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Results Same conservative sites and key catalytic sites existed among SjLDH and LDHs from other species. Similarity of SjLDH compared to CsLDH, TvLDH and HsLDH was 75%, 17%, 58%-60% respectively. Phylogenetic analysis demonstrated that the evolution relation between SjLDH and DmLDH was closer than the relation between SjLDH and CeLDH, the relationship between SjLDH and HsLDH-B,-C was closer than HsLDH-A. Three trans-membrane regions were found, the region of 98aa-106aa in three hydrophilia regions located outside of membrane was inferred as the major antigen epitope. This antigen epitope had significant difference with LDHs from protozoon ( Pf ., Tg ., Tv .) and had 1-3 amino acid residue difference with MmLDH, HsLDH-A,-B,-C, and was the same with CsLDH. One of the key catalytic residues and substrate binding loop were located in this region. Tertiary structure demonstrated that 98aa-106aa was on the surface of the protein and formed a substrate binding loop, other two key catalytic sites were at the position near the loop.

结果 SjLDH与其他物种的同源序列含相同的保守位点及催化活性位点,与华支睾吸虫LDH同源性最高为75%,与阴道毛滴虫LDH同源性最低为17%,与人LDH(HsLDH-A,-B,-C)的同源性为58%~60%; SjLDH与果蝇的进化距离较秀丽隐杆线虫为近,3种人LDH中与HsLDH-B、-C的进化距离较HsLDH-A为近;该蛋白具有3个跨膜区域,3个高亲水性区域,主要的线性表位98aa~106aa位于膜外,与原虫LDH相同区域差异显著,而与其他LDH有1~3个氨基酸的差异,关键催化位点之一及底物丙酮酸结合区域位于该区域,蛋白质同源模建分析表明该区域位于蛋白表面形成环状结构,3个关键催化位点位于该区域或在其附近。

Our use Co2 as a catalyst in this work, exploring new way to produce nitrobenzoic acid from nitrotoluene under the prue oxygen in basic medium.

本文探讨了以乙酰丙酮钴为催化剂,分子氧为氧源,在NaOH-CH_3OH的反应环境下,氧化硝基甲苯制备硝基苯甲酸的新方法。

The pyroligneous acid also contains acetic acid which can be converted into calcium acetate, from which acetone can be obtained as in.

焦木的酸并且包含可能被转换成钙醋酸盐,从丙酮作为可能被获得的乙酸。

Study showed that a mixture of Pyroxylin and alloy powder and acetone,which was deposited on the substrate, can help to form a high performancecoating.

工艺实验结果表明,用一定量的硝酸纤维素和 MCrAlY 合金粉末混合后,用丙酮调成糊状,涂覆于基材表面的方法,在后续的激光熔覆中能形成高质量的 McrAlY 抗氧化涂层。

[Objective]To investigate the effects of calcium pyruvate on exercise ability in mice for 3 weeks.

[目的]探讨补充3 周丙酮酸钙对于小鼠力竭运动能力的影响。

In aerobic respiration, therefore, pyruvate is completely oxidized to carbon dioxide and water.

因此,在有氧呼吸中,丙酮酸被完全氧化成二氧化碳和水。

This article studies the synthesis of sodium pyruvate from tartaricc acid.

本文研究了酒石酸合成丙酮酸钠的工艺。

Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery.

简单、直接、自动化测量丙酮酸浓度的手段,在研究和药物研发中受到广泛的应用。

Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders.

人体内丙酮酸的非正常水平与肝脏疾病和代谢疾病有关。

The effect of amino acid on production of pyruvate by T. glabrata CCTCC M202019 was investigated.

在维生素限量添加的条件下,研究了添加氨基酸对丙酮酸发酵的影响。

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