与...同时

The experimental study on energy dissipation for Yuanmanguan Power Station was carried out by the hydraulic model test, put forward a new energy dissipator style and its designing method on five crest spillway flood for arch dam.

利用水工水力学模型试验研究方法对圆满贯工程泄洪消能问题进行研究分析同时，为百米级拱坝在五表孔布置方面，提供了一种全新的泄洪消能工体型与设计思路，即采用宽尾墩窄缝纵向水舌与连续鼻坎横向扩散的布置形式，可以使水舌在纵向与横向实现全范围的扩散，消能率提高，适应不同运行方式需要的同时，缩短了RCC拱坝施工工期，节约了工程总投资。

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

That is an even more personal manner in this research. With attitude of the"flaneur" to go to the interpretation of the city's cultural observation.The scale of the picture book from huge scope to small, gradually in-depth the life of Taipei City experience. It displayed the "megacromic and microcosmic things" in this two scales of picture book. Finally, I arranged all images and let it become a personal thinking for interpreting of the experiences the life of Taipei.

本论文试图透过自身熟悉的沟通语言─绘本，作为个人对於台北城生活经验的认知书写，以自我个人化的第一人称描述我所见、所感觉到的台北城，同时以「步行的漫游者」作为空间实践的态度，并透过尺度上由大变小的差异角度呈现不同的绘本观点，这样的绘本过程，对於自身是一种深入台北城生活的方式，同时「漫游者」的态度更可以反应一种文化观察的眼光与反思，呈现出两种差异尺度的绘本─巨观与微观，最后将这些图像予以安排成一个观看城市的方法与态度。

In this paper, three methods for color image retrieval have been presented: hypercomplex SVD image retrieval, combination of hypercomplex convolution filter and hypercomplex SVD image retrieval and hypercomplex matrix correlation image retrieval. We made comparison among these hypercomplex-methods, and compared them with the traditional image retrieval methods. Experiment results showed that the hypercomplex-methods we presented in this paper could well conquer the problems existed in traditional image retrieval methods, and their retrieval results are much better. Meanwhile, this paper presented a new color image quality assessment based on hypercomplex SVD. It is a novel color image quality assessment that can be used as a graphical or a scalar measure to predict the distortion introduced by a wide range of noise sources. It reliably measures the distortion not only within a distortion type at different distortion levels, but also across different distortion types.

提出了超复数奇异值分解检索法，超复数卷积滤波与超复数奇异值分解相结合的检索法以及超复数矩阵相关检索法等三种超复数彩色图像检索方法，在对提出的三种算法进行比较的同时也将其与传统图像检索方法进行了比较，结果证明，超复数彩色图像检索法能较好地克服传统图像检索方法存在的问题，且检索结果更优；同时，本文提出了基于超复数奇异值分解的彩色图像质量客观评估法，这种全新的图形化与数值化相结合的方法不仅能够准确评估出彩色图像的不同失真等级，还能够区分不同的失真类型，从真正意义上实现了彩色图像的质量评估。

Flashing yellow, peaceful and elegent blue, vigous green……among 09SS collection, H.L especially select white, blue and yellow as the major colors. Besides, it can be seen the lastest international fashion trend from its simple design. With regard to the fabric selection, natural fabrics such as cotton, linen and silk etc. are adopted to embody natural, simple and free style and to express fashion concept. The fabulous matching between fabrics and cutting is used to meet needs for comfortable and casual clothing. Meanwhile, using bright and vivid color can still reflect jauntiness and elegence nature even if the products are finished.

热力四射的黄、宁静优雅的蓝、生机盎然的绿……今年的春夏新款，H.L特别选用以白色、蓝色及黄色为主要流行色，在设计简单的同时又融入国际最新流行风尚，以此体现女性的雅致与灵动。H.L在面料的选用上，以最能体现自然、简约、自由这一风格的天然质感的面料如棉、麻、蚕丝等，从面料中体现服装的内涵，追求面料与剪裁的高度和谐，以迎合都市人舒适、随意的着装需求；同时运用明快鲜艳的色彩，成品后依然保持本真的色调，彰显出活泼与高雅的气质。

There are three fundamental stratagems:(1) construct new economical ecol-ogy structure----to disconnect input of environment resources and economy de-velopment;(2) establish precautious landscape policies----there are three relatedand coinstantaneous processes: aggravation of landscape damage, knowledgeacquiring and public awareness.

同时，本文建设性地提出了景观恢复的3个基本方略：(1)确立新的经济的生态结构——使环境景观的投入与经济增长脱钩，通过4大投入要素与GDP关系的举例，分析讨论了生态结构与经济结构改变的趋势；(2)制定预防性景观、环境政策——通过损害环境、景观的代价举例分析，讨论了环境政策制定有三个相互关联而且同时发生的过程：环境景观损害日益加重、加剧；人类获取技术知识；公众觉悟。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

Results The activation of NF-κB, proliferation response, and expression of IL-4 and I L-5 mRNA and protein in T lymphocytes stimulated by PMA were significantly hig her than those of their blank control (P.01), while those indexes of T l ymphocytes stimulated by PMA and PDTC simultaneously were significantly lower th an those stimulated by PMA alone (P.01). The apoptotic index of T lympho cytes stimulated with PMA were significantly lower than that of their blank cont rol (P.01), and the apoptotic index of asthmatic guinea pig T lymphocytes stimulated with PMA and PDTC simultaneously were significantly higher than that stimulated by PMA alone (P.01). The significant positive correlations w ere found between the activation of NF-κB and the proliferation (r=0.64, P.001), and the expression of IL-4 and IL-5 mRNA and protein of T lymph ocytes, respectively (r=0.55-0.68, P.001). There was also signific ant negative correlation between the activation of NF-κB and apoptosis of T l ymphocytes (r=0.62, P.001). Conclusions NF-κB may participate in the signal conduction of PKC regulated proliferation, apoptosis and expression of IL-4 and IL-5 of T lymphocytes in asthma.

结果加入PMA培养的哮喘组T淋巴细胞NF-κB的活化、细胞增殖反应、IL-4和IL-5的mRNA和蛋白质的表达均显著高于其空白对照(P.01)，而同时加入PMA和PDTC培养的哮喘组T 淋巴细胞以上指标均显著低于只加入PMA培养的哮喘组T淋巴细胞(P.01)；加入PMA 培养的哮喘组T淋巴细胞的凋亡指数显著低于其空白对照(P.01)，而同时加入PMA 和PDTC培养的哮喘组T淋巴细胞凋亡指数显著高于只加入PMA培养的哮喘组T淋巴细胞(P 。01)。T淋巴细胞NF-κB的活化与增殖反应呈显著正相关(r=0.64，P.00 1)，与IL-4和IL-5的mRNA和蛋白质的表达也均呈显著正相关(r=0.55-0.68，P 。001)，而与凋亡指数呈显著负相关(r=-0.62，P.001)。

Experimental results indicated that the contaminants generally existed in mussel, such as α-HCH,β-HCH,γ-HCH,δ-HCH, o·p-DDT, p·p-DDT, p·p-DDD, were degraded completely under the above conditions, whereas DDE, aldrin and dieldrin were partly degraded. No detectable degradation of PCB 1242 and PCB 1248 were observed. Thus the conventional preparation of the contaminants using the columns packed with Florisil or SiO〓 could be left out.

测定贻贝中的PCBs时，以KOH甲醇溶液处理试样，在微波能作用下，试样的微波皂化与有机氯农药的微波碱解同时进行；测定土壤和沉积物中的PCBs时，以KOH甲醇溶液处理试样，在微波能作用下，PCBs的微波萃取与有机氯农药的微波碱解同时进行，1M KOH甲醇溶液对PCBs的微波萃取能力与纯甲醇溶剂相当。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。