世代

ABSTRACT It is because that the excellent acceptors for maize transgenic engineering are insufficient in our country, especially in the southwest mountain areas of china and hereditary variation regularity for the two characters such as efficiency of embryonic callus induction and number of regenerating plant (these two characters were abbreviated to the nduction efficiency and number of regenerating in the following of the paper, respectively), which hint the maize culturing capacity, is not very clear. Therefore, aiming at picking out superior acceptors, we had made systematic researches on the two characters with combing traditional quantitative-character genetic analyzing methods such as single-factor genetic mating design, diallel crossing genetic design, genetic effect analyzing method and the modern molecular locating method such as QTLs'. The main results are followed.(1) 50 superior inbred lines and about 30 crosses in our country, especially in the southwest of China were used for identifying and selecting the superior genotypes in the above two investigated characters under the same culturing condition in 2000 and 2001. There was very significant difference among the genotypes in the both characters. But the two characters were not certainly related. Some genotypes such as 18-599 and 18-599 were very good in them. For some ones such as zong 31, induction was higher than 18-599 and 18-599 in the efficiency, but it was only 1/3 to the later in regenerating number. In some genotypes such as S37, R08, R15, P138, A318, induction efficiency was just about 3% and scarcely any regenerating plants were got. On the whole, hybrids acted better than inbreeds in the both characters.(2) Two kind of inbreeds were selected as parents of the Griffing's method 1. 18-599 and 18-599 and the inbred line zong 31 are one kind because they are not only superior in the characters of maize cross breeding, such as CA, resistance to disease and the important agricultural characters, but also excellent in transformation characters as the induction and regeneration.

针对我国、特别是西南山地所需玉米转基因工程育种优良受体极为匮乏和反应玉米幼胚培养能力的2个主要性状，即玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数的遗传变异规律十分不清楚的实际情况，本研究从筛选玉米转基因工程所需要的优良受体入手，采用单因素遗传交配设计、双列杂交遗传交配设计、世代基因效应等传统数量性状分析方法，以及现代分子标记定位主效QTL分析方法，对玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个性状进行了较为系统的分析研究，取得以下主要研究结果：(1)于2000年和2001年通过对我国、特别是西南地区近50份优良自交系和近30个杂交组合，在相同培养条件下，对幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个幼胚培养能力性状进行了筛选与鉴定，发现玉米不同基因型具有完全不同的幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数，但幼胚培养胚性愈伤组织诱导率与胚性愈伤组织绿苗发生数并不具有必然的相关关系，有的基因型，如自交系18-599和18-599在胚性愈伤组织诱导率和愈伤组织绿苗发生数等2个性状都表现相当优异；有的基因型，如自交系综31，仅幼胚培养胚性愈伤组织诱导率性状表现高于19-599和18-599，但在胚性愈伤组织绿苗发生数这一性状则与它们有相当大的差距，仅为19-599和18-599的1/3左右；有的基因型，如S37、R08、R15、P138、A318等玉米自交系不仅幼胚培养胚性愈伤组织诱导率很低，平均仅在3％左右，而且胚性愈伤组织绿苗发生数表现也很差，基本上没有分化成苗。

Lord, Your Holy Spirit indwells us and we are Your tabernacle in this age.

祷告 主啊，您的圣灵住在我们堶情A我们就是您在这世代的居所。

That's the root that mythology be iterated constantly.

这也就是神话不断为人类世代重述的根源。

November 21, 2005 - Every few years we get a new generation of consoles fumbling their way into our lives to lusty shouts that PC gaming is dead. No matter how loud the death knells peal, they're going to fall on some deaf ears… like ours.

2005年11月21日几乎是每隔数年，都会有新的次世代游戏机进入我们的生活，并且精力充分的狂喊着电脑游戏已经逝世亡的狂言，然无论那狂言尔带来的丧钟敲的多么隆隆作响，也只不过是给聋子听的。。。

Now, living in the Kanas Lake, a primitive tribal, they Hillwood home to grazing, hunting living, knifing to the mountain pine huts erected, take off the thick skins to withstand cold, the use of primitive cooking Modulation a fragrant milk wine, and they do not alien marriages, and others struggle, a self-proclaimed them to Tuva people to their own ancestors of Genghis Khan, the old saying that the names of their ancestors remember every Tuva is the responsibility of descendants of people, they leisurely generations live in this Shiwaitaowan Kanas, known as the forest people.

现在，在喀纳斯湖畔居住着一个原始部落，他们以山林为家，以放牧、狩猎为生，砍来山上的松木搭建起木屋，剥下厚厚的兽皮抵御严寒，使用原始的炊具调制出香喷喷的奶酒，他们不与外族通婚，不与他人争斗，他们以图瓦人自居，以成吉思汗为自己的先祖，古训说牢记祖先的名字是每一个图瓦人子孙的责任，他们世代悠闲地生活在喀纳斯这片世外桃园中，被称做&林中百姓&。

The child must kneel down to adult kowtows, thanks adult they the small generation.

孩子必须跪下来到成人叩头，感谢成人他们小世代。

For instance, in old Tibet, over 95 percent of Tibetans, from generation to generation, were serfs attached to officials, nobles and lamaseries.

例如，在旧西藏，95%以上的藏族人民是世代人身依附于官家、贵族和寺庙的农奴。

Fixed-line networks are poor or non-existent in many developing countries, so people have leapfrogged straight to mobile phones instead.

在许多发展中国家，固话网络发展落后甚至就没有。正因为如此，这些国家的人们便直接&跑步进入&移动电话世代。

Poets of the New Generation, quick to respond to the change of times, launched poets' clubs, published anthologies of verse and issued manifestoes.

台湾新世代诗人感应时代变化，创办诗社，出版诗刊，发表宣言，扮演着重要角色。

The demonstrations on that day were some of the youngest and most militant workers' demonstrations for at least a generation.

游行示威，这一天被一些年轻的和最激进的工人示威，在至少一个世代。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐