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This article unified the customer personalization demand the characteristic, the existence question as well as the production and the dispatch production management system has done the preliminary research, the union case analysis based on the customer personalization demand production and the dispatch to the economic development function, narrated and commented the existing some production scheduling method, proposed that the utilization blending inheritance algorithm solution based on the customer personalization demand's production scheduling question's methodology, has simultaneously established take the customer demand as the guidance production scheduling architecture.

本文结合顾客个性化需求的特点、存在的问题以及生产与调度生产管理系统做了初步的研究，结合案例分析基于顾客个性化需求的生产与调度对经济发展的作用，评述已有的一些生产调度方法，提出运用混合遗传算法解决基于顾客个性化需求的生产调度问题的方法论，同时建立了以顾客需求为导向的生产调度体系结构。

We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

It creats a mutual space for human and plants by the integration of environment protection and energy saving, which is the goal seek by humans and reach the final art bourn by human and natural integration.

它创造了人与植物共生的空间，充分结合环境保护、绿色节能这一人类共同追求的目标，达到人与自然完美结合的艺术境界。

The hydrophobic value of Fuchs\' model of humic acidsand Alvarez\'s model of fulvic acids which combined with metal icon smaller than the other molecularmodels; but the refractiveness, polarity, approximate surface, grid surface, volume, mass of the Felbeck\'smodel k of the humic acids and Buffle\'s model of the fulvic acids which interacted with the metal iconsmaller than the other molecular models.

综合比较结果表明：Fuchs—HA和Alvarez—FA与金属离子结合物的疏水性小于其他它腐殖酸分子；Felbeck-HA和Buffle—FA与金属离子结合物的折射性、极性、近似表面积、栅格表面积、体积、质量同比小于其它腐殖酸分子。

Related with the traffic status of our country, the content of this paper is combined with practical application. The paper studies how to investigate traffic status in urban signal intersection, By field observing and videography, this paper get the geometry formal、traffic control and traffic running state of Xingtan road and Wenhuiyuan north road intersection, which can be support database for model building and model testing. On currently basic design principles of signal phase, through deep study of signal phase and canalization design.

本文结合我国目前的交通状况，着力将所要研究的内容与实际应用结合起来，研究了城市信号交叉口交通调查的方法，通过实地观测和摄像的手段获得杏坛路与文慧园北路交叉路口的几何形式、交通管理和交通运行状况的信息，以此作为建立模型和验证模型的支持数据，并在现有信号相位设计基本原则的基础上，深入研究了混合通行情况下的相位方案及渠化设计。

The RV antibody captured by the antibody-gold conjugate would migrate along the cellulose nitrate film and bind specifically with the RV glycoprotein to form a red line visible to the naked eye.

样品中狂犬病毒抗体与金标记抗体结合后沿硝酸纤维素膜移动，与膜上的糖蛋白特异结合形成肉眼可见的红色线条。

Based the analysis of the modeling characteristic of different chairs,main modeling factors of chair-back throughout the design process were summarized,such as the people-oriented design concepts,the combination of contemporary design concepts and design elements,integration of materials and modeling language,to provide references for the related designers.

通过椅背造型特点的比较分析，总结了椅背造型设计过程中主要的设计切入点，如在作品中要充分融入以人为本的设计理念，结合当代的设计理念与设计元素，将材料与造型语言有机结合。

The combination of cold and heat storage with heat pumps can be combined with water/humidity regulation.

的调节相结合的冷和热储热与泵结合起来，可以与水/湿度。

Methods Mimicry and speculation on possible displaced amino acids in proper positions of peptide increased the biological effects of TNFR blocking peptide by compote modeling. The original peptide and its mutant were synthesized. The competitively inhibitory effects on green fluorescence protein-tumor necrosis factor fusion protein with TNFR were compared between original peptide and its mutant (receptor competitively inhibitory binding assays). The inhibitory effects on cytotoxicity were compared between original peptide and its mutant.

通过计算机模建，模拟并推测提高TNFR封闭肽生物学效应需置换的位点和氨基酸；合成原封闭肽和突变肽；受体竞争抑制结合实验比较原肽与突变肽对绿色荧光蛋白－肿瘤坏死因子融合蛋白结合TNFR的竞争抑制效应；细胞毒作用抑制实验比较原肽与突变肽对TNFR的封闭效应。

PDM is the abbreviation of Product Data Management. It is a valid method that relies on the IT technology to realize the optimization of enterprise management, which comes from the combination of the scientific management framework and the realistic enterprise problem, the computer technology and the enterprise culture.

PDM是Product Data Management的缩写，它是依托IT技术实现企业最优化管理的有效方法，是科学的管理框架与企业现实问题相结合的产物，是计算机技术与企业文化相结合的一种产品。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。