不经肠的

Evaluation of IEM103 in rabbit animal model.The evaluation was based on the control-challenge study (IEM101 as control). Rabbits were laparotomized, injecteded 10〓 cfu directly into proximal small bowel, and 28 days later, challenged with 0.25--2ug CT and 10〓-10〓 cfu 4 wild toxic strains with different biotypes and serotypes. Experimental group developed antitoxic and significant vibriocidal immune responses to vaccination, and could protect no less than 2ug CT and 10〓-10〓 cfu all wild strains challenge.

IEM103的免疫原性评价：经兔肠段结扎免疫〓cfu IEM103，7天后在外周血中可检测到外周淋巴细胞分泌性抗CT IgG，血清中可检测到抗CT抗体、杀弧菌抗体，P／N均≥2，且可持续至28天。28天后用0.25-2ug CT毒素，四种毒株滨-43，吴江-2，395，1119〓cfu菌量攻毒，实验组可保护至少2ug CT攻毒，而对照组可保护1ug以下CT攻毒，两组均可保护〓cfu四种毒株攻毒（以肠积液量≥1.0ml／cm为不保护判断标准）。

93 Target gene were detected by Tem-PCR from 75 specimen, they were: Hemophilus influenzae 40, Streptococcus penumoniae 36, Acinetobacter baumannii 10, Pseudomonas aeruginosa 4, Staphylococcus aureus 3, the other 9 kinds of bacterium including Escherichia coli、Klebsiella pneumoniae and Enterobactor cloacae were not detected by Tem-PCR, the positive rate of Tem-PCR was 39.9%(75/188).(3) For the 14 kinds of bacterium designed by Tem-PCR, compared with the culture, the sensitivity、specificity and coincidence of Tem-PCR is 51.0%, 68.0%, 58.3% respectively.

2经Tem-PCR技术扩增后，188例标本在Luminex100多功能悬浮点阵仪中有75例呈阳性，共检测出93株病原菌的靶基因，分别是流感嗜血杆菌40株，肺炎链球菌36株，鲍曼氏不动杆菌10株，铜绿假单胞菌4株，金黄色葡萄球菌3株，另外9种Tem-PCR已设计的细菌包括肺炎克雷伯菌、大肠杆菌、阴沟肠杆菌等均未检出，Tem-PCR的阳性率是39.9%(75/188)。

Because of the absence of a limiting hernial sac, mechanical SBO usually occurs in cases of trans mesenteric hernia (Fig 8), and it is impossible to differentiate closed-loop obstructions caused by herniation through the mesenteric defect from those caused

由于后者没有局限性的疝囊，机械性SBO通常发生在肠系膜疝的病例中（图8），而且不能区分经肠系膜缺损疝导致的闭合性肠梗阻与粘连带下的肠脱垂导致的闭合性肠梗阻。

From 1979 to 1985, there were 16 cases of incomplete rotation of the midgut in our hospital. The diagnosis were suggested by roentgenographic study and verified by surgery, 9 of them had voluvlus.

从民国68年2月至民国74年10月期间，因肠阻塞症状而经外科手术確定为中肠不完全旋转症的病人，共有16位，其中9位病人併发有肠扭结。

The results of in situ hybridization showed that the gene of Mdde was expressed mainly in epidermis of the body wall and fat bodies, and no expression signal was detected in tracheae, intestine and musculature. The gene of Mdde in epidermis expressed both in challenged larva and no-challenged larva, whereas the gene of mdde in fat body expressed only in challenged larva.

原位杂交的结果表明：Mdde基因在家蝇幼虫体内主要表达于表皮和脂肪体，在气管、肠和肌肉组织中没有检测到Mdde基因的表达；表皮中Mdde基因在微生物刺激前后均有表达，而脂肪体中Mdde基因经微生物刺激后才表达，刺激前不表达。

Methods After Candida albicans were inoculated into the specific pathogen free mice, the total amount of Candidas in cecum and the amount of which attached to the mucosal membrane were counted at different interent intervals. The lymphocyte proliferation in Peyer's patch and in lamina proper was shown by BrdU incorporation, meanwhile B cells isotype switch in PP was investigated. IgA plasma cells were shown by immunohistochemical staining. Specific IgA antibodies to Candida albicans were measured with ELISA.

采用无特殊病原菌动物经口喂入白念菌，在不同时相处死后，观察肠内白念菌总数及肠粘膜表面白念菌粘附数量；取肠系膜淋巴结做白念菌选择培养，观察移位体内发生率；采用Brdu体内掺入显示局部粘膜淋巴细胞的增殖情况；流式细胞计数潘伊尔氏结细胞表面IgA阳性率；免疫组化染色后计数固有层中IgA浆细胞数量变化；ELISA法测定肠粘液中特异抗白念菌IgA含量。

Kim Won-time four doctors DAVID home and take Chinese medicine is to send a representative of his platoon to use the same tumor abdominal tumor therapy in the treatment of his case,"the pro-Confucian thing door ** Juan Ba Ji-block" in a record Medical Records:"orchard Liu Ping's wife, a baby such as ladle block, say 18 years, cut off by water, Zhufawucuo, so that people wear in January of four Chung, six under, one Qutan, Second barrels, and it does not matter, like Kwai dishes, like the bad form incorrectly, when Chung Ju-I guess the wood, such as sleep plot scratch disease, and Ping gradually."

3，金元时期的四大医家张子和是中医攻下派的代表人物，他同样运用排瘤疗法治疗腹部肿瘤，在他的医案《儒门事亲＊卷八＊积块》中记载了一个医案：&果园刘子平妻，腹中有块如瓢，十八年矣，经水断绝，诸法无措，戴人令一月之内涌四次，下六次，所去痰约一，二桶，其中不化之物，有如葵菜者，烂鱼肠之状，涌时以木如意揣之，觉病积如刮，渐渐而平&。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。