不败

Yet, oleander bloomed silently inside the gate. One bud withered, and another came out ;a tuft became yellow, and another blossomed out. You could not see the most prosperous period or the most withered time either in the vernal spring wind, or in the summer thunderstorm, or in the deep lonely autumn. It greeted wind and displayed her beauty every day, from spring to autumn, from the bloom of winter jasmine flower to the decay of the plantain lily and chrysanthemum.

然而，在一墙之隔的大门内，夹竹桃却在那里悄悄地一声不响，一朵花败了，又开出一朵，一嘟噜花黄了，又长出一嘟噜；在和煦的春风里，在盛夏的暴雨里，在深秋的清冷里，看不出什么特别茂盛的时候，也看不出什么特别衰败的时候，无日不迎风弄姿，从春天一直到秋天，从迎春花一直到玉簪花和菊花，无不奉陪。

Whereas,beside the entrance door the oleander quietly continue its circles,while one fall after another open,and one wither till another born.Enjoying in such a pleasantly spring breeze,the rainstorm in midsummer as well as the chilly in late autumn,from which I could not distinguish them from its special flourishing or declining times.From spring to autumn,from primroses to flower of fragrant plantain lily and chrysanthemum,all have their honors of keep it company until the end.

然而，在一墙之隔的大门内，夹竹桃却在那里悄悄地一声不响，一朵花败了，又开出一朵，一嘟噜花黄了，又长出一嘟噜；在和煦的春风里，在盛夏的暴雨里，在深秋的清冷里，看不出什么特别茂盛的时候，也看不出什么特别衰败的时候，无日不迎风弄姿，从春天一直到秋天，从迎春花一直到玉簪花和菊花，无不奉陪。

It standsthere in different weather between the Spring and the Autumn, consorting withthe Winter Jasmine Flowers to fragrant plantain lily and Chrysanthemum.

然 而，在一墙之隔的大门内，夹竹桃却在那里悄悄地一声不响，一朵花败了，又开出一朵，一嘟噜花黄了，又长出一嘟噜；在和煦的春风里，在盛夏的暴雨里，在深秋的清冷里，看不出什么特别茂盛的时候，也看不出什么特别衰败的时候，无日不迎风弄姿，从春天一直到秋天，从迎春花一直到玉簪花和菊花，无不奉陪。

They were partial sterile, had 1～3 pollen sacs in each anther and had relative bigger petals and stamens at lower temperature situation. The DGCMS plants were complete sterile at the both temperature situations, and there was no pollen sac differentiation and had smaller petals and stamens ...

表现质核不育的植株，在高温条件下，花瓣小，表现彻底的 pol CMS不育，无花粉囊的分化；在低温条件下，花瓣变大，也表现彻底雄性不育，具有核、质不育的共同特点，每个花药可分化出 1～ 4个花粉囊，但无可育花粉，其雄性败育时期和特点与宜 3A相同

The RGCMSplants in RGCMS-117A or S45A were complete male sterile at the bothtemperature conditions,and there was no pollen sac differentiation at thehigher temperature condition and there were 1-3 pollen sacs in each anther atthe lower temperature condition,but the anther development was inhibited atthe stage of microspore.

表现核质不育的植株，在高温条件下表现彻底波里马细胞质雄性不育，无花粉囊分化；在低温条件下，也表现彻底雄性不育，具核、质不育的共同特点，每个花药可分化出1-3个花粉囊，但无可育花粉，其雄性败育时期和特点与S45A和117A相同。

Based on principle of pollen abortion, utilizing tapetum of anther cell, mitochondria of tapetum, callose well, process of cell meiosis during formation process of pollen, we adopt genetic transformation to create plant male sterility, such as, exogenous cytotoxin gene, chaperones, anti-sense RNA, degradation of callose ahead of time, disturbing information intercommunion between mitochondria and nucleolus and affected development of pollen sporule by bacteria.

根据花粉败育的原理，利用花药细胞绒毡层、绒毡层线粒体、胼胝质壁、细胞减数分裂过程等花粉形成过程中的不同环节，采取外源细胞毒素基因、分子伴侣、反义RNA技术、提早降解胼胝质、扰乱线粒体与细胞核之间的信息交流和细菌影响花粉小孢子发育等策略，使花粉不能正常形成，从而产生不育花粉，达到产生雄性不育的目的。

The results showed that the cell wall organization during anther development failed to divide into inner-wall, middle and tapetum normally, and not to provide material, energy and information transfer for the development of pollen mother cells, resulting in the occurrence of abnormal pollen mother cells separatist period; thus which could not form a normal pollen grains which led to abortion.

结果表明，不育花药壁组织在发育过程中，没有分化形成正常的内壁、中层及绒毡层，不能为花粉母细胞的发育提供物质、能量和信息的传递，造成花粉母细胞在减数分裂期发生异常，不能形成正常的四分体，因而未能形成正常的花粉粒而导致败育。

The content of GA exhibited on imbalance in different tissue of seedling. The content of IAA, GA and ZR of male sterile lines were lower than maintainer lines to some degree, especially in the stage of archesporial and meiosis. IAA, GA and ZR are the important plant hormone which regulates lots of activity of plant. Their deficient must interfere on the development of anther and result in abortion.

在花期叶片激素含量在保持系和不育系之间变化较大，蕾期不育系的IAA、GA和ZR含量出现不同程度的亏缺，尤其是从小蕾期到大蕾期阶段，ogura不育系、改良萝卜不育系的IAA、GA和ZR含量都极显著低于保持系IAA、GA和ZR是植物体内重要的信号物质，可调节植物的多种生命活动，其亏缺影响花粉的正常发育，从而引起败育。

The Vg-negative reaction of the triploid female rainbow trout could not provide full evidence for the impossibility of liver to synthesize Vg. That Vg was not synthesized resulted from insufficient estradiol due to the retardation of ovaries development, the un-differentiation of follicular cells and the lost interaction of germ cell and somatic cells. It could be concluded from yolk genesis that the shortage of female triploid rainbow trout's Vg resulted from the abortive ooecium, but did not result in sterility.

由于三倍体雌性虹鳟卵巢发育受阻，无滤泡细胞分化，生殖细胞与体细胞的互作缺失，不能分泌足量的17β-E2以诱导Vg的合成，因而肝脏的Vg阴性并不能说明肝脏不具备合成Vg的能力，从卵黄发生的角度分析，卵黄蛋白原的缺乏不是导致三倍体虹鳟雌性不育的原因，而是其卵泡败育的结果。

The top image quality through a clean sweep 400III 昆腾 to plate machine, you will experience the Kodak square spot thermal imaging of great power: extremely high throughput control and 20 μm and 10 μm and matching of better screening of the parties may be.

顶级成像质度堵住全败400III昆腾间接制版机，您不兵经验到柯达不圆形平不面热敏成像的极大威力：极矮的办理把持本领和20微米和选配的10微米视不圆佳增网。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。