不起作用的

Government will not be efficient unless the people as a whole accept leadership loyally and enthusiastically.

除非全体人民忠诚而又热烈地拥护政府的领导，否则政府是不起任何作用的。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

The resistance and long distance pressure meter is suit to measure the liquid ,steam. or gas which can't corrupt the cooper and steel.

电阻远传压力表适用于测量对钢及铜合金不起腐蚀作用的液体，蒸汽和气体等介质的压力。

Rontgen could not see anything coming out of the tube, but then he discovered that if he put the screen in the next room on the other side of a closed door, the rays still seemed to affect it.

栾琴看不出任何东西从管子里出来，但他发现假如他把银幕放在隔壁房间关起来的门的后面，射线似乎还是能对银幕起作用。

Weather is a product of many pLysical factors operating in and on the tropo here with the principal ones being the unequal heating of the earth's surface by the sun and the rather rapid rota tion of the planet on its axis.

天气是许多自然因素在对流层中起作用并对其施加影响的结果，其中太阳对地球表面加热不均等和地球自转比较快则是主要的因素。

The CAN-SPAM Act of 2003 in the United States is an example of a relatively useless, toothless law.

就像2003年美国通过的《CAN-SPAM法案》，这项法案就是一项不起实际作用的法律。

Your tools don't have to be top of the line, but they do have to work for you.

你的工具不一定是性能最好的，但只要对你起作用就好。

The occurrence of ore-body was controlled by faults and unconformable face-detachment zone.

对流体大规模运移而产生巨量的矿化富集，起主导作用的是断裂与不整合面。

This, however, means nothing. Snakes did not acquire their poison for use against man but for use against prey such as rats and mice, and the effects on these of viperine poison is almost immediate.

但是，这没有什么关系，因为蛇有毒液不是用来对付人的，而是对付它的猎物，诸如鼠类，毒液对这些猎物会立刻起作用。

This, however, means nothing. Snakes did not acquire their poison for use againstman but for use against prey such as rats and mice, and the effects on these of viperine poison is almost immediate.

但是，这没有什么关系，因为蛇有毒液不是用来对付人的，而是对付它的猎物，诸如鼠类，毒液对这些猎物会立刻起作用。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。