与

The rhizosphere soil microorganism and rhizosphere soil enzyme activity werepositive correlated with each other.Except aerobic bacteria and actinomycetes andfungi did not achieve significant or extreme significant correlation with urease,theother factors all achieved significant or extreme significantcorrelation.Besides,through a correlation analysis between rhizosphere agrobiologyand rhizosphere soil nutrient,except rapidly available K was negative correlation with urease and catalase,the other agrobiology factors were positive correlation with soilnutrient, aerobic bacteria and sucrase and catalase were extreme significantcorrelation with alkaline hydrolytic N and organism matter and rapidly available P;actinomycetes was extreme significant correlation with all soil nutrient factors;fungiwas extreme significant correlation with alkaline hydrolytic N and organism matterand rapidly available P, significant correlation with rapidly available K;urease wasextreme significant correlation with alkaline hydrolytic N, significant correlation withrapidly available P.

本试验所测定的根系土壤微生物与根系土壤酶活性间均呈正相关，除了好、气性细菌、放线菌、真菌与脲酶间未达到显著或极显著相关外，其余因子间均呈显著或极显著相关；此外，通过根系土壤生物学因子与根系土壤养分相关分析，发现除了速效K与脲酶、过氧化氢酶呈负相关外，其余生物因子与土壤养分因子间均呈正相关，其中，好气性细菌、蔗糖酶、过氧化氢酶与水解N、有机质和速效P极显著相关；放线菌与所有养分因子间均极显著相关；真菌与水解N、有机质和速效P极显著相关，与速效K显著相关；脲酶与水解N极显著相关，与速效P显著相关。

Scientific and theoretical activities of the ATC are supposed to include: investigation, generalization and analysis of countersabotage and antiterrorist actions in local wars and armed conflicts and elaboration of respective proposals and recommendations for commanders, staffs and armies; development of trends aimed at perfection and improvement of operative and tactical forms and methods of the struggle against subversive and terrorist formations, combat planning and control techniques, control system and all-out support development; drafting of manuals, handbooks and instructions, soldiers' handling guides, education materials related to the given subjects.

反恐怖中心的科学和理论活动应该包括：调查、分析、总结局部战争与武装冲突中的反破坏与反恐怖行动，为指挥官研究制定相应的建议；着眼于完善与改进对抗破坏与恐怖编队作战与战术的样式与方法、战斗计划与控制措施、控制系统与各种保障，研究破坏与恐怖活动的发展趋势；起草制定相关的指南、手册与说明书、士兵指南、教育训练材料。

The full stress optimizes the application in connecting a design of greenhouse with; Even the experimental study of the only warm environment of winter of a plastic greenhouse; Intensify the current situations and trends of raising pig"s craft development; The scale beasts and birds cultivate the technical research progress of the environmental project; Effective way utilized in recycle treatment of the beasts and birds offal; The nourishing oral liquid culture current situation of Japan and new technology; Engineering of animal husbandry of our country and industrialized development are 20 years; Technological economic analysis that heat energy retrieves in winter of chicken coop of North China; Connect large-scaly with the design and test of the natural day-lighting of a chicken coop; Even the experiment research of the environment of summer of a chicken coop; The pig house cools the current situation of technological application study in summer; Application and design of the vertical ventilating system of pig house; The evaporation of agricultural building cools the current situation and prospect of technological study and application; Of our country the South open to cool the question summer in chicken coop; Vertical air current organization that ventilated of chicken coop; Innovation and Development of China"s Animal Husbandry project Science and Technology; The pig farm of scale is raised in the shed and raised the clean production technology mode scattered to study; the Project of the cow is raised in the shed in the scattered fence; Give up and raise sheeps new craft mode to visit for the first time in scale; The piglet keeps the case warm one degree of characteristics of playground of body temperature is studied; Structure optimization of the joining type pig house of roof truss of light steel; Lose electrical power such nearly one hundred as the impact on airtight type hot wet environment of pig house ,etc.

满应力优化在连栋温室设计中的应用；连栋塑料温室冬季光温环境的试验研究；集约化养猪工艺发展的现状与趋势；规模化畜禽养殖环境工程技术研究进展；畜禽废弃物的资源化利用的有效途径；日本的营养液栽培现状及其新技术；我国畜牧工程技术与产业化发展20年；华北地区鸡舍冬季热能回收的技术经济分析；大型连栋鸡舍自然采光的设计与测试；连栋鸡舍夏季环境的实验研究；猪舍夏季降温技术应用研究现状；猪舍纵向通风系统的应用与设计；农业建筑蒸发降温技术研究与应用的现状与展望；我国南方开放式鸡舍的夏季降温问题；鸡舍纵向通风的气流组织；中国畜牧工程科学技术的创新与发展；规模化猪场舍饲散养清洁生产工艺模式研究；奶牛舍饲散栏饲养工程工艺研究；规模化舍饲养羊新工艺模式初探；仔猪保温箱体温度场特性研究；拱型轻钢屋架猪舍的结构优化；夏季停电对密闭式猪舍热湿环境的影响等近百篇。专著与教材：《家畜环境与设施》《畜牧工程——科技创新与发展》《可食用观赏植物》《园艺设施学》《林果花菜设施快速栽培技术》《农业生物环境与能源工程》《居室花卉装饰》《现代温室技术》《中国养鸡学》等。

The quality of interior environment in nursing home residents will become one of the attention-getting subjects due to the gradual increase of aging populations This paper directs at the research of nursing home's interior environment factors in Tainan We use the standard of international practice to analyze the intrinsical data of environmental monitoring and establish the characteristics which from environmental physical reaction of nursing home The research items are air environments (PM10 CO CO2 formaldehyde TVOC) temperate environments and light environment (illumination average brightness daylight factor) etc There are the inductive conclusions of this research: 1 In basic medicine the clinical diagnosis means the theory and technique about physical examination with patients The definition of clinical diagnosis in architecture should be the diagnosis of building preserve and improve the feasible way to extend building's service life and assure the residents of the healthy quality of interior environment The object of clinical diagnosis in architecture is not only to ensure the use of structure and environment quality but also keep the structure and environment quality in sustainable; therefore creating an efficient system of preserving plan is needed 2 It is an accurate and technical circumstance which about improving remedying and reconstructing the problems of structure space This circumstance includes finding questions ensuring causation ascertaining ultimate strength evaluating environment quality estimating the necessary of restoration and implementing on a suitable way Furthermore when reconstructing and ameliorating the appearance of structure space it should be respected and the memories of it should be preserved 3 In clinical diagnosis of environment quality of space the question of physical environment and the deficiencies of structure must be separated The structure space of construction and the high quality of space should be inspected strictly then observed the crucial reason that can find out any invisible and hidden causes The diagnosis of construction is to observe the reason of destroyed structure The environmental diagnosis of indoor and outdoor is to find out the crucial reason that influences health 4 In order to decrease the iterant problems the construction and environment of space should be diagnosed However the destruction by natural strength and artificial using by human are important causes that can affect the service life of construction Searching the cause of disease just like curing the patient which includes inspection diagnosis cure and prevention We tested the thermal conductivity of siding material in this research and then found out the roof east vertical face and west vertical face have most radiant heat especially the construction of RC The characteristics of opaquely outer casing have relations with the thermal conductivity of wall to wiz the ability of heat insulation Therefore increasing the efficiency of heat insulation and decreasing the endoergic factor of insolation are very important to temperate environments

而随著国内老年人人口逐渐增加，逐渐迈向高龄化之同时，对於高龄者使用空间之室内环境品质相关议题之探讨，亦成为未来我们需加以关注之议题之一。主要为针对台南某安养中心室内环境因子进行检测调查工作，利用现场室内环境测定以取得有效的实测数据；再藉由汇整相关国际评估基准，并进行实测数据比对分析，建立老人安养中心对物理环境反应之特性。并以前期相关研究文献回顾与老人安养中心实测案例现场的实验，来了解不同空间物理环境特性之影响，并将结果回馈实际建筑及室内设计之用。调查工作项目包含：空气环境之PM10、CO、CO2、甲醛、TVOC；温热环境之室内温度、相对湿度、人体PMV、PPD；光环境之照度、均齐度、昼光率等因子调查。本研究结果可归纳以下几点结论： 1 所谓临床诊断医学乃相对於基础医学而言，是指实际与病人接触的医疗及护理行为中牵涉到的理论和技术；而建筑的临床诊断的定义应为建筑本体与室内外诊断、维护及改善所有可行的方式来延长建筑物的寿命，并确保人与室内环境空间品质的健康。建筑临床诊断目的为确保结构体与环境品质能继续使用，而能维持所需要的效能及承受能力。一个有效的维护计画体系是必须建立的 2 在建筑空间之问题改善、治疗与修复执行中，是一个精确与技术性的事项，其中包含找出问题、确定原因、评估结构应力强度、评估环境品质、评估修复与改善必要性、选择及执行一个适当的过程。其改善与修复也必须尊重建筑空间原有色彩与形貌，并保留原有记忆及文化。 3 在临床诊断中空间环境品质中，物理性环境问题与结构构件缺陷必须分别判断，诊断建筑空间之结构与空间舒适品质必须详细观察其症结性问题，才能发现任何隐藏及有潜伏性缺陷的原因。建筑本体诊断是观察出建筑本体之破坏原因，而是内外空间环境诊断目的在於的找出影响健康因素之症结性问题。 4 建筑本体与空间环境之预防评估目的为降低与抑制问题的再发生。然而，自然力的破坏与人为使用因素皆是影响建筑生命期之主要因素，找出病源问题就如同医生对病人的处理，包含了侦查、诊断、治疗以及预防。 5 本研究以外墙材料热传导率测试结果发现，屋面与东西向立面是承受辐射热最多的地方，尤以 RC 的建筑更甚，不透明部分的外壳能特性主要与壁体的热传透率即隔热能力有关，但是承受来自日射的吸热影响，因此增加隔热性能与降低日射吸热因子是对温热环境是非常重要的。

Programming, Discrete Mathematics, Data Structures and Algorithms, Introduction to the Design and Analysis of Algorithms, Operating Systems, Compiler Principles and Practice, Computer Networks, Computer System Architecture, Embedded Systems and Applications, Database Systems and Applications, Formal Language and Automata, Software Engineering, Artificial Intelligence, Introduction to Computer Graphics, Software System Design, Software Project Management, etc.

主干课程：算法分析与设计、面向对象技术与应用、软件项目管理、软件体系结构、软件过程改进、计算机网络技术、网络与信息安全技术、软件测试技术、信息系统分析与设计、软件度量技术、数据库管理技术、软件平台与中间件技术、数据仓库与数据挖掘、软件需求工程、计算机图形学等。研究方向：软件工程与管理、软件理论与系统、信息系统与工程、计算机图形学与辅助设计。

In the end, there is a brief conclusion . The conclusion of this passage mainly includes: The listed companies have obvious preference for stock right financing, and the financing order is stock right financing, the short-term debt, endogenous financing, the long-term debt, which differs from that of western countries; Financing structure is characterized by comparatively lower asset-liability rate, higher floating debt and lower long-term debt; The governance structure is irrational, and the shareholders" meeting perform practically no function. In the meanwhile, board of director structure is unreasonable, which is highly coincident with the manager layer. Board of supervisors" independent character is poor, while manager layer is lacking in restraints and supervision; The interdynamic

本文的结论：上市公司有明显的股权融资偏好，融资顺序为股权融资、短期负债、内源融资、长期负债，这与西方国家上市公司的融资顺序不同；融资结构具有资产负债率相对较低，流动负债偏高、长期负债偏低的特征；治理结构不合理，股东大会形同虚设、董事会结构不合理、董事会与经理层高度重合、监事会独立性不强、经理层缺乏约束与监督；治理结构与融资策略选择的互动关系不强；独立董事比例与资产负债率正相关，工业、公用事业与资产负债率负相关，商业与流动负债率正相关，第一大股东持股比例与流动负债率负相关，第一大股东持股比例与长期负债率正相关，执行董事比例、工业、商业与长期负债率负相关。

During this course, there are contradictions between modern and postmodern, struggle between rationalism and sensualism, antinomy between structuralism and deconstructionism, dissension between homogeneity and heterogeneity... These contradictions and dissension are the embodiment of transformation from industrial society to post-industrial society in the cultural domain. It provides references for the socialist culture construction of our country.

从文化学层面分析，它充斥了文化同质性与文化异质性的对抗与消解，文化高雅性与文化通俗性的对峙与融合，文化工具性与文化价值性的斗争与合流；从哲学层面分析，它又体现了文化一元主义与多元主义的对立与统一，文化结构主义与文化解构主义的交锋与并立，文化绝对主义与文化相对主义的冲突与共存，这一切集中反映了西方在由工业社会向后工业社会转变过程中文化的特有矛盾和状态，也为我国社会主义文化建设提供了借鉴和参考的价值。

Baby girl You know my situation And sometimes I know you get impatient But you dont put to on a show to get ovations Take it to court and go through litagations And I respect ya gangsta Treat you like a princess And put something on your neck to thank ya Shes my pinch hitta When the startin lineup aint playin right I come off the bench wit her It might sound like im gassin ya But it took time to get from the back seat to the passenger We been creepin and sneakin Just to keep it from leakin We so deep in our freakin That we dont sleep on the weekend Wifey A little bit uptight Wonderin why he comin home in the middle of the night Itll be alright if yall bump heads itll be a fight But i said itll be alright Come on I really wanna be with you be with you But I gotta be real with you real with you I cant leave you alone lone And I know I live and grown But i cant let ya go Your the one i want in my life want in my life Already got a wife got a wife Cant leave you alone lone And I know I live and grown But I cant let ya go You aint ever step out of line Or get out a pocket So i made sure canary sent out your locket To protect you, ill get out and cock it And you know the barrel of my gun is big enough to spit out a rocket Oh, you gonna play dumb if cops do come through I gotta keep the top up if my drop do come through But i know the boutiques and the shops you run through So i cop her one, and cop you one too You always get a daily page, weekly ring Plus you aint too shy to do them freaky things I aint gotta put a band on your finger I worry about you tellin the whole world im your new allen springer At first you were somethin i denied Something I would slide Just do somethin in the ride But shorty Theres something that you provide Cause the entre pain is good without somethin on the side You know I really wanna be with you be with you But I gotta be real with you real with you I cant leave you alone lone And I know I live and grown But i cant let ya go Your the one i want in my life want in my life Already got a wife got a wife Cant leave you alone lone And I know I live and grown But I cant let ya go Uh oh, i might be leavin the earth soon My girl gonna kill me if she smells the scent of your perfume Its gonna be a clip towards if I go back With stains of your lip gloss on my throwback She wont care if im a platinum rapper If she catch me with an empty magnum wrapper So keep it on the down low call the carsely You seen what happened with Mr. Big and R Kelly You know I get from you Anytime this chick is there for you Feelings imma share wit you Which makes it a Lil Mo clear for you x2 I really wanna be with you be with you But I gotta be real with you real with you

女婴 你知道我的处境有时候，我知道你不耐烦但你dont提出对表演获得掌声是否向法院经过litagations 我就帮遐把你像公主并提出一些对你的脖子谢遐畲族我捏hitta 当做起阵容并不是playin权我来替补，她风趣它听起来像即时几率遐但花时间从后座向乘客我们已经和creepin sneakin 刚刚把它从渗漏我们这么深的年freakin 我们dont睡上周末 wifey 有点紧张 wonderin为什么他搭档的家中半夜 itll予以好吧，如果男子跳车元首itll被扑灭但我说itll予以好吗来我真想与你与你但我干真正与你真正与你着我离开你独自独行我知道我的生活和成长但我让着去遐你的一个，我想在我的生命中希望我的人生已有一个妻子了妻子着离开你独自独行我知道我的生活和成长但我让着去遐你是不是有史以来走出线还是走出了口袋所以，我作了肯定的金丝雀寄出你的头发为了保护你，生病走出它公鸡你知道枪管的枪，我是足够大吐出火箭哦，你再拖扮演哑巴，如果警察不来，通过我干随时补足，如果我落不来通过但我知道，专卖店和商铺，你流经所以她的缔约方之一，并缔约方之一，你太你总是得到页每日，每周响另外你是不是太害羞做事情freaky 我并不是干把带手指我担心你tellin全世界即时你的新阿伦螺旋藻起初你somethin本人否认我将下滑刚做somethin在坐但shorty 照片theres东西，你提供造成entre痛是好的无somethin对方你知道我真想与你与你但我干真正与你真正与你着我离开你独自独行我知道我的生活和成长但我让着去遐你的一个，我想在我的生命中希望我的人生已有一个妻子了妻子着离开你独自独行我知道我的生活和成长但我让着去遐呵哦，我可寄至地球快我的女孩再拖杀了我，如果她的气味香，你的香水其再拖剪辑走向，如果我回去污渍与你唇光泽我倒退她で照顾，如果即时白金唱片如果她追上我用空枕包装所以保持对低下来致电carsely 你看到发生了什么大先生和r凯利你知道我从你随时这只小鸡有你投书感受分享您智慧这使它成为莫律清楚，你χ2 我真想与你与你但我干真正与你真正与你但我干真正与你真正与你着我离开你独自独行我知道我的生活和成长但我让着去遐你的一个，我想在我的生命中希望我的人生已有一个妻子了妻子着离开你独自独行我知道我的生活和成长但我让着去遐我真想与你与你但我干真正与你真正与你着我离开你独自独行我知道我的生活和成长但我让着去遐你的一个，我想在我的生命中希望我的人生已有一个妻子了妻子着离开你独自独行我知道我的生活和成长但我让着去遐

Trade, consumer electronic products trade, power station equipment trade. Trade in services and industrial investment areas, Afro-Asian International Group, its frequent use of foreign exchanges, with the civil business built up good cooperation relationship and personal connections as well as the perfect professional services, engage in exchange of cultural performances, exhibition services, Business States to study business travel, project management consulting, the World Bank Asian Development Bank, African Development Bank and other international financial institutions, project contracting loans, government grants foreign aid project contracting, the introduction of U.S. venture capital patent production of civilian sensors, and the International Geothermal Association Cooperation in the geothermal resources development and utilization, engineering supervision and six Indian companies for electricity power station in India Highway Advisory sewage treatment project tender, with the Indian national and local chambers of commerce to engage in trade consultation, and cooperation between the Confederation of Indian Industry Exhibition Services, and the Federation of Indian mining and mineral owners Orissa iron ore trade cooperation between the agents, and Iraq Alebier Construction Group cooperation in the cement exports, with the London Metal Exchange members of discarded rail trade cooperation with Ghana Minerals Association and the Sierra Leone Diamond Research Center, Shajin agreement agent and the diamond trade, and the Congo and Zambia Copper Company Acting copper and electrolytic copper trade, Kazakhstan, Nigeria and Russia Acting Saudi oil trade in Malaysia Indonesia, and Saudi Arabia Amorco, Russia Trasnneft, Rusneft, Gasplome, Nigeria NPOC, Kazakhstan OSCJ, Iran's NOIC, Malaysian Petronas, Venezuela VNOC, Cyprus HANS OIL maintain good relations of cooperation, and nearly 100 more than the global oil agent and the channel exchanges sharing resources ......

在服务贸易和实业投资领域，亚非国际集团利用其频繁的对外交流，与各国政府民间商业界建立起来的良好合作关系和人脉关系以及完美的专业服务，从事文化演出交流活动、会展服务，赴各国商务考察商务旅游，项目管理咨询，世界银行亚洲开发银行非洲开发银行等国际金融机构工程承包贷款项目，政府赠款援外项目工程承包，引入美国风险投资专利生产民用传感器，与国际地热协会理事合作进行地热资源的开发和利用，与印度六家工程监理公司合作进行印度电力电站高速公路污水处理项目的咨询招标，与印度国家和地方商会合作从事贸易咨询，与印度工业联合会合作进行会展服务，与印度矿业联合会以及奥里萨邦矿业业主合作进行铁矿石贸易代理，与伊拉克阿勒比尔建工集团合作从事水泥建材出口，与英国伦敦金属交易所成员合作进行废旧钢轨贸易，与加纳矿产协会以及塞拉利昂钻石研究中心协议代理沙金和钻石贸易，与刚果赞比亚铜业公司代理铜矿和电解铜贸易，代理沙特尼日利亚俄罗斯哈萨克斯坦马来西亚印尼原油贸易，与沙特Amorco，俄罗斯Trasnneft， Rusneft， Gasplome，尼日利亚NPOC，哈萨克斯坦OSCJ，伊朗NOIC，马来西亚Petronas，委内瑞拉VNOC，塞浦路斯HANS OIL 保持良好合作关系，与全球近100多家石油代理商和渠道商往来密切资源共享。。。。。。

The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失