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The composition of amino in feed matter and feed draff after degradation for sixteen in rumen compared, from which we found the content of vary of amino acids in peanut meal and Soybean meal had increasing trends after sixteen hours cultured in rumen. The increasing trends of leucine, isoleucine, threonine, phenylalanine were greater. The increase trends of lysine, methionine, arginine and histidine were smaller. The content of varies of amino acid in cotton seed meal matter had decreasing trend s after sixteen hours cultured in rumen, but decreasing value was small. The compositions of amino acid in lees and rape seed meal matter were steady after sixteen hours cultured in rumen.

通过把饲料原料中氨基酸的组成和瘤胃降解16h后饲料残渣的氨基酸组成比较发现各种蛋白质饲料瘤胃降解前后氨基酸组成均发生了一定的变化,总的来说,氨基酸浓度在降解后有上升趋势(p<0.05)的有亮氨酸、异亮氨酸、苯丙氨酸、缬氨酸、苏氨酸、丙氨酸和脯氨酸,氨基酸浓度在降解后有下降趋势(p<0.05)的有赖氨酸、精氨酸、谷氨酸和胱氨酸,氨基酸浓度在降解后差异不显著的有组氨酸、天门冬氨酸、甘氨酸,降解后无明显规律的有蛋氨酸、丝氨酸和酪氨酸。

Methods Gelators of N-lauroyl-l-alanine methyl esterand N-lauroyl-l-alanine ethyl esterwere synthesized with l-alanine methyl ester,l-alanine ethyl ester and lauroyl chloride as materials.

方法以l-丙氨酸甲酯盐酸盐、l-丙氨酸乙酯盐酸盐和月桂酰氯为原料,合成月桂酰-丙氨酸甲酯凝胶因子及月桂酰-丙氨酸乙酯凝胶因子,采用IR、1H-NMR、MS对其结构进行表征,并对其熔点、热稳定性、在各种植物油中的最小胶凝质量浓度、相转变温度等基本性质进行了研究。

Development period of the parasitoid pupa from the 5th instar larva fed on MSB was significantly longer. Length of cocoon of MSB treatment was significantly shorter than the control. However, no obvious differences were detected in the development periods of egg to larva, cocoon mass size, wasp emergence rate, sex ratio, longevity and forewing length of wasp between KMD1 and the control. 3. Amino acid content in the haemolymph of SSB after fed with transgenic rice Although there were no changes on the components of amino acids in the haemolymph of SSB after fed with two transgenic rice lines, there were some changes on relative contents of amino acids.

取食KMD1 36h后0天,20种氨基酸中除天门冬酰胺、丝氨酸、苏氨酸和甲硫氨酸外,其它16种氨基酸的含量都高于对照,其中组氨酸、甘氨酸、苯丙氨酸、异亮氨酸、赖氨酸差异达显著水平,谷氨酰胺、脯氨酸差异达极显著水平,与氨基酸总量表现一致的变化趋势;取食KMD1后1天,除天门冬酰胺、谷氨酰胺和赖氨酸高于对照外,其它17种氨基酸均较对照低,但仅丝氨酸、苏氨酸和色氨酸与对照差异显著;取食KMD1后3天,绝大多数氨基酸含量的变化与取食KMD1后0天的趋势一致,除丙氨酸和色氨酸比对照稍低,其它18种氨基酸均高于对照,其中精氨酸、缬氨酸差异显著,丝氨酸差异达极显著。

On the basis of the instrumentation to study intact-receptor based on maxillary palp of cockroach Periplaneta Americana, a thermoplate was designed for constant temperature control during the experiment and a new electrode holder was also designed for improving reproducibility.

依响应频率将氨基酸分成了三类:响应频率最高的第一类为天冬氨酸、谷氨酸、甘氨酸和脯氨酸,其次为第二类包括组氨酸、精氨酸和苯丙氨酸,第三类为没有响应信号的包括丙氨酸、色氨酸、酪氨酸和苏氨酸等。

Results\ The levels of some essential amino acids like valine,leucine and histidine were significantly lower than those of normal controls.

结果 血透患者在透析前的必需氨基酸除苯丙氨酸外均较正常对照组降低,其中以缬氨酸、亮氨酸和组氨酸下降为显著;非必需氨基酸中精氨酸、甘氨酸和鸟氨酸明显升高;酪氨酸/苯丙氨酸和丝氨酸/甘氨酸的比值较对照组明显降低。

The cancers studied here were throat cancer, aute non- lymphoblas He leukem ia and acut lymphoblastic leukemia. The result s indicated that,(1)the levels of Leu, Lys, Ser Asn,Ile,Gly,and Thr were obviously decreased whea lse the levels of orn, Trp evidently increased in cultural liquid of throat can cer cell line indicating that the growth of HepⅡ cell consumed 7 amino acids and releaced 2 amino acids into cultural liquid.(2)the levels of Glu, Gls, Leu, Phe, Tyr and Trp were evidently increased while the levels of Thr , His, Ala decreased in plasma from patient with ANLL. These r esults were consistent with the previous report.(3)the levels of Gly, Trp and Phe in plasma from patients with ANLL evidently de creased and the levels of Ala, His increased after therapy indicating that cance r cells were growing in an anaerobic catabolism state.

这些癌肿包括喉癌HepⅡ细胞,急性非淋巴细胞白血病和急性淋巴细胞白血病,结果表明:(1)喉癌细胞株培养过程中亮氨酸、赖氨酸、丝氨酸、天冬酰胺、异亮氨酸、甘氨酸以及苏氨酸等水平明显降低,而色氨酸水平明显增加,说明喉癌细胞的生长繁殖必须依赖以上7种氨基酸同时释放了色氨酸;(2)急性非淋巴细胞白血病患者血浆中的谷氨酸、甘氨酸、亮氨酸、苯丙氨酸、酪氨酸和色氨酸等水平明显升高,而苏氨酸、组氨酸、丙氨酸等水平明显降低,这些结果与国际报道相一致;(3)经治疗后,ANLL患者血浆中甘氨酸、色氨酸和苯丙氨酸等水平明显降低,而丙氨酸、组氨酸等水平明显升高,表明肿瘤细胞处在无氧代谢。

Xinjiang medicine's content of amino acid of fruit juice of mulberry fruit is concrete: 2,6-diaminocaproic acid 0.089, serine 0.044, histidine 0.025, glycine 0.049, ammonia 0.088, valine 0.057, 2-amino-5-guanidinovaleric acid 0.051, isoleucine 0.042, Radix Asparagi 0.068, 0.020, threonine 0.076, 0.022, 0.128, 0.069, third 0.048, benzene third 0.042 leucine glutaminic acid methionine hydroxyphenylaminopropionic acid.

新疆药桑椹果汁氨基酸含量具体为:赖氨酸0.089、丝氨酸0.044、组氨酸0.025、甘氨酸0.049、氨0.088、缬氨酸0.057、精氨酸0.051、异亮氨酸0.042、天门冬氨酸0.068、酪氨酸0.020、苏氨酸0.076、蛋氨酸0.022 、谷氨酸0.128、亮氨酸0.069、丙氨酸0.048、苯丙氨酸0.042。

In this paper, firstly using natural fatty acids including lauric acid, myristic acid, palmitic acid etc. and disproportionated rosin as starting materials, the acyl reaction of starting materials with SOCl_2 respectively, and then using Schotten-Baumann condensation: on the reaction conditions of alkalescence, condensation of acylchloride with amino acid (sarcosine, glycine, alanine etc.), and then acidification, saltation. A series of anionic surfactants of N-acyl amino: sodium N-fatty acyl sarcosinate (SFS-12, SFS-14, SFS-16); sodiun N-fatty acyl glycinate(SFG-12, SFG-14, SFG-16); sodium N-fatty acyl alaninate(SFA-12, SFA-14, SFA-16)and sodium N-disproportionated rosinoyl aminatewere prepared. During the preparation of N-acylamino acid, the reaction conditions of acylchloride with amino acid condensation were identified by optimizing the synthetic conditions of N-lauroyl sarcosine: mol ratio of amino acid to acylchloride 2:1, reaction taken place in a solvent composed by acetone/water 2:1, acylchloride and 20% NaOH were slowly added to the reaction mixture at the same time while maintaining the pH at 9~10, after completion of adding maintaining reacting for 2.5h at 25℃.

首先以月桂酸、肉豆蔻酸、棕榈酸等天然脂肪酸和歧化松香为原料,与氯化亚砜反应制得酰氯,然后采用Schotten-Baumann 缩合法路线,即在碱性条件下,酰氯和氨基酸(肌氨酸、甘氨酸、丙氨酸等)缩合,经过酸化、成盐,合成一系列氨基酸型阴离子表面活性剂:脂肪酰肌氨酸钠,即月桂酰肌氨酸钠(SFS-12)、肉豆蔻酰肌氨酸钠(SFS-14)、棕榈酰肌氨酸钠(SFS-16);脂肪酰甘氨酸钠,即月桂酰甘氨酸钠(SFG-12)、肉豆蔻酰甘氨酸钠(SFG-14)、棕榈酰甘氨酸钠(SFG-16);脂肪酰丙氨酸钠,即月桂酰丙氨酸钠(SFA-12)、肉豆蔻酰丙氨酸钠(SFA-14)、棕榈酰丙氨酸钠(SFA-16);N-歧化松香酰基氨基酸钠(sodium N-disproportionated rosinoyl aminate),即N-歧化松香酰基肌氨酸钠(sodium N-disproportionated rosinoyl sarcosinate ,简称SDRS)、N-歧化松香酰基甘氨酸钠(sodium N-disproportionated rosinoyl glycinate,简称SDRG)、N-歧化松香酰基丙氨酸钠(sodium N-disproportionated rosinoyl alaninate,简称SDRA)。

They were two new compounds, [cyclo(Pro-Val-Phe-Phe-Pro-Val-Phe-Ser-Leu),7],[2-N-(1-methoxycarbonylethyl)guanosine, 10], and eight known compounds,(p-hydroxybenzoic acid, 1),(methyl succinate, 2),(russulaceramide, 3),(phenylalanine, 4),(guanine, 5),(β-carboline, 6),(uridine, 8), and (adenosine, 9). All the compounds were isolated from Amanita exitialis for the first time.

分别为:对羟基苯甲酸(p-hydroxybenzoic acid,1)、丁二酸二甲酯(methyl succinate,2)、神经酰胺(russulaceramide,3)、苯丙氨酸(phenylalanine,4)、鸟嘌呤核苷(guanine,5)、β-咔啉(β-carboline,6)、环(脯氨酸-缬氨酸-苯丙氨酸-苯丙氨酸-脯氨酸-缬氨酸-苯丙氨酸-丝氨酸-亮氨酸) [cyclo(Pro-Val-Phe-Phe-Pro-Val-Phe-Ser-Leu),7]、尿嘧啶核苷(uridine,8)、腺嘌呤核苷(adenosine,9)、2-N-(1-甲氧羰基乙基)鸟苷[2-N-(1-Methoxycarbonylethyl)guanosine, 10]。10个化合物均为首次从致命鹅膏中分离得到,其中化合物7和10为新化合物。

Results: Good linearity was observed in 6~1512μmol.L-1 range for phenylalanine, the intra-run and inter-run imprecisions w ere 3.68%and 4.01%, the recovery rate was 98.6%; good linearity was observed in 5.4 ~1380.0μmol.L-1 range for tyrosine, the intra-run and inter-run i mp recisions were 1.87%and 2.59%, the average recovery rate was 98.9%.

酪氨酸的线性范围为5.4~1380μmol.L-1,最低检测浓度为0.7。μmol* L-1,平均回收率98.9%,批内CV为1.87%,批间CV为2.59%。健康儿童血清苯丙氨酸浓度为(61.4±10.8)μmol.L-1,血清酪氨酸浓度为(131.8±28.0)μmol.L -1。健康成人血清苯丙氨酸浓度为(69.3±8.6)μmol.L-1,血清酪氨酸浓度为(83.6±9.4)μmol.L-1.4例苯丙酮尿症患儿的血清苯丙氨酸浓度为1296~1759μmol.L-1,血清酪氨酸为29.5~53.5μmol.L-1。

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