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To explore the relationship between the change of M2-PK level in plasm with the occurrence of gastric cancer and the diagnostic value for gastric cancer.

目的探讨血浆M2-丙酮酸激酶(M2-PK)含量变化与胃癌发生的关系及M2-PK对胃癌的诊断价值。

According to the result showed at 280nm and at 490nm,in the comparison of whether protein absorption top and sugar quantity top overlapped,glycoproteins would be detected preparatorily,and as a result,tubes in two distinct areas had glycoproteins by this method. Proteins were precipitated with trichloroaceticacid and with cold acetone,and glycoprotein was determined from SDS-gel.

再从各收集管的收集液中,用三氯乙酸沉淀蛋白法、冷丙酮沉淀蛋白法相结合浓缩、制备蛋白样品,进行SDS—PAGE,对SDS胶进行PAS糖链染色鉴定糖蛋白,并从茶树叶分级蛋白中准确地鉴定出两个区域的收集管中含有多种糖蛋白。

With the in-situ single-stage self-metallization protocol,surface-silvered polyimide film has been prepared using pyromellitic dianhydride/ 4,4\'-oxydianiline-based polyimide as the matrix and (1,1,1-trifluoro-2,4-pentadionato)silveras the silver precursor.

在原位一步自金属化法中,以均苯四甲酸酐/4,4\'-二胺基二苯醚基聚酰亚胺为基体,以三氟乙酰丙酮银作为银的母体制备出了表面金属化的聚酰亚胺银复合薄膜。

Methods: We use the content of pyroracemic acid to show the activity of alliinase in onion, investigated different temperature, pH and metal ions, and other conditions impact the activity on the alliinase.

采用丙酮酸法测定洋葱中蒜氨酸酶的活力,考察不同温度、pH值和金属离子等条件对蒜氨酸酶活力的影响。

When BMSCs proliferated to enough cell number (7×106). 500 000 cells were centrifuged to a pellet and cultured in chondrogenic medium supplemented with DMEM high-glucose, insulin, transferring, selectin, pyroracemic acid, 2-pyrrolidine carboxylic acid, BSA, dexamethasone and ascorbic acid for 6 weeks.

当培养的骨髓基质干细胞达到7×106个细胞时,将每500 000个细胞离心制成高密度细胞微球,在含有DMEM高糖、胰岛素、转铁蛋白、选择素、丙酮酸、脯氨酸、 BSA、地塞米松、抗坏血酸的促软骨细胞分化培养液培养6周。

When BMSCs proliferated to enough cell number (7×10^6). 500 000 cells were centrifuged to a pellet and cultured in chondrogenie medium supplemented with DMEM high-glucose, insulin, transferring, selectin, pyroracemic acid, 2-pyrrolidine carboxylic acid, BSA, dexamethasone and ascorbic acid for 6 weeks.

当培养的骨髓基质干细胞达到7×10^6个细胞时,将每500000个细胞离心制成高密度细胞微球,在含有DMEM高糖、胰岛素、转铁蛋白、选择素、丙酮酸、脯氨酸、BSA、地塞米松、抗坏血酸的促软骨细胞分化培养液培养6周。

Several methods of plant tissue protein extraction (TCA-acetone precipitation method, phenol method, urea method, Zhou Han-tao described method, and phenol method improved by the authors) were applied to extract total protein from Rhizophora stylosa root, and then after SDS-PAGE, a subsequent 2-DE analysis was carried out with the optimized conditions for 2-DE .

分别采用TCA-丙酮沉淀法、酚法、尿素法、周涵韬法和笔者建立的酚改良法,提取红海榄根部总蛋白质,并进行了SDS-PAGE分析和双向电泳体系的优化。

Acetone and SDS have been added to CH2CL2 solution containing PLA. After sonification of 20 minutes, this polymer solution has been dropwisely added to aqueous solution containing 8-14% Pluronic F-68 surfactant and stirring with homogenizer (25000 rpm). With rota-evaporator to remove solvent, polyester nanoparticles possessed average size between 61 and 64 nm. Other combinations of surfactants, such as : SDS and PVA, PAA and SDS, used in preparation of nanoparticles of polyester, resulted in much larger particles compared to above.

丙酮和十二烷基硫酸钠加入溶於二氯甲烷的聚乳酸中,并经超音波振荡20分钟,所产生的高分子微粒溶液再滴入以均质机搅拌(25000 rpm)含有(8-14%) Pluronic F-68界面活性剂溶液中,经减压浓缩除去溶剂后,奈米聚酯微粒平均粒径大小为61-64 nm,其他界面活性剂组合,包括十二烷基硫酸钠与PVA、PAA与SDS,其产生聚酯平均粒径皆大於此。

Krebs cycle (citric acid cycle; TCA cycle; tricarboxylic acid cycle) A complex cycle of reactions in which pyruvate, produced by glycolysis, is oxidized to carbon dioxide and water, with the production of large amounts of energy.

卡尔文循环(柠檬酸循环, TCL 循环,三羧酸循环):糖酵解过程中产生的丙酮酸,被氧化为二氧化碳和水并产生大量能量的复杂反应过程。

Five species of solvents, namely, acetone, ethyl acetate, ether, trichloromethane and petroleum ether were selected to obtain extracts from Portulaca oleracea L. with microwave assistance and the biological activities of each extract was tested against Plutella xylostella L.

在微波加热条件下,以丙酮、乙酸乙酯、乙醚、三氯甲烷和石油醚为溶剂对马齿苋干粉进行提取,试验了各溶剂提取物对小菜蛾幼虫的触杀、胃毒及拒食活性,确定了石油醚提取物触杀作用的LD50。

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