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The homology is extensive throughout the protein and all amino acid residues of Pdc that have been shown to be involved in ThDP binding, both of the thiazole and phosphate moieties, are precisely conserved in Thi3p.

整个蛋白具有广泛的同源性,并且丙酮酸脱羧酶的氨基酸残基,已被证明参与了ThDP结合,双方的噻唑和磷酸基,都精确的保存在Thi3p中。

Primary extract the active elements from the root,stem and foliage of Artemisia annua L with petroleum ether (30~60℃), petroleum ether (60~90℃), ethanol, acetone and water were carried out in laboratory, then further separation of active elements was detected by column chromatography, and then the separated elements′acaricidal activitie was measured against Tetranychus cinnabarinus.

采用石油醚(30~60℃)、石油醚(60~90℃)、乙醇、丙酮和水等5种溶剂对采自6月份的黄花蒿的根、茎、叶分别进行活性成分的初步提取,然后用柱层析进行分离检查,并进一步用分离得到的组份对朱砂叶螨进行生物测定。

In the design of the ink formulations, to fully take into account the ability to eliminate static electricity on innovativeink, you can formula 2% of acetonic, at the same time adding 0.1%~ 0.5% AE-2 Super concentrated anlistatig or 0.5%~ 2% 3500A anlistatig settlement.

在安排油不朱的配方时,也给充足不天思考到油不朱打消静电的本领,可以在配方洋增入2%~8%的异丙酮,同时增入0.1%~0.5%的AE-2超不浓缩抗静电剂或0.5%~2%的3500A抗静电剂举行处置。

The producing of tetrapod-like CuCl was found to be the result of co-effect of acetylacetone and ethylene glycol.

通过对溶剂所起的作用的研究,发现四足形CuCl晶体的形成是乙二醇和乙酰丙酮协同作用的结果。

These new ionic liquid-supported acetylacetone catalysts were used for the oxidation of the intermediates of Vitamin E (2,3,5-trimethylphenol and 3,5,5-trimethylcyclohex-3-en-l-one). It not only checked the rationality of the design, but also established the base for the green oxidation of corresponding reactions.

论文进一步将此类离子液体支载乙酰丙酮金属催化剂应用于维生素E中间体三甲基苯酚和β—异佛尔酮的氧化过程,一方面检验了催化剂设计过程及结果的可靠性,另一方面为相关氧化反应的绿色化改造提供相关的基础。

In order to explore the extraction method of proteome and technique of 2-DE, we compared the extraction method between the TCA-ac-tone and the Tris-HCl, and improved the key steps of 2-DE. The Tris-HCl extraction was the most appropriate for Antiaris toxicaria seed proteome analysis because of the highest resolution and more informative 2-DE map. Those were the solid basis for the later research of studying on the discrepancy expression proteomics of Antiaris toxicaria Seed.

在探索适合箭毒木种子蛋白质组学研究双向电泳方法中,比较了三氯乙酸-丙酮沉淀法、和Tris-HCl法,以及对双向电泳过程中的关键步骤的改良,认为Tris-HCl法为最适方法,所得图谱背景清晰,蛋白质信息量最大,为箭毒木属植物的差异蛋白质组学的后续研究打下了坚实的基础。

ResultsMost coixendide could be extracted by adding 3.5 times of acetone into the dry coix powder for 3 times and extraction for 8hs each time.

结果用3.5倍的丙酮浸提3次,8 h/次,能萃取出薏苡仁中大部分的薏苡仁酯;壁材明胶水溶液的浓度为20%,芯材薏苡仁酯与壁材明胶的质量之比4∶10。

In the second part of the thesis, we discussed a L-proline-catalyzed direct Aldol reaction between L-amino acid-derived N,N-dibenzyl -amino aldehydes 15 and acetone, cyclopentanone or hydroxyacetone, which afforded (-amino--hydroxy- or (-amino-,(-dihydroxy-ketones in good to excellent yields (up to 94%) and diastereoseletivities (up to 94.9%).

发现许多情况下,反应的产率和de值超过90%。接着,在碱性条件下,我们将L-亮氨酸衍生的N,N-二苄基--氨基醛15g与羟基丙酮反应得到的主要产物(,-二羟基-γ-氨基酮171d与次溴酸钠反应,得到了具有抗肿瘤活性的天然产物PM-94128的氨基酸片断184。

The extraction and stability of the pigments in Tussilago farfara L. were studied. The experimental results showed that the pigments could be solved in acetone. The stability of the pigments is good when pH is within 3~9; the oxidizing agents, reducing agents and common food additives have little influence on its stability; it is react too much with Fe(superscript 3+) ion, and Na(superscript +) ion showed a certain increment effect on the color; the heat performance of the pigments is good when under 60℃.

研究了款冬花色素的热稳定性、氧化还原稳定性和pH值稳定性,并对常用的几种食品添加剂对款冬花色素的稳定性进行了探讨,结果表明:款冬花色素易溶于丙酮,属于脂溶性色素;在pH=3~9时,款冬花色素的稳定性较好;氧化还原剂及食品添加剂对其稳定性影响不大,Fe(上标 3+)金属离子对其影响显著,Na有增色作用;水浴温度在60℃以下色素的热稳定性较好。

The colour of most detected bands became light in the five media with the order of mannosan, mannose, xylan, glucose and pectin.(4)The pectinase in the supernatant was purified by centrifuge , acetone precipitation and sephadex G-75 cloumngel filtration. The specific activity was increased 9.8-fold with an activity recovery of 32.98%.The molecular weight of pectinase was 42.2KD estimated by SDS-PAGE.

确立了以葡萄糖培养基6h的发酵液为提取果胶酶的最初取样点,发酵液经离心,丙酮沉淀,Sephadex G-75葡聚糖凝胶柱层析后获得了凝胶电泳均一的样品,比活力较原酶液提高了9.8倍,回收率达到了32.98%,用SDS-PAGE凝胶电泳测的纯化后的果胶酶分子量为42.2KD。

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