不能获得的

The key is to find an end user to make sure you get value out of it in order to subsidize the cost," Giroux said."You can't make money from the tipping fees and carbon avoidance credits—at least not right now.

Giroux说，该计划的关键是找到足够的终端用户，并从它们那里获得利润来弥补花费，我们不能从垃圾倾倒费用和碳减排补贴赚钱，至少现在还不是时候。

The cold wind does not attack in one's face showing mercy , can't call coldly at this moment , let alone move , according to dogged willpower like this, insist on quietly ," the good beginning is half of success " but succeed, in fact persist attentively.

寒风不留情地迎面扑来，这时不能喊冷，更不能动一动，就这样凭着顽强的毅力，默默坚持着，"好的开头是成功的一半"但要获得成功，其实还要好好地坚持到底。

You only get half way down the dark road and then, seeing something you feel you can't face, you scamper back to the safety of a tender hope.

你只获得一半的黑暗的道路，然后，看到一些你觉得你能不能面对，你疾走回到招标希望的安全。

He even sued, but the Austin District Court issued a curious ruling against Ford in 2000, stating that:"The plaintiff is prohibited from selling motor vehicles to consumers by mail, phone calls, leafleting, skywriting, or drum signals, as well as on a plane, on a train, in a house, or with a mouse."

他甚至向法庭起诉，希望能获得这种权利，但是Austin地方法庭在2000年对福特公司作出了如下的的一道让人觉得奇怪的栽决：原告不能通过电子邮件，电话，传单，飞机尾汽喷字，drum sigals，同时也禁上在飞机上，火车上，住宅以及通过电脑向消费者销售机动车。

Signal peptide sequence was removed lest it couldnt be recognized by Bacillus subtilis, gene coding for mature peptide was ligated to downstream of sacB signal peptide sequence of pWB980 to form a new ORF and generate pWB980-LipA, gene coding for its chaperone was also amplified and ligated to the downstream of LipA to generate a secretion/expression vector pWB980-LipAB.thechaperone gene was sequenced and analyzed by multiple alignments, resulted showed that there were mutations of nucleotides(1～7) and amino acids (1～2) with the other reported genes.

为了防止枯草芽孢杆菌信号肽酶不能识别脂肪酶的信号肽，切除掉脂肪酶的信号肽编码序列，与枯草芽孢杆菌分泌表达质粒pWB980连接并与质粒上的SacB信号肽构成一个完整的开放式阅读框，获得重组质粒pWB980-LipA，因为该脂肪酶的活性表达需要一个特异性分子伴侣帮助折叠成有活性的构象，将脂肪酶分子伴侣基因串连到脂肪酶基因的下游，获得重组分泌表达载体pWB980-LipAB并且测序分析分子伴侣的基因序列和氨基酸序列并与其它报导的序列进行了比对，发现有1到7个碱基的差异和1到2个氨基酸残基的突变。

Each of his first five albums has gone triple platinum, he blitzed the box office with his summer film debut Initial D and unlike most idols, he can actually write his own music.

他前5张专辑都能获得300万的销量，他的首部电影《头文字D》闪电式攻占票房，而且，他不像其它偶像，他能做他自己喜欢的音乐。

He bid me observe it, and I should always find, that the Calamitles of Life were shared among the upper and lower Part of Mankind; but that the middle Station had the fewest Disasters, and was not exposd to so many Vicisitudes as the higher or lower Part of Mankind; nay, they were not subjected to so many Distempers and Uneasiness either of Body or Mind, as those were who, by vicious Living, Luxury and Extravagancies on one Hand, or by hard Labour, Want of Necessaries, and mean or insufficient Diet on the other Hand, bring Distempers upon themselves by the natural Consequences of their Way of Living; That the middle Station of Life was calculated for, all kind of Vertues and all kinds of Enjoyments; that Peace and Plenty were the Hand-maids of a middle Fortune; that Temperance, Moderation, Quietness, Health, Society, all agreeable Diversions, and all desirable Pleasures, were the Blessings attending the middle Station of Life; that this Way Men went silently and smoothly thro the World, and comfortably out of it, not embarassd with the Labours of the Hands or of the Head, not sold to the Life of Slavery for daily Bread, or harrast with perplexd Circumstances, which rob the Soul of Peace, and the Body of Rest; not enragd with the Passion of Envy, or secret burning Lust of Ambition for great things; but in easy Circumstances sliding gently thro the World, and sensibly tasting the Sweets of living, without the bitter, feeling that they are happy, and learning by every Days Experience to know it more sensibly.

他说，我自己可以从下面的事实中认识到，中间地位的生活确实幸福无比；这就是，人人羡慕这种地位，许多帝王都感叹其高贵的出身给他们带来的不幸后果，恨不得自己出生于贫贱与高贵之间的中间阶层。明智的人也证明，中间阶层的人能获得真正的幸福。《圣经》中的智者也曾祈祷：&使我既不贫穷，也不富裕。&他提醒我，只要用心观察，就会发现上层社会和下层社会的人都多灾多难，唯中间阶层灾祸最少。中间阶层的生活，不会像上层社会和下层社会的人那样盛衰荣辱，瞬息万变。而且，中间地位不会像阔佬那样因挥霍无度、腐化堕落而弄得身心俱病；也不会像穷人那样因终日操劳、缺吃少穿而搞得憔悴不堪。唯有中间地位的人可享尽人间的幸福和安乐。中等人常年过着安定富足的生活。适可而止，中庸克己，健康安宁，交友娱乐，以及生活中的种种乐趣，都是中等人的福份。这种生活方式，使人平静安乐，怡然自得地过完一辈子，不受劳心劳力之苦。他们既不必为每日生计劳作，或为窘境所迫，以至伤身烦神；也不会因妒火攻心，或利欲薰心而狂躁不安。中间阶层的人可以平静地度过一生，尽情地体味人生的甜美，没有任何艰难困苦；他们感到幸福，并随着时日的过去，越来越深刻地体会到这种幸福。

No conclusion about the clinical benefit of instrumenting a spinal fusion could be made.

目前尚不能得出器械融合更有益于临床疗效的结论，但有一定量的证据表明器械融合能提高获得稳定融合的机会。

No conclusion about the clinical benefit of instrumenting a spinal fusion could be made. However, there is moderate evidence that the use of instrumentation improves the chance of achieving solid fusion.

目前尚不能得出器械融合更有益于临床疗效的结论，但有一定量的证据表明器械融合能提高获得稳定融合的机会。

Also many propositions from psychology: eg that the powers of the soul are not merely accidents even natural and necessary of the soul, that they are not really distinct from the substance of the soul or from one another; that sense perception is not purely passive; that the intellect can recognize the singular directly, not merely indirectly; that the soul separated from the body forms its knowledge from things themselves, not merely from the ideas which it has acquired through life or which God infuses into it; that the soul is not united with the body for the purpose of acquiring knowledge through the senses but for the purpose of forming with it a new species, ie human nature; that the moral virtues are not necessarily inter se connexae, etc. Also many propositions concerning the doctrine of the angels: eg that the angels can be numerically distinct from one another, and therefore several angels can belong to the same species; that it is not merely through their activity or the application of their powers that angels can be in a given place; that they cannot go from place to place without having to traverse the intermediate space; that they do not acquire all natural knowledge from infused ideas only, but also through contemplation of things themselves; that their will must not necessarily will good or evil, according as it has once decided.

也有许多从心理学的主张：例如，权力的灵魂不只是意外甚至自然和必要的灵魂，他们并没有真正有别於的实质或灵魂从彼此；这个意义上的看法是不是纯粹的被动；该智慧可以识别奇异直接，而不是仅仅间接的灵魂脱离身体的知识形式从自己的事情，而不是仅仅从想法，它已经收购了生命或通过上帝注入到它的灵魂是不联合国与机构的目的是通过获取知识的意识，但目的是形成了一个新的物种，即人性的道德并不一定是相互connexae等也有许多主张有关理论天使：例如，天使数值可以从一个不同的另一种，因此，几个天使可以属於同一物种；，它不仅是通过他们的活动或运用其权力，天使可以在一个特定的地方；，他们不能去地点，而无需导线的中间空间；，他们不会获得所有自然知识，只有注入的想法，而且还通过静观事情本身；，他们将必须不一定会好或邪恶的，因为它显示了一旦决定。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。