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The said glutamine synthetase is prepared by abrupt change of the 405th amino acid residue of amino end to phenylalanine corynebacterium glutamicum.

该谷氨酰胺合成酶是自氨基端第405位氨基酸残基突变为苯丙氨酸的谷氨酸棒杆菌的谷氨酰胺合成酶。

With the help of RP-HPLC, the hydrolyzes of corn gluten meal were found to be rich in Pro and basic amino acid, such as Phe and Leu, which can be used as food additives to supply basic amino acid and to lower blood pressure.

利用反相高效液相色谱法对玉米黄粉蛋白酶解产物的氨基酸组成进行了测定,发现酶解产物中含有大量的Phe(Phenylalanine,苯丙氨酸),Leu(Leucine,亮氨酸)等必需氨基酸和Pro(Proline,脯氨酸),他们可以添加到食品中来补充必需的氨基酸和降低高血压患者的血压,对综合开发利用这一丰富的农副产品具有重要的意义。

L-Phenylalanine was then obtained by alkylation of N- glycine ethyl ester with benzyl bromide in the presence of an asymmetric phase-transfer catalyst-N-benzyl-cinchonine chloride, followed by hydrolysis of the resulting product.

以甘氨酸乙酯盐酸盐和二苯甲酮为起始原料合成了N-二苯亚甲氨基乙酸乙酯,后者在手性相转移催化剂-N-苄基氯化辛可宁诱导下与烷基化试剂溴化苄发生不对称相转移催化烷基化反应,水解得到L-苯丙氨酸

Thirty-one new chiral phosphorus compounds have been synthesized starting from D-camphor,-alpha-phenylethylamine,-Betti base,-1-(4-nitrophenyl)-2-amino- propane-1,3-diol, L-amino acids (such as serine, phenylalanine, proline etc.) and their application as organocatalysts in asymmetric trimethylsilylcyanation of aldehydes and asymmetric borane reduction of prochiral ketones and ketimines were investigated.

该项目从D-樟脑、-alpha-苯乙胺、-Betti碱、-氯酶胺、L-氨基酸(如丝氨酸、苯丙氨酸、脯氨酸等)出发合成了31个新的手性磷化合物,研究了它们作为有机催化剂在醛的不对称硅氰化反应、潜手性酮的不对称硼烷还原反应以及亚胺的不对称硼烷还原反应中的催化效果。

The chemistry of CAPAs was almostunexplored since it was proposed in a prebiotic reaction of inorganic polyphosphates with amino acids.

自CAPA的结构被提出以来,CAPAs的化学反应几乎没有得到进一步的研究,所以本论文的第二章研究了以甘氨酸-CAPA和丙氨酸-CAPA为代表α-CAPAs分子,通过同位素标记实验~(18O,~(15)N证明了CAPA分子具有两个亲电中心。

In chapter 2,the bi-electrophilicity ofα-CAPAs(Gly-CAPA,Ala-CAPA) was identified by the isotope analysis ~(18O,~(15)N and furtherproved by trappingα-CAPA with nucleophiles such as water,amino acids,phosphateand methanol in alkaline media which yielded phosphorylated products,namely,theNPAAs and N-(mono-substituted)phosphoryl amino acids.

自CAPA的结构被提出以来,CAPAs的化学反应几乎没有得到进一步的研究,所以本论文的第二章研究了以甘氨酸-CAPA和丙氨酸-CAPA为代表α-CAPAs分子,通过同位素标记实验~(18O,~(15)N证明了CAPA分子具有两个亲电中心。

Obstructive jaundice was induced in the mice in the experimental group by common bile duct ligation. The level of the common bile duct diameter, WBC, LYM MID, LYM%, MID% and ALT, AST, TBIL, DBIL, IBIL, ALP and CHOL were measured 12 h and 1, 2, 3, 4, 5, and 7 days after the ligation.

于实验的第12 h,1、2、3、4、5、7 d动态观察胆总管直径及白细胞总数、淋巴细胞总数、中间细胞总数及淋巴细胞总数百分数和中间细胞总数百分数,测血清总胆固醇、丙氨酸氨基转氨酶、天冬氨酸氨基转氨酶、碱性磷酸酶、总胆红素、直接胆红素及间接胆红素值。

Firstly, synthetic racemates of β- amino acids were resolved into their enantiomers by ehiral ligand - exchange flash chromatography with L - hydroxyproline chemically bonded to irregular silica as the stationary phase and aqueous solution containing cupric ions as the mo

首先合成L-羟脯氨酸硅胶键合手性固定相,用于快速色谱中3-羟基-4-甲氧基-β-苯丙氨酸对映体的制备分离,考察了流动相中Cu^2+浓度、样品体积等因素对分离的影响。

Content (and to a very small extent on the amount of Phe and disulfide bonds).

在紫外线附近,蛋白质的光吸收决定于酪氨酸和色氨酸的含量(苯丙氨酸和二硫键有小量影响

The results show that during the development of the oriental tobacco leaves, the activity of acid invertase was decreased; the changes of GS reached its peak in the 20dans in Samsun, but in the 30dans in Basma and Canik ;the activity of PAL was decreased in the 30dans; The content of total nitrogen was decreased with the mature leaves, but nicotine、total carbon and soluble total sugar were increased. Different varieties, carbon and nitrogen metabolism key enzyme activity and the content of metabolites were different.

结果表明,在香料烟叶片发育过程中,酸性转化酶的活性均呈下降趋势;谷氨酰胺合成酶的活性Samsun品种在20d叶龄时最高,而Basma和Canik在30d叶龄时最高;苯丙氨酸解氨酶的活性均表现为30d时最低;随着叶片成熟总氮含量降低,烟碱含量升高,总碳、可溶性总糖随着叶片成熟其含量呈升高趋势;品种不同,碳氮代谢关键酶的活性及代谢产物的含量变化均有很大差异。

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