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In the methods of research, this paper pays attention to the combination of demology, economics and sociology etc, uses the scientific elements in the existing research results, and unites it with the reality of China closely. In the background of dualistic structure, it emphasizes particularly on the rural old-age security issues to do an intersectional research. It combines the theory with the reality, combines the qualitative research and quantitative research. The countermeasures are realistic, feasible and far-sighted.
在研究方法上,本论文注重人口学、经济学、社会学等理论方法的结合,合理借鉴国内外已有研究成果科学成份,紧密结合中国实际,在"二元结构"大背景下,侧重对农村养老保障问题进行交叉研究,做到理论与实际想结合,定性与定量分析相结合,对策研究注重现实性、可行性和前瞻性。
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ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.
ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子,在许多同种的真核生物有它的同源基因,这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关,而这个家族的蛋白质和DNA的结合能,各种ARID蛋白质的专一性尽相同,过大致上偏好於和AT-rich的序结合;我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因,分别是garid1和garid2,我们首先对於garid1做分析;将AU1标记接到gARID1转染形鞭毛虫,用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下,过其阳性染色和蛋白质表现有明显增加;EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合,经由突变序分析,也显示gARID1的结合序为AGATC和AATAAAATA,随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。
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In this process, educational, independent, progressive, and systematic principles should be followed, with general education and personal guidance combined, comprehensive education and process instruction combined, conventional education and particular education combined, campus education and extramural education combined, to effectively implement career development education via curriculum teaching, theme activity, social practice and so on.
应该遵循教育性、主体性、递进性、系统性等原则,采取一般教育和个别指导相结合、全面教育和过程指导相结合、常规教育与专门指导相结合、校内教育与校外教育相结合等方法,通过课程教学、主题活动、社会实践等途径,有效实施大学生职业发展教育。
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Firstly, the paper, combining the characteristic of synchronous pulse bursts and inhibition with the modified PCNN model, presents a way of finding the foveation points in the images adaptively and effectively, and simulates the human vision system. Secondly, PCNN is extended to PCNNs, based on the properties of information couple and transmission, an algorithm that is used to fuse images of the same target got by several sensors to an image is presented to simulate the human vision system. Thirdly, combining the properties of synchronous pulse bursts, capture, and transmission and competition of waves, the paper presents two ways of classification, one is an algorithm based on the properties of neuron to capture and inhibit to classify the data taking on any complex unlinear distribution robustly, the other is based on the restricted distance and modified of the former to remove the influence of inferior samples in classification; Finally, based on the accumulative difference pictures, and the forming and transmission of PCNN wave, selecting and controlling the direction of autowave by connecting the neighbouring neurons selectively, the paper presents a way to simulate the tracks of moving object and detect the moving direction.
首先结合PCNN的同步脉冲发放和侧抑制特性,提出了基于改进型PCNN的图像凹点检测算法,该算法是一种自适应而有效的图像凹点检测方法,并且较好地仿真了人类视觉系统;然后,结合信息传递和信息耦合特性,将PCNN扩展成PCNNs,提出了一种基于PCNNs的图像融合算法,能够将多个传感器获取的同一目标的图像信息融合到一幅图像中,有效模拟了人类视觉系统;另外,结合PCNN的同步脉冲发放特性、捕获特性和波的传播竞争特性,开拓地将PCNN用于模式分类中,提出了基于耦合神经元点火捕获/抑制特性的分类方法和改进的约束距离下的PCNN分类方法,前者可实现对样本空间中任意复杂分布训练样本的稳健非线性分类,而后者能够消除训练样本中刺点对分类的影响;最后,结合累积差分图像思想、PCNN波的形成与传播特性,通过各神经元之间连接取向来选择与控制自动波的流向,将PCNN用于运动视觉分析中的运动轨迹模拟及运动方向检测。
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The results indicated that the extraction rate of mineral from pollen in 70℃ water was the highest. the extraction rates of four mineral: Fe, Zn, Cu and Mn were 6. 931%, 52. 786%, 44. 443% and 46. 549% respectively. Second speciation presented in the form of exchangeable and nonexchangeable speciations, organic and inorganic speciations at the same time. The contents of both Fe and Mn in nonexchangeable speciation were more than 50%, most contents of Fe、 Zn、Cu、Mn were inorganic speciation. Among the four elements, the content of protein binding speciation of Fe was the highest: 47. 305%. The average mineral contents of soluble carbohydrate and grease binding speciation were less than protein binding speciation.
结果表明:70℃水浸泡花粉,矿物质浸出率最高,Fe、Zn、Cu、Mn四种元素总提取率分别为6.93l%、52.786%、44.443%和46.549%;次级形态以稳定态与不稳定态、有机态与无机态等多种形态共存,其中Fe、Mn元素不稳定态含量均大于50%,Cu、Zn、Mn、Fe元素大部分以无机态形式存在;四元素中Fe元素蛋白质结合态最高,达47.305%,可溶性糖类结合态及脂肪结合态平均含量比蛋白质结合态平均含量少。
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The antibody is bound to an inert polymer support ,then exposed to reacts with the antigen at a different epitope is added. The second antibody used is one that has an enzyme attached to it that coverts a colorless or nonfluorescent substrate into a colored or fluorescent product. The amount of second antibody bound, and hence the amount of protein antigen present in the original sample, is determined by quantification of the intensity of color or fluorescence produced.
将抗体结合在一惰性聚合物载体上,然后与样品接触,洗脱未经结合的蛋白质加入与抗原其他表位也可以结合的另一种抗体,在所用的第二抗体上结合有酶,此酶可把无色的或无荧光的底物转化有色或显示荧光的产物。
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The compensation system of first-line workers of state-own coal enterprise was redesigned under the direction of compens
结合相关薪酬理论,对国有煤炭企业一线员工的薪酬体系进行了全新设计,构建了由基本薪酬、效率薪酬、安全薪酬及福利薪酬四大部分组成的以激励为导向的&绩效薪酬体系&,详细论述了各大组成部分的设计方法和设计步骤:综合考虑企业生存状况和社会收入状况,采用以劳动定额和社会平均收入相互平衡的方法,设计出一线员工的基本薪酬模块;提出了用专家评议法确定施工难度等级指标体系的相关权重、用综合模糊评价法来确定作业难度等级、把团队人力资源要素与作业区域的施工难度状况紧密结合起来测算团队业绩,构建出体现团队业绩和贡献的效率薪酬模块;构建了以&安全抵押&制度为核心,以月度安全收入、季度安全收入、年度安全收入为框架,形成了短期、中期与长期安全收入相结合的安全薪酬模块;结合国有煤炭企业一线员工来源复杂、身份复杂、需求不同的现状,采用了员工参与性和自决性极强的&自助式&福利薪酬模式。
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As a result, this seriously obstructs economic development step of our country. Therefore, starting from system and no-system, the paper sets up incentive and constraint mechanism according to the combination of material incentive and spirit incentive, long-term benefits and short-term benefits, system together with organize constraint and constraint by himself/herself, in order to solve the problem of agency and individual objective of manager deviating enterprise objective.
因此本文从制度和非制度建设出发,按照物质激励与精神激励相结合,长期利益和短期利益相结合,制度和组织约束与自我约束相结合,构建了对经营者的激励与约束机制,以期解决企业中存在的代理人问题和经营者的个人目标与企业目标相脱离的问题。
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These studies demonstrate that the more abundant D form interacts with spermatozoa transiently, possibly with a specific receptor on the sperm surface, consistent with a capacitation-suppressing function during sperm transit and storage in the epididymis, and also confirm a tightly bound population of the E form that could act in the female reproductive tract.
研究证明在附睾中运输和储藏期间,大量的D型CRISP-1与精子瞬时的结合与精子的抑制获能功能相关。D型CRISP-1可能与精子表面的受体相结合。同时也证明了E型CRISP-1与精子的紧密地结合模式可能在雌性生殖道中发挥作用
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These studies demonstrate that the more abundant D form interacts with spermatozoa transiently, possibly with a specific receptor on the sperm surface, consistent with a capacitation-suppressing function during sperm transit and storage in the epididymis, and also confirm a tightly bound population of the E form that could act in the female reproductive tract.
研究证明在附睾中运输和储藏期间,大量的D型CRISP-1与精子瞬时的结合与精子的抑制获能功能相关。D型CRISP-1可能与精子表面的受体相结合。同时也证明了E型CRISP-1与精子的紧密地结合模式可能在雌性生殖道中发挥作用→如果您认为本词条还有待完善,请编辑词条
- 推荐网络例句
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This one mode pays close attention to network credence foundation of the businessman very much.
这一模式非常关注商人的网络信用基础。
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Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.
扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。
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There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.
双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。