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Based on the detailed analysis of a variety of factors affecting stability of taxol production in Taxus cells, it was discovered that initial cell density and the relevant concentrations of substrates, elicitors and precursors significantly affected stability of taxol production; farther study showed that addition of elicitors at appropriate cellular state was the key to steadily improve taxol yield, especially, the proper cellular state was at the end of lag phase and at the beginning of the stationary phase, which was also proved by the cellular ultrastructure.

对影响红豆杉细胞紫杉醇产量稳定性的各类因素进行详细分析,发现细胞的接种密度以及与之相适应的培养基的基质浓度、诱导子浓度和前体浓度是影响紫杉醇产量稳定的重要因素;进一步研究发现,选择细胞处于合适的状态时加入诱导子是稳定提高紫杉醇产量的关键,细胞合适状态特别是延迟期末和稳定期初最合适,细胞状态的超微结构观察结果也证明了这一点;同时研究发现采用合适的同步化处理获得的细胞初始周期时相加入诱导子与紫杉醇产量稳定密切相关,且以低温同步化处理得到的细胞周期的分裂中期相的细胞加入诱导子不仅大大提高了紫杉醇的产量,而且诱导子加入时间大大缩短,有利于大大提高紫杉醇生产率。

This invention provides a method for transferring third party landing based on IP network including: a door network station carries a ciphered KEY generated randomly by a service server to send a link request to a third party network station so as to avoid transmission of user ciphered codes and ensure absolute security of user codes, besides, since the third party network station calls back the Web server by copying the ciphered KEY to verify the cryptographic key so as to secure the absolute safety of shaking hands, since the door network station does not transfer user ciphered codes with the third party network station, which is not necessary to synchronize with the door station about codes, thus the requirement of complicated maintenance to synchronization on ciphered codes to the third party is reduced.

本发明提供了一种基于IP网络转接第三方登陆的方法,其关键是,由门户网站携带业务服务器随机生成的加密KEY向第三方网站发送链接请求,避免了用户密码的传输,因而保证了用户密码的绝对安全性。再有,由于第三方网站通过复制加密KEY回调Web服务器,由Web服务器进行密钥验证,因而又保证了握手的绝对安全性。应用本发明,由于门户网站与第三方网站之间不传递用户密码,使得第三方网站无需与门户网站进行密码同步等机制的处理,在大大提高安全性的同时,降低了对第三方网站对于密码同步机制等复杂维护的要求。

At the same time, the importance of donor atoms pertaining to the side chain in the complexation reaction of three lariat azacrown ethers with alkali metal ions has been analyzed through the Fukui function.

同时,利用福井函数,对侧臂在氮基臂式冠醚与碱金属配位过程中的重要性进行了分析,结果表明氮基臂式冠醚的侧臂上供电子原子对整个冠醚分子与金属配位过程中有着十分重要的作用。

The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段,应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段;在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法;检测方法的特异性试验结果表明,该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应;检测方法的敏感性试验结果表明,通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg;对20份临床样品检测中,有10份为AIV阳性,其中有5份为H5亚型病毒阳性。

Single PCR reaction with two pairs of PCR primers designed on the markers respectively tightly linked with Cf-9 and Tm-1 genes in tomato, which be resistant to leaf mold and tomato mosaic virus. For the genes the PCR products were almost completely correspond to the amplified bands produced by single PCR primer.

利用同一PCR反应体系,对分别与番茄抗叶霉病的Cf-9基因和抗番茄烟草花叶病毒病的Tm-1基因紧密连锁的PCR标记进行了同时扩增筛选,扩增的特异性片段与单引物扩增片段吻合。

This book reviews the many areas of numerical analysis, including the configuration polynomial, finite difference, factorial polynomials, summation, Newton formula, operator and configuration polynomial, Cheung section, close polynomials, TaylM more item type, interpolation, numerical differentiation, numerical integration, and with the series, differential equations, differential equations, least squares polynomial approximation, minimax polynomial approximation, rational function approximation, triangular approximation, non-linear algebra, linear equations, linear programming, boundary value problems, MonteCarIo methods and so on.

本书综述了数值分析领域的诸多内容,包括配置多项式、有限差分、阶乘多项式、求和法、Newton公式、算子与配置多项式、祥条、密切多项式、TaylM多项式、插值、数值微分、数值积分、和与级数、差分方程、微分方程、最小二乘多项式逼近、极小化极大多项式逼近、有理函数逼近、三角逼近、非线性代数、线性方程组、线性规划、边值问题、MonteCarIo方法等内容。本书的特色主要表现在利用例题及大量详细的题解来透彻地阐明所述内容的内涵,同时附有大量的补充题以便读者进一步巩固和深化从书中获得的数值分析知识。

Based on specific requirement for the top-coal caving mining to the four-bar linkage ,a design method of the four-bar linkage is put forward in the paper in which the least difference function between the lemniscate locus and the power function curve is used as the main objective function.

基于综放工艺对支架四连杆机构的特殊要求,首次提出以双纽线与幂函数曲线之差值最小作为主要目标函数的数学模型,并在此基础上应用VisualBasic5 0编制了四连杆机构的优化设计程序,可以同时实现优化参数显示与优化结果的动态显

are made that the modern Chinese entertainment lexis is affected by society, culture and psychology. At the same time, it also has constructional function to them.

本文认为,现代汉语娱乐词汇一方面受到社会、文化与大众心理的影响,同时又对社会、文化与大众心理具有建构功能。

The molecular charge distribution of the reactivator is affect the reactivating efficacy directly. Moreover its association with hydroximic acid dissociation constant pAa closely, and it will have a great impact on reactivating ability indirectly.(3) There is likely to exist front molecular interaction between Cholinesterase Reactivators and OPC-inhibited AChE, the action sites mainly lie on the aromatic ring, the substitute R and the hydroixic acid group. They act on the aromatic residues of AChE by Π-Π conjugation .(4) The reached results by Comparative Molecular Field Analysis is useful to design new higher efficacy derivatives for 1, 2, 4-oxadiazole class nonquaternary Cholinesterase Reactivators.

2复能剂分子的电荷分布直接影响复活活性,同时对肟酸解离常数pKa具有重要影响,间接影响复活能力;(3)复能剂与磷酰化酶可能存在前线轨道作用,作用部位可能主要在芳香环及其取代基R和肟基上,与AchE的芳香族残基发生л-л电子作用;(4)通过比较分子力场法研究可以指导1,2,4-oxadiazole类非季铵肟胆碱酯酶重活化剂新化合物的合成,可能得到活性更高的复能剂。

We will work with line manager and HR staff to provide suitable talents for their long-term development needing.

我们不但能为客户提供全方位优质的招聘服务,同时还可以站在战略伙伴的高度与客户合作,提供更多的行业信息,从而为客户企业的内部组织结构建立、调整等工作提供咨询与帮助。

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推荐网络例句

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。