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Glycolate oxidase (EC 1. 1. 3. 1, GO) was purified by 137. 4 fold from leaves of Brassica campestris L. ssp. chinensis var. utilis and the specific activity attained 130.9 U/mg. protein. Chromatography of purified GO through Sephadex G-200 showed two symmetric peaks of both protein and activity, which overlapped fully with each other. Further with electrophoresis on agrose plate, a protein spot and an activity spot were detected in electrophoretogram after respective staining, and the spot of protein covered that of activity, which indicated that the purified enzyme was of a high quality.

从菜心叶片中纯化了乙醇酸氧化酶,比活性达130.9 U/mg,纯化倍数为137.4倍;纯化酶经Sephadex G-200凝胶过滤显现单一而对称的蛋白吸收峰和活性峰,两者完全重叠;进一步经pH 8.3缓冲系统的琼脂糖平板电泳、染色,各在负极端出现一个活性斑点和蛋白染色斑点,两者的位置完全对应;表明纯化的菜心叶片乙醇酸氧化酶已达到较高的纯度。

The results show that the density of gritstone is decreased gradually as the temperature increasing; so that it can be ignored; the longitudinal wave velocity is linearly decreased when the temperature increasing, and decrease rate of it follows the law of conic; R is one temperature point, which makes the law of tensile strength of gritstone after high temperature follows the law of parabola; and the maximal tensile strength appears when the operative temperature is R. After the temperature is higher than 700 ℃, tensile strength is small and change range is small when the temperature is increased.

结果表明,高温后粗砂岩的密度随试验温度的升高减小幅度很小,几乎可忽略不记;高温后试件中纵波波速的传播随温度升高基本呈线性降低,而减小率呈二次曲线规律变化;存在一个温度点R,使高温后试件的抗拉强度呈开口向下抛物线关系变化,在R温度作用后试件抗拉强度达到最大;当试验温度大于700 ℃后,试件的抗拉强度变得很小,当温度升高时变化幅度也很小。

he fact that the sales in the Mexican and Guatemalan markets were at different levels of trade was apparent from the application itself, and an unbiased and objective investigating authority should have recognized this fact without the need for it to be pointed out.

墨西哥和危地马拉销售市场处于不同的贸易水平这一事实在申请中是明显的,因而公平客观的调查当局应该认识到这一事实,不需要特别指出。

The numerical result shows that the contributions from the interface optical phonon mode with higher frequency and the LO-like half space mode to the binding energy and the Stark energy shift are important and obviously increase with increasing hydrostatic pressure, whereas the interface optical phonon mode with lower frequency and the TO-like half space mode are extremely small and are insensitive to the impurity position and hydrostatic pressure.

数值计算结果表明,高频支界面声子模和类LO半空间声子模对于结合能和斯塔克能移的影响是主要的,随压力的增加而显著增大,而低频支界面声子模和类TO声子模的作用则很小,对于杂质位置和流体静压力的变化不敏感。

English immersion is hands-down the best way to learn English, but going abroad for an English course is too expensive for most people.

有趣的游戏和主题,再加上"随时随地说英语"的生活型态,也藉由和外籍英语教师之不间断的互动,学生将会在许多具体实际的情况下学到的英语,进一步的学习使用它们。

A simple herniotomy is effective and safe in patient ≤18 years old with a primary inguinal hernia. Posterior wall enhancement is not necessary in patients ≤18 years old with a primary inguinal hernia.

以標准之疝气袋高位结扎方式修补小於18岁之原发性腹股沟疝气是安全有效的手术,对於小於18岁之原发性腹股沟疝气没有必要进行后腹壁修补术。

Aimed at the calculation complexity of Newton iterative method for solving nonlinear equation,a new iterative method with higher convergence rate and without derivative calculation is presented in this paper.Its convergence rate p is 1.839 and it doesn't need to calculate derivative.

为解决Newton迭代法求非线性方程数值解时必须提供一阶导数值的问题,提出了一个新的迭代方法,该方法不需提供导数值而只需计算函数值,具有p=1.839的收敛阶,因而是一个收敛速度快不需要计算导数值的迭代方法。

At present, Chinas main method control classical swine fever is vaccine injection, that artificialactive immunization, As for it is no final conclusion that the difference of domestic CSF immuneeffects with advanced immunization and ordinary routine immunization. And it will take enormousmanpower and resources for farms to take advanced immunization, and there is a possibility of certainanaphylactic response. It is not easy extensive, the immune programme of each hoggery is difference.

目前我国防治猪瘟主要采取注射猪瘟疫苗的人工主动免疫方法,由于国内对猪瘟超前免疫和普通常规免疫两种方法在免疫效果上的差别没有定论,养殖场采取超前免疫措施需要大量的人力及物力,存在一定的过敏反应,不容易广泛开展,因此各个养猪场所采取的猪瘟免疫程序各不相同。

All of the patients were very sick, homebound, and could not tolerate any meaningful activity, he said.

所有病患病情都非常危急、居家无法忍受任何有意义的活动。

In this paper, the following statements were proved: is chaintransitive if and only if is chaintransitive; if a homeomorphism is a Lipschitz map, then has the average shadowing property if and only if has the average shadowing property.

设是紧致度量空间,:X→X是连续的,n为任一给定的正整数,证明了:是链可迁的当仅当是链可迁的;若同胚是Lipschitz映射,则有平均跟踪性当仅当有平均跟踪性。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力