与...连接

The company is located in the Yangtze River Delta, Jiaxing City in Zhejiang over the southeastern part of the new town, while the new town is a town with a long history of the Jiangnan, 12, from Jiaxing, with road, rail and water transportation to facilitate the transport network, township haiyantang Runs through the town, with the same Beijing-Hangzhou Grand Canal, Highway and Expressway connect Pottinger Jiasu exports Jiaxing Highway Department is located not far from the Company and convenient transportation.

本公司位于长江三角洲，浙江嘉兴市东南部的余新镇，余新镇是一个历史悠久的江南古镇，距嘉兴市区12里，具备公路、铁路与水运便利的交通网络，镇区海盐塘贯穿全镇，与京杭大运河相通，公路与沪杭甬高速相连接，乍嘉苏高速公路嘉兴出口处就位于本公司不远处，交通便利。

When the turn-table slewed, the hydraulic motor transmitted power output which enabled pinions on the output shaft to revolve.

进行回转时，液压马达输出动力，通过回转减速器减速后带动输出轴上的小齿轮旋转，小齿轮与回转支承的齿圈啮合，由于回转支承的齿圈与车架刚性连接，因而回转减速器带动与之相连的转台回转。

There are chiefly two models explaining the S4 movement: the helical screw (S4 move translationally and rotationally in a gating canal which is composed of part of the channel protein), and the paddle model (S4 lying at the channel periphery and moving as one unit with S3b).

本文即藉由S3与S4间之连接段(S3-4 linker)和S4本身之非碱性胺基酸的突变，来探讨S4在去极化时移动的方式与距离，以及包围在S4外侧之主要电阻区的环境与位置。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Inputs and Outputs Front Panel: Phones: Standard headphone output jack (gold-plated) Video 4: Gold-plated stereo RCA jacks, gold-plated RCA composite video jack and S-video jack Digital Optical 3 Input: Standard Toslink digital input with a plastic protective plug Digital Coax 3 Input: Standard gold-plated coaxial digital jack Back Panel: AM Loop Antenna Input: Two thumb screw connections FM 75-ohm Antenna input: Threaded "F" type connector Tape Input/Output: Stereo RCA jacks Video 3 Input: Stereo RCA jacks, RCA composite video jack and S-video jack Video 2 Input/Output: Stereo RCA jacks, RCA composite video jacks and S-video jacks Video 1 Input/Output: Stereo RCA jacks, RCA composite video jacks and S-video jacks DVD Input: Stereo RCA jacks, RCA composite video jack, and S-video jack CD Input: Stereo RCA jacks Monitor Output: RCA composite video jack and S-video jack Subwoofer Preamp Output: 1 RCA jack Digital Optical 1 Input: Standard Toslink digital input with a plastic protective plug Digital Optical 2 Input: Standard Toslink digital input with a plastic protective plug Digital Coax 1 Input: Standard coaxial digital jack Digital Coax 2 Input: Standard coaxial digital jack Digital Optical Output: Standard Toslink digital output with a plastic protective plug Digital Coax Output: Standard coaxial digital jack Remote Control In/Out Jacks: One mini-jack input and one mini-jack output Speaker Outputs: Binding post outputs for front left, front right, surround left, surround right, and center speakers (posts are not 5-way binding posts, because each post has a plastic collar that prevents used with spade lugs and the posts are too far apart to use dual banana plugs) AC Outlets: One unswitched AC outlet and one switched AC outlet

投入和产出接待小组：电话：标准耳机输出插孔视频4 ：镀金立体声RCA插孔，镀金的RCA复合视频插孔和S - Video插孔数字光学3输入：标准Toslink数字输入与一个塑料保护插头数字同轴3输入：标准镀金同轴数字接口背板：上午环形天线输入：两个拇指螺丝连接调频75欧姆天线输入：线程的& F &型连接器，磁带输入/输出：立体声RCA插孔视频3输入：立体声RCA插孔，插孔的RCA复合视频和S - Video插孔视频2输入/输出：立体声RCA插孔，莲花复合视频插孔和S - Video插孔视频1输入/输出：立体声RCA插孔，插孔的RCA复合视频和S端子接口的DVD输入：立体声RCA插孔，插孔的RCA复合视频和S -视频CD输入端插孔：立体声RCA插孔监视器输出：复合视频的RCA插孔和S - Video插孔低音前置输出： 1的RCA插孔数字光1输入：标准Toslink数字输入与一个塑料保护插头数字光2输入：标准Toslink数字输入与一个塑料保护插头数字同轴输入1 ：标准同轴数字接口数字同轴2输入：同轴数字接口标准数字光输出：标准Toslink数字输出塑料防护插件数字同轴输出：标准同轴数字接口远程控制在/输出插孔：一个迷你插孔输入和1个小型插孔输出扬声器输出：结合后产出的左前方，右前方，左环绕，右环绕和中心发言（职位不是5个具有约束力的方式，因为每个职位有一个塑料环，可以防止使用铁锹派和职位过于遥远使用双香蕉插头）交流插座：一个unswitched AC插座和一个开关AC电源插座

Study on west Namco thrust Three ductile shear zones were discovered in Gayalong, Elongnubu and Shenjue-Gechangchayu, and geological features, deformation, motion direction and structural pattern are studied in detail for major thrust faults, ductile shear zones and folds in west bank of Namco Lake, leading to identification of west Namco thrust . And west Namco thrust and ophiolite mélanges outcropped from west Namco Lake to Yongzhuzangbu coexist together, indicating another tectonic suture in the Tibetan Plateau termed as the Namco-Yongzhu suture or NYS with its east extension to Jiali fault.

纳木错逆冲推覆构造研究：发现纳木错西岸嘎龙垭韧性变形带、饿弄怒布舍糜棱岩带和生觉—各昌茶玉韧性剪切带，详细观测典型逆冲断层的地质特征、变形特点、运动方向和序次关系，分析逆冲断层、褶皱构造、韧性剪切带的几何配置关系和动力学成因联系；分析纳木错逆冲推覆构造的空间影响范围，向东延伸与嘉黎逆冲断裂相连接，向西延伸与果忙错—永珠藏布构造带相归并，与纳木错西岸蛇绿岩、永珠藏布—果忙错蛇绿岩共同组成拉萨地块中部最重要的区域构造，命名为纳木错—永珠缝合带。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

In reservoir design one sometimes meets with submerged structures, such as the underwater retaining wall, the foundation-rested immersed, plate which connects shore to intake-tower, the half-submerged navigation-guiding plate, a strut plate positioned midway in water depth, and also a diversion wall from shore.

在实际工程中，有时遇到某些建筑物是潜没在水中的，如水库水面以下的挡土墙和水库进水塔与岸边连接的撑墙；又有时遇到挡水建筑物与基础脱离，如水库中的导航板；另外还有一些既潜没在水面以下又与基础脱离的建筑，如水中撑板；还有岸边的平板。

The hybrid induction motor includes a housing; a stator installed in the housing and having a stator core portion and a stator coil portion; a rotor coupling body having a rotary shaft rotatably installed in at the housing, a cage rotor portion rotating integrally with the rotary shaft, and a permanent magnet rotor portion coupled to a circumference of the cage rotor portion with a certain air gap so as to be freely rotatable with respect to the rotary shaft; and a reverse rotation preventing switch, when the rotary shaft rotates reversibly, coming in contact with the permanent magnet rotor portion which is moved along the shaft by thrust generated by the rotor coupling body and cutting off power being supplied to the stator coil portion.

公开了一种混合式感应电机。所述混合式感应电机包括：壳体；安装在壳体中并且具有定子铁心部分和定子线圈部分的定子；篆字联接体，具有可旋转地安装在外壳中的旋转轴、整体地与旋转轴一起旋转的鼠笼式转子部分，以及永磁体转子部分，该部分以一定气隙连接到鼠笼式转子部分的周围以可相对于旋转轴自由旋转；以及防止反转开关，当旋转轴反转时，与永磁体转子部分形成并且切断提供给定子线圈部分的电源，其中当旋转轴反转时，转子联接体产生推力，通过该推力永磁体转子部分与反转防止开关相接触。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！