不粘附的

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling . Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied.

在研究过程中，采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术，系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化，T和B淋巴细胞、T淋巴细胞亚群的动态变化规律，淋巴细胞表面某些粘附分子的变化，淋巴细胞和神经元的坏死与凋亡，雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律，脑组织中围官性细胞浸润的细胞类型。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling. Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied. The key research results were.(1)The average percentage of CD19+cell in blood, bursa and spleen, after 3, 5 days of inoculation, was significantly Phybridization revealed that the number of IL-8 and MIP-1βproducing cells was increased in the infected brain.

在研究过程中，采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术，系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化，T和B淋巴细胞、T淋巴细胞亚群的动态变化规律，淋巴细胞表面某些粘附分子的变化，淋巴细胞和神经元的坏死与凋亡，雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律，脑组织中围官性细胞浸润的细胞类型。

Results:(1) CBC possesses a natural trabecular structure. Its components is similar to the mineral of bone.(2) CBC has great cellur biocompatibility. It can promote cell adhesion, proliferation and secretory while needn t modification.(3) In nude mice, the ectopic osteogenesis of MCCAB was obvious which was better than that of CBC alone and the new bone formation increased in pace with time. In the MCCAB, both intramembranous and cartilaginous osteogeneses were seen but the former dominated.(4) When MCCAB was autotransplanted intramuscularly, the osteogenesis was much better than that of negative control groups.(5) The MCCAB can repair autogenous calvaria defects effectively and the effect was much better than that of CBM alone and blank group. Conclusion:(1) CBC possesses good biocompatibility, osteoconduction and porous structure, can be used as scaffold material for constructing of tissue engineering bone.

结果：(1)CBC具有天然的多孔网状结构，最大程度地保持了骨矿的微结构特征，可以作为骨组织工程的支架材料；(2)CBC细胞亲和性好，不需作修饰即能促进成骨细胞的粘附、增殖和分泌活动；(3)MSO/CBC复合人工骨在裸鼠皮下异位成骨作用明显，兼有膜内成骨和软骨内成骨的特点，成骨效果明显优于单纯CBC植入组；(4)MSO/CBC复合人工骨兔自体肌袋异位成骨作用明显，血管化充分，成骨效果明显优于单纯CBC植入组；(5)MSO/CBC复合人工骨修复兔颅骨临界性缺损效果好，明显优于单纯植入CBC组和空白对第四旱区大学硕士学位论文一照组，可以作为骨移植替代材料用于骨缺损畸形修复。

Pills formed from synthetic fibers of high breaking strength remain on the surface because they are too strong and tough to be abraded away.

由高强度的合成纤维生成的纤维球会一直粘附在织物的表面，这是因为纤维的强度过高以至于不会因摩擦而掉落。

Pills formed from synthetic fibers of high breaking strength remain on the surface because they are too strong and tough to be abraded away.

由高强度的全成纤维生成的纤维球会一直粘附在织物的表面，这是因为纤维的强度过高以至于不会因摩擦而掉落。

Using this model three competence-deficient pneumococci, which was identified by PCR and transformation experiment, were constructed. It is found different strain has different bacterial optimal density for being induced into competence. The optimal density for strains 1, 2 and 22 was 0.1, 0.08and 0.07 respectively. The optimal condition for transformation is c plus y culture at pH8.0. In c plus y culture at pH6.8, which restrained pneumococci forming transformation naturally, pneumococci could be induced to form transformation by CSP.It is concluded that we could induce different S.pneumoniae by CSP into competence in their optimal density in laboratory. 2. It was discussed the relationship between pneumococcal transformation formation and its opportunistic pathogenesis. Comparing their virulence with that of parental strains by challenging mice intraperitoneally and adhering to ECV-304 cell respectively.

结果表明不同肺炎链球菌株的转化最适菌密度不同，1、2和22号菌株的最适转化密度分别为0.1、0.08和0.07；用于转化的CTM培养基最适为pH 8.0的C+Y培养基；在自然转化下不能诱导转化发生的pH 6.8的C+Y培养基中，使用CSP诱导，也能促使细菌发生转化，只是转化效率较低；结论：实验室条件下，不同细菌在一定菌密度时可利用CSP诱导肺炎链球菌成为感受态发生转化。2、为探讨肺炎链球菌转化对毒力表达的影响，本研究分别通过体外粘附人脐静脉血管内皮细胞ECV-304和体内小鼠毒力实验观察1、2和22号肺炎链球菌和其转化缺陷菌株1t、2t和22t毒力的差异。

Or, how does VEC influence these characters and functions of its neighboring TC. These are all unclear. On the other hand, How does TC adhere to and destruct the vascular endothelium to enter and come out of the vascular cavity during the tumor haematogenous metastasis?

反之，位于TC邻近的VEC对TC的生物学特性、功能又如何影响以促进肿瘤血管系统的建立，尚不清楚；另一方面，在肿瘤的血行转移中TC如何粘附、破坏血管内皮以进出血管腔？

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。