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The phytoplasmas diseases were investigated in 30 counties and cities of 10 areas in Yunnan Province. There are 12 phytoplasma diseases, including paulownia witches-broom,chinaberry witches-broom, cactus witches-broom, christmas cactus witches-broom, orange little leaf, petunia flat stem and so on were indentified using PCR with universal primers, which amplified phytoplasma 16SrRNA gene and/or ribosomal protein genes. So the kind of phytoplasma diseases in Yunnnan Province were understood. Identified the characteristic of 16SrRNA and rp gene of phytoplasma and the kind, classification and genitic relativity of these strains using RFLP, clonging and sequence analysis (nearly 20 full-length sequences of phytoplasma 16SrRNA and/or rp gene were submitted in GenBank ); The phytoplasma strains isolated from herbage plants were conserved in storeroom. 9 articles were published in the key journals of China and 1 technique invention patent was applied. The net-page about phytoplasma disease was established. 4 graduate student were bringed up.

对云南省10个地区的30余个县市地区进行了植原体病害的调查采集工作;采用植原体16SrRNA基因通用引物(R16mF2/R16mR1及R16F2/R16R2)和/或植原体核糖体蛋白基因引物(rpF1/R1)对所采集到的病害标样总DNA进行PCR扩增,根据植原体特异扩增条带的出现,共鉴定出泡桐丛枝、苦楝丛枝、仙人掌丛枝、蟹爪兰丛枝、柑桔小叶、矮牵牛扁茎等12种植原体病害,初步明确了云南省植原体病害的种类;通过RFLP技术、克隆及测序技术获得其16SrRNA及部分核糖体蛋白基因的近全长序列(已在GenBank中共登录近20余个相关序列),通过序列比较分析,明确了上述植原体株系中这两个基因的特征并确定了云南省植原体株系的种类、分类地位及遗传相关性;对一些发生在草本植物上的植原体进行了株系保存;在国内核心期刊及一般期刊发表研究论文 9 篇,申请技术发明专利1项;初步建立了有关植原体病害的网页;培养研究生4名。

Chiron filed for several patents on the virus and its diagnosis.

凯龙公司提交了对这种病毒的多项专利和诊断方法。

In 2004 Chiron held 100 patents in 20 countries related to hepatitis C and had successfully sued many companies for infringement.

2004年,凯龙公司已经持有100项与丙型肝炎相关的专利,并成功地起诉了多家侵害其权利的公司。

Nanjing University of Science and Technology is the earliest researcher in researching chlorine dioxide in China.

南京理工大学是国内最早开发ClO2产品的单位之一,也是获得ClO2研究成果和专利最多的单位之一。

However, in the process of patent retrieval, it has been a choke point to grasp with more exact keywords.

虽然文献上,有少数研究利用Text Mining的作法,来改善专利检索的问题。

Because the dimeric form is the active form to exert its biological activities, many trials failed to express TGF β s in prokaryotic systems because of their complex folding manner and disulfide patterns. In 1991 Cerletti and coworkers from Ciba-Geigy Corporation in Switzerland successfully finished the functional expression of human TGF β 2 in bacteria, but the details are protected by the patent.

由于双体是其发挥生物活性的形式,TGF β复杂的折叠方式和二硫键配对使其早期原核基因工程研究都以失败而告终。1991年,瑞士Ciba-Geigy公司的Cerletti等成功完成了人TGFβ2基因的细菌表达,但其技术细节被专利加以保护。

Circuity of Action is a long-standing problem in patent invalidity declaratory judgmentlitigation in China.

我国专利无效诉讼领域长期存在"循环诉讼"的问题。

At present, Beijing Huayi puts its main strength on China City Blue Page, a Beijing-based project aiming at whole China. In addition, we have successfully applied patent for China City Blue Page Public Information Free Inquiry Machine and registered China City Blue Page brand. Now Beijing Huayi is a forerunner in IT industry in China.

目前我公司主营项目《中国城市蓝页》立足北京积极向全国拓展,申请了"中国城市蓝页公众信息免费查询机"专利,同时注册了"中国城市蓝页"商标,填补我国目前IT行业一项空白。

In May it said it was entering licensing deals with Claris LifeSciences and Aurobindo, both Indian generics firms.

今年5月,该公司与印度的两家非专利药品制造商Claris Lifesciences与Auro-bindo达成经销权协议。

Patent of chilling effects clearinghouse is maintained by University of Maine, School of Law.

冷冻效果交易所研究主题中的专利研究是由缅因州大学法学院主办的。

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