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The direct interaction of copper ion with CuZnSOD was studied by fluorescence spectroscopy, and a type of 1:1 stable complex has been generated, which was certified by the quenching experiment of intrinsic fluorescence. The process of the generated complex shown by dynamic analysis of fluorescence quenching, consisted of two steps, in which the first step was a quick association process of double molecules to generate a loose complex, and the second step was a slow process of single molecule, where the loose complex was isomerized into the compact complex.

通过荧光光谱方法研究了这种相互作用，内源荧光的猝灭实验表明Cu(上标 2+)与CuZnSOD形成1：1型稳定配合物；荧光猝灭的动力学分析表明配合物形成过程由两个独立步骤完成：第一步是双分子快速缔合过程，形成了结合疏松的配合物，第二步是单分子慢速过程，即松散的配合物&异构化&成为结合紧密的配合物。

The direct interaction of copper ion with CuZnSOD was studied by fluorescence spectroscopy, and a type of 1∶1 stable complex has been generated, which was certified by the quenching experiment of intrinsic fluorescence. The process of the generated complex shown by dynamic analysis of fluorescence quenching, consisted of two steps, in which the first step was a quick association process of double molecules to generate a loose complex, and the second step was a slow process of single molecule, where the loose complex was isomerized into the compact complex.

通过荧光光谱方法研究了这种相互作用，内源荧光的猝灭实验表明Cu2＋与CuZnSOD形成1∶1型稳定配合物；荧光猝灭的动力学分析表明配合物形成过程由两个独立步骤完成：第一步是双分子快速缔合过程，形成了结合疏松的配合物，第二步是单分子慢速过程，即松散的配合物&异构化&成为结合紧密的配合物。

The binding constants K and the number of binding sites n of KAE with DNA at 25, 32 and 39℃ were calculated to be 3.87×10^3Lmol^(-1) and 0.85, 6.71×l0^3Lmol^(-1) and 0.92, 9.73×10^3Lmol^(-1)and 0.92, respectively.

计算了25、32和39℃下，山柰酚与DNA相互作用的结合常数K和结合位点数n分别为3.87×10^3Lmol^(-1)、0.85，6.71×10^3Lmol^(-1)、0.92和9.73×10^3Lmol^(-1)、0.92。

Krana also physically interacts with ATM via its N-terminal KRAB domain. Thus, Krana bridges ATM to p53 and stabilize the interaction between p53 and ATM in vivo which is also abolished by heat shock or MMS treatment.

我们发现，Krana通过其N端的KRAB结构域与ATM结合，作为桥梁蛋白稳定ATM和p53在细胞内的结合；热休克和MMS处理后，三者的相互作用解离。

The forming process for the high-strength, steel-base modified polyformaldehyde composite material in five-laver structure is to melt modified polyformaldehyde by heating and roll to make the melt polyformaldehyde permeate into the gaps gaps between the large-grained spherical copper powder and screw copper nails.

四层结构的钢基铜塑复合材料的成型过程，是将改性聚甲醛加热熔化，经轧制，使熔融的聚甲醛渗透到大颗粒球形铜粉的间隙中，解决了三层材料复合过程中，熔融的聚甲醛水平流动性强过垂直方向的渗透性，造成了结合强度低的缺陷，使聚甲醛层与铜粉及基体结合更牢固，提高了其承载和抗剪切的能力。

The forming process for the high-strength, steel-base modified polyformaldehyde composite material in five-layer structure is to melt modified polyformaldehyde by heating and roll to make the melt polyformaldehyde permeate into the gaps between the large-grained spherical copper powder and screw copper nails.

五层结构的高强度钢基改性聚甲醛复合材料的成型过程，是将改性聚甲醛加热熔化，经轧制，使熔融的聚甲醛渗透到大颗粒球形铜粉的间隙中，解决了三层材料复合过程中，熔融的聚甲醛水平流动性远强过垂直方向的渗透性，造成了结合强度低的缺陷，使聚甲醛层与铜粉、螺旋铜钉及基体结合更牢固，提高了其承载和抗剪切的能力。

Lectin can be used to analyze the structures of sugarchains.

凝集素(1ectin)是一类糖结合蛋白，能专一地识别某一特定结构的单糖或寡糖中特定的糖基序列而与之结合。

Therefore, we used a computer-based high throughput screening method to screen drug library to identify the possible binding drugs, thus increasing the added value for nsLTPs in drug delivery systems.

我们利用了电脑助药物筛选的技术来进行筛选，试找出可能与此一蛋白结合的药物，并结合萤光光谱的方法加以验证。如此，可进一步提高此一蛋白质的附加价值。

On the basis of development of literates since 20th century, this paper divides it to three time quantum: the modern literature 30year, previous 30 years after the founding of the nation and the new time.

对20世纪以来小说中苦难叙事的流变做了简单的历史性介绍，结合文学发展的历史与特点，将二十世纪文学分为现代文学三十年、建国后前三十年和新时期三大时间段，在相应的时间段中结合代表性的作品来分析说明苦难叙事的发展流变。

This thesis deals with oil-water flow rate measurement using a venturi and develops a differential pressure-based two-phase flow rate measurement model, which does a terrific job with the use of a water fraction device. Meanwhile, Coriolis flow rate meter is also studied in the oil-water two-phase flow measurement and a SVM-based single-phase flow rate prediction method is proposed, realizing the prediction of single-phase flow rates. To overcome the disadvantages of both venturi and Coriolis flow meters, a double parameter measurement method is put forward, which combines the two meters and solves the problem of oil-water flow rate measurement. Last but not least, on the basis of the study of two-phase flow, a device for the separation of gas and liquid is designed and realizes the flow rate measurement of three-phase flow as oil, gas and water.

本文对文丘里管测量油水两相流流量进行了研究，建立了基于差压法的两相流流量测量模型，如果与含水率计相结合，可以较好的实现油水两相流的流量测量；同时研究了Coriolis流量计在油水两相流流量测量中的应用，提出了基于SVM的油水两相流分相流量预测方法，实现了油水分相质量流量的预测；针对文丘里管和Coriolis测量油水两相流存在的问题，提出了文丘里管和Coriolis流量计相结合的双参数测量方法，并实现了油水两相流的流量测量；最后，在对油水两相流研究的基础上，设计完成了一种气液分离装置，实现了油气水三相流的流量测量。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。