反应性胶质纤维增生。

RESULTS: In the retinal region without light, the structure of every layer was clear, cells in neuroepithelial layer arrayed in rule, some bubble presented in external granular layer and internal granular layer, RPE cells were compact, and the color of pigment article was coincident. In the region with direct blue light and that with 30J/cm2+Acrysof one-piece/ pMMlA, cells on photoreceptor and external granular layer were lost partially, bubble increased, RPE cells were with different sizes, and cell edema, cell lost and pigment article cluster could be seen.. In region with 30J/cm2+Natural, a little disorganization could be seen comparing to that without light, but more normal than those with Acrysof and direct eradia tion.

结果：未照射部位：视网膜各层次结构清晰，神经上皮层内各细胞排列规则，整齐，RPE细胞排列紧密均匀，色素颜色一致；单独光照和透过Acrysof一片式I0L：光感受器层部分细胞丢失，外核层可见固缩核，浓密，内颗粒层细胞大量丢失，内丛状层内空泡明显增多，RPE表面，细胞大小不均，可见肿胀、缺失，细胞内色素浓密、聚集成块；透过Nat-ural一片：与未照射处相比，视网膜各层次结构仍有部分破坏，但程度明显减轻。

The data were analyzed by analysis of variance between the control group and experimental group and by analysis of snk-q among each dosage in the experimental group.results: vip widespread expression existed on photoreceptor-outer, inner nuclear layer, inner plexiform layer of retinal and choroids; weak expression existed on ganglion cell layer and nerve fiber layer.

结果： vip的表达主要位于视网膜光感受器外节、内核层、内丛状层和脉络膜，在神经节细胞层和神经纤维层有较弱表达。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失