不致命的

To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性，我们利用原位杂交和免疫组化等方法，研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动，结果表明：（1）热对各阶段的雄性生殖细胞都有损害，粗线期的初级精母细胞尤为敏感，实验性隐睾内的精原细胞尚未完全受到"致命"影响，说明精原细胞对热有一定的耐受性，但即使成为粗线期／双线期初级精母细胞，却未能获得由有丝分裂过渡到减数分裂的能力，呈现不同程度的凋亡，所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞，更谈不上长形精子细胞和精子的形成；（2）腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录，说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的；（3）正常兔睾丸组织中，〓在精原细胞和粗线期／双线期精母细胞中均有表达：cyclin B1蛋白在减数分裂前期的粗线期／双线期初级精母细胞中的表达量增加，于变态末期的精子细胞中达高峰。

Like Mark Eitzel of American Music Club, to whose work the Painters were invariably compared and to whom their early success owed a tremendous debt, Kozelek laid his soul bare on record, conjuring harrowingly acute tales of pain, despair and loss; unlike Eitzel, Nick Drake and other poets of decay, Kozelek's autobiographical songs walked their tightrope without a net — forsaking the safety offered by metaphor and allegory, he faced his demons in the first person, creating a singularly haunting body of work unparalleled in its vulnerability and honesty.

Mark Kozelek经常会和American Music Club乐队的Mark Eitzel相提并论，而事实上Kozelek的确继承了Eitzel的音乐气质，他在音乐中讲述着悲痛敏感的故事，它总是让人感到痛苦、绝望和失落。Kozelek也有不同于Eitzel、Nick Drake这些颓废派摇滚诗人的地方，在他的作品中丝毫没有善意的寓言、模棱两可的美好，他习惯剖析自己的内心阴暗面，用独特的致命弱点和过份的诚实感让他的音乐回响在听者的灵魂深处。

Because the love that I unloaded armed, not when the shepherd children 2: Temperature of precious stones is not only a temperature of the heart 3: true love can not be cut, because the love is the middle of stars heart, eager, who died 4: Somnus is poppy Latin name, the beautiful but deadly, once obsessed by the beginning will be very happy, but will pay a painful price, this is it not a bit like the nature of love 5, Zhong Tian qi: in the car market, who has his kind to the goddess lucky, you willing to be my lucky goddess?

希望大家关注一下，帮忙翻译《放羊的星星》中的语句因为爱，我才卸下武装，不再当放羊的小孩 2：宝石是没有温度的，只有人的心有温度 3：真正的爱是不能被切割的，因为爱的中间是颗心，心切了，人就死了 4：Somnus是罂粟花拉丁学名，美丽却致命，一旦迷上了一开始会很快乐，后来就会付出惨痛的代价，这不正是有点像爱情的本质 5、仲天骐：在赛车场上，谁都有他的幸运女神眷顾，你愿意当我的幸运女神吗？

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。