与...连接

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

Data transmitted by AD7714 will be delivered to P1 of 89S52 through Din and Dout and will be settled by 89S52.The data corrected will be showed at keyboard and demonstration unit.

重点在于前端的温度传感与变送单元的设计，包括热电阻的选择问题，热电阻阻值的测定，精密桥式电阻的连接方式，滤波器的选择问题，系统稳压电源的设计和AD7714与积分式AD转换器的比较等问题。

Grounding with a wire connected to ERTH is specially important, if not the printer might be damaged by spikes of static electricity, also the please pay attention that the material to write on is not charged with static electricity, also conveyors can easily be charged with thousands of volts of static electricity, this will damage the controller, so please remove all static electricity by sufficient grounding and correct termination of all potentials.

用电线与地面连接极其重要。如果喷印机没有接地，静电的调试脉冲就会损坏喷印机。另外，还应注意填写的资料与静电不一致，数千伏特的静电给传送机充电，导致控制器受损。因此，请通过充分的接地，改正所有电压的中止，去除所有的静电。

In Gen., iii, 20, it is connected etymologically with the verb meaning "to live":"And Adam called the name of his wife Eve : because she was the mother of all the living".

在将军，三， 20日，它是连接词源与动词，意思是&住&：&与亚当所谓的名义，他的妻子除夕：因为她是母亲的全部生活&。

Firstly, the paper, combining the characteristic of synchronous pulse bursts and inhibition with the modified PCNN model, presents a way of finding the foveation points in the images adaptively and effectively, and simulates the human vision system. Secondly, PCNN is extended to PCNNs, based on the properties of information couple and transmission, an algorithm that is used to fuse images of the same target got by several sensors to an image is presented to simulate the human vision system. Thirdly, combining the properties of synchronous pulse bursts, capture, and transmission and competition of waves, the paper presents two ways of classification, one is an algorithm based on the properties of neuron to capture and inhibit to classify the data taking on any complex unlinear distribution robustly, the other is based on the restricted distance and modified of the former to remove the influence of inferior samples in classification; Finally, based on the accumulative difference pictures, and the forming and transmission of PCNN wave, selecting and controlling the direction of autowave by connecting the neighbouring neurons selectively, the paper presents a way to simulate the tracks of moving object and detect the moving direction.

首先结合PCNN的同步脉冲发放和侧抑制特性，提出了基于改进型PCNN的图像凹点检测算法，该算法是一种自适应而有效的图像凹点检测方法，并且较好地仿真了人类视觉系统；然后，结合信息传递和信息耦合特性，将PCNN扩展成PCNNs，提出了一种基于PCNNs的图像融合算法，能够将多个传感器获取的同一目标的图像信息融合到一幅图像中，有效模拟了人类视觉系统；另外，结合PCNN的同步脉冲发放特性、捕获特性和波的传播竞争特性，开拓地将PCNN用于模式分类中，提出了基于耦合神经元点火捕获／抑制特性的分类方法和改进的约束距离下的PCNN分类方法，前者可实现对样本空间中任意复杂分布训练样本的稳健非线性分类，而后者能够消除训练样本中刺点对分类的影响；最后，结合累积差分图像思想、PCNN波的形成与传播特性，通过各神经元之间连接取向来选择与控制自动波的流向，将PCNN用于运动视觉分析中的运动轨迹模拟及运动方向检测。

Result: Moderate to strong immunostainning was observed at the circularmuscle,submucosal region in normal bowel and in ganglionic bowel from patients with HD.

电镜下在环形肌近粘膜缘及环形肌外层见肠间质细胞与平滑肌细胞之间、平滑肌细胞与平滑肌细胞之间有缝隙连接存在，在无神经节细胞肠段未见此结构。

Every node of the chirp sonar connects with data conversion through TCP/IP. With one or more nodes added, the processing ability of the system can be geminated without changing the software.

该系统应用于海底石油管线探测与定位，系统中的每个处理节点与数据采集转换部分采用TCP/IP网络连接，可以通过物理上添加一个或多个处理节点成倍的提高系统的信号处理能力，而在软件上无须大的改动。

If the spermatic cord to connect the testis short channel peritoneal inguinal canal stenosis or prematurely closed, and connected at the bottom of the scrotum gubernaculum testis missing, testis and surrounding tissue with adhesion or formation of adhesions, scrotal hypoplasia, testicular gubernaculum is not along the direction of walking enter "岔道," and so the situation can be caused by cryptorchidism.

如连接睾丸的精索过短，腹膜信道腹股沟管狭窄或过早闭合，与阴囊底部相连的睾丸引带缺失，睾丸与周围组织粘连或形成粘连带，阴囊发育不良，睾丸未沿引带方向行走而进入&岔道&等等情况都可造成隐睾。

Histological examination demonstrated bone formed in accordance with intramembranous ossification in distraction zone and bony connection between the transport disc and mesial mandible on right side; On the left side, there was a big defect at the area of both distraction and defect zone because of dislocated bone transport disc, and no obvious new bone regeneration was found.

组织学检查：见9周后右侧牵张区形成新生骨质，符合膜内成骨，传送盘与近中牵张区形成骨性连接；左侧由于骨传松盘离开缺损区，牵张区与缺损区形成一较大的凹陷，未见明显新骨形成。

To the contrary, the supraorbital ridge and the forehead of the Maba Man are joined.

主要依据是马坝人的眉嵴与前额连接的地方没有爪哇猿人和中国猿人那样明显的深沟，而是与前额相连。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！