与...连接

Thereafter,DNA fragment of Hyl-1-Amp-Hyl-1 was subcloned into pBR322 vector,the resulting construct was then checked by PCR and restriction analysis for the proper configuration of the knockout vector pBR322-SA.

该载体与质粒pBR322分别用EcoRⅠ、PstⅠ双酶切后进行连接，得到基因敲除的重组载体pBR322SA，PCR 及限制性酶切分析，构建的敲除载体与设计结果相符。

Analyzing the characteristics of Bach's work, combining with application and general using law of pedal, the paper presents the reasons of debate and puts forward some suggestions: connecting two tunes, retouching psalterium tune and resonating chord need pedal when playing Bach's piano works; auditory is the key to decide whether to use pedal or not; using pedal should consider arrangement of harmony in Bach's polyphonic works.

依据巴赫作品的特点，结合踏板的使用与一般运用规律，弹奏巴赫的钢琴作品时，在连接两音、润色琴音及和弦共鸣时是需要使用踏板的，听觉是决定是否使用踏板的关键，使用踏板应特别考虑到巴赫复调作品中和声的安排与进行，即应做到"心耳相随，有机结合"。

In addition, PUL is interfaced real-time to ConneXion, PPD's proprietary computer system, to assure consistent management and reporting.

协和洛奇与PPD的计算机系统ConneXion实现实时连接，保证了管理与报告的一致性。

An upper base (1) of the reaction device is coaxially arranged into a water cooling sleeve (2); the connection of the upper base and the water cooling sleeve is welded and sealed to form a water cooling chamber; an anode current collecting Pt net (12) is used for collecting current from the anode of a button-typed solid oxide fuel battery (16); two ends of an anode current collecting Pt wire (15) are respectively used as an reference electrode and a testing electrode; the temperature measuring point of a temperature measuring thermal-couple (17) and the button-typed solid oxide fuel battery (16) keep in the same height; the bottom of an anode chamber pipe (6) is inserted into the upper hole of a lower base (43); the lower surface of a quartz sand sintering plate (42) is provided with a carbon fuel thermal-couple (56); all pipes and sealing heads (46) are hermetically connected with each other.

该反应装置上底座(1)同轴放入上水冷套(2)中并对连接处焊接密封形成水冷腔体；阳极集流铂网(12)用于从纽扣式固体氧化物燃料电池(16)阳极上收集电流，阳极集流铂丝(15)两头分别用作参比电极和测试电极；测温热电偶(17)测温点与纽扣固体氧化物燃料电池(16)保持同一高度；阳极腔体管(6)底部插入下底座(43)上部孔中；石英砂烧结板(42)下面布置碳燃料热电偶(56)；所有管件与封头(46)连接密封。

We redid the attack experiments based on Neighbor Discovery function on the established security link, the theories and steps are as same as above.

我们在建立的安全连接上重做基于邻居发现功能的攻击实验，原理与步骤与之前相同。

Alpha motor neurons that innervate muscle fibers are located in the ventral horns of the spinal cord, and comprise the efferent limb of this reflex arc.

这是一个单突触反射通路，运动依赖感受器的反馈，间接的Ⅰa传入纤维也在后角与抑制的中间神经元突触，这就能通过依次激活与α运动神经元连接的拮抗肌来抑制主动肌的紧张。

In the special case of GLM—normal linear model with classical link function,we compare our methods with existing ones and our methods perform more excellently.Finally,sequential detection of structural changes is studied in nonparametric regression models o Based on Nadaraya-Watson estimator of regression function,we calculate the residual series and construct "residual cumulative sum process" with weight values of kernel estimator of "long-run average density function".

在随机模拟一节中，给出了布朗桥的h-增量过程的"Max-Max"泛函统计量的经验分位数表，并在随机模拟中验证了文中所提"部分和"与"滑动和"方法的有效性，同时在广义线性模型取"典则连接函数"时的特例—正态线性模型场合，与已有方法进行了对比，结果显示文中所提方法表现更优。

When the manual position, the hand wheel and automatic enforcement agencies stem connection; when the automatic position, the SOLENOID REMOTE CONTROL FLOATING VALVE EN106X hand wheel and automatic decoupling the implementing agencies of the stem does not affect the self-executing agency action.

当手动位置时，手轮与自动执行机构的阀杆连接；当自动位置时，手轮与自动执行机构的阀杆脱钩，不影响自动执行机构的动作。

In the region upstream of vortex breakdown, the sectional streamlines emanating from the leading edge spiral inward to primary vortex and in the region downstream of the breakdown, on the other hand, the streamlines separating from the leading edge are not entrained into the vortex core in the instantaneous sense. Along the axis, the topology of the primary vortex alters several times starting from stable focus near the apex to unstable focus, and lasts back to stable focus near wake edge, the stable hmit cycle and unstable limit cycle are shown in this evolution.

发现在截面流线谱中，即使奇点的类型、数目及位置相同，奇点的连接方式也有可能不同；旋涡破裂点前，主涡由来自前缘的力线卷绕而成，主涡焦点与前缘半鞍点相连，旋涡破裂后，来自前缘的力线不再卷入主涡，主涡焦点与对称面上的鞍点相连：在流体由翼尖流向后缘的过程中，截面流线谱的拓扑结构发生了多次变化，主涡焦点相继由稳定状态转变为不稳定状态，最后又转化为稳定状态，并先后出现了稳定的极限环及不稳定极限环。

This circuit is more simple, convenient connectivity, the choice of its parameters with the speaker impedance has a direct relationship, and the speaker impedance is a function of frequency, with larger deviation from the nominal value, so the error is also large, is not conducive to adjust.

此电路较为简单，连接方便，它参数的选择与扬声器阻抗有直接的关系，而扬声器的阻抗又是频率的函数，与标称值偏离较大，因此误差也大，不利于调整。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！