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Methods TIL in the patients with PHC were separated with combinative enzymatic digestion of type Ⅰ,Ⅳ collagenases,type Ⅴ hyaluronidase and type Ⅰ deoxyribonuclease followed by discontinuous density gradients.Cytotoxicity of TIL to liver cancer cells was detected with MTT method.

采用Ⅰ型和Ⅳ型胶原酶、Ⅴ型透明质酸酶和Ⅰ型DNA酶四联酶消化,应用不连续密度梯度离心分离TIL,采用MTT比色法检测TIL对自体癌细胞及肝癌细胞株的细胞毒。

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The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作:对伸臂位于顶部的单层框架—筒体模型进行分析,提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。

Its advantage is that it can be used in smaller units.

其优点在于可以在较小的单位中应用。