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丙酮血

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Objective:Analysis of screening results of three neonatal diseases to further clarify the incidence of phenyl ketonuria,congenital hypothyroidism and glucose-6-phosphate dehydrogenase(G6PD) deficiency in Hainan Province.

目的:对海南省新生儿疾病筛查情况进行分析,了解海南省新生儿苯丙酮尿症、先天性甲状腺功能低下症、葡萄糖6-磷酸脱氢酶缺乏症(G-6-PDD)的发病率。方法:对出生72h后的7412名新生儿采集足跟血制作血滤纸干标本,PKU筛查采用荧光定量法检测滤纸干血斑中Phe含量;CH筛查采用时间分辨荧光免疫法检测滤干血斑中TSH含量;G6PD缺乏的筛查采用荧光斑点定性试验测定G6PD活性。

And the effect of the percentage of hydrochloric acid to acetone,the volume ratio of hydrochloric acid acetone to hematolysis solution were researched.Results:The supersonic wave hemolysis was Established.

结果:确立超声波法进行红细胞溶血,36.5%浓盐酸与丙酮的体积为1%的血红素抽提液,与溶血液的体积比为5∶1抽提10分钟,最终可从新鲜猪血中得到红素6.0 g/L,纯度达99.8%。

Methods:The fresh porcine blood added sodium citrate was used for extracting of heme. The methods of hematolysis by water,ethanol and supersonic wave were compared.

新鲜抗凝猪血为原料,比较了水溶胀法、乙醇法以及超声波法对红细胞溶血效果,以盐酸丙酮配比,盐酸丙酮与溶血液体积比对猪血中血红素提取的影响。

Central venous pressure, mean arterial pressure, the ratio of lactic acid to pyruvic acid in venous blood, systemic inflammative response syndrome, volume of blood and fluid administrated intra- and post- operatively, complications and the therapeutic outcomes were determined in each group.

分别测定各组病人术后中心静脉压、平均动脉压、静脉血乳酸与丙酮酸比值、术中和术后输血、输液量、全身性炎症反应综合症发生率、并发症发生率和治疗结果。

A clinical diagnosis of MELAS was made.

血乳酸和丙酮酸水平升高,临床诊断MELAS。

Properties : White crystallization or powder , proportion 1.735 , melting point is 287 ℃, apt to dissolve in water , ethanol and propanone , aqueous solution is neutral , turn into the blood red sulphur cyaniding iron with molysite , reactionless with inferior molysite, salt function turns into dark blue sulphur cobalt cyanide, turn into white sulphur silver precipitating or black sulphur copper cyanide cyanide to precipitate with the silver salt or copper salt function with cobalt.

白色结晶或粉末,比重 1.735 ,熔点 28 7 ℃,易溶于水、乙醇和丙酮,水溶液呈中性,与铁盐生成血红色的硫氰化铁,与亚铁盐则无反应,与钴盐作用生成深蓝色的硫氰化钴,与银盐或铜盐作用生成白色的硫氰化银沉淀或黑色的硫氰化铜沉淀。

Results: Comparing with movement fatigue group, blood glucose contents were significantly increased but oxypropionic acid and urea nitrogen contents were significantly increased; testosterone and T/C were significantly advanced, myocardium SOD activity was significantly increased, while MDA was significantly decreased in cluytyl alcohol preparation group.

结果:和运动疲劳组相比,补充二十八烷醇制剂组大鼠血糖显著升高,血乳酸、血尿素氮显著降低;T、T/C比值显著增高,心肌SOD活性显著增强,MDA含量显著降低,且效果优于丙酮酸-肌酸组。

BH loading tast and urine pterin analysis can be used as differental diagnosis methods for classical PKU and BH deficiency.

血苯丙氨酸浓度在口服BH后无明显变化,尿喋呤谱分析基本正常的患儿诊断为经典型苯丙酮尿症

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