不破坏的

A novel double hairpin molecular beacon that has more stable structure than traditional molecular beacon has been designed. Its hairpin structure will not fully open when it hybridizes to cDNA but the hybrid can be cut by restriction endonucleases, which leads to destroy of its structure and restore the fluorescence.

本论文的主要内容包括以下三个方面：(1)基于双发夹分子信标分析核酸甲基化酶及药物筛选我们设计了新型分子信标-双发夹分子信标，该探针具有比传统分子信标更稳定的分子结构，与互补核酸链杂交后发夹结构不完全打开，但在酶切反应后其发夹结构被破坏，荧光又可恢复。

Ali," observed his master, as the Nubian entered the chamber,"you have frequently explained to me how more than commonly skilful you are in throwing the lasso, have you not?

而基督山也不愿再多耽误时间了，那样怕破坏他所希望得到的效果，便鞠了一躬，也告辞了，只剩腾格拉尔一个人去受他妻子的怒骂了。

Really afraid to broken your dream, but whispers of the fall have already peeked through the leaves.

真的不想破坏你的梦，但是秋日的私语已经在叶子上若隐若现。

It is lucky to find them. Otherwise, everybody don't know the buffoons which were the poises of the unadvisable politicians to destroy the public rapport would do any other jackasseries.

幸好让伍泰洋发现了，否则某些被看不清时代主题的政客当作破坏公共关系砝码的苍蝇蚊子还不知要干什么蠢事。

I don't think sabotaging your boss' office is a wise counterattack.

我觉得你肆意破坏上司的办公室算不上是绝妙的反击

These policies peaked during the reign of terror known as the CultureRevolution, from 1966 to 1976, millions were persecuted, children turned downtheir parents, and neighbors snooped on each others, and the states cementedits role as the arbiter that all that was important in life, research of that whetherRevolution destroyed the Chinese family.

在1966年到1976年之间的文化大革命之间，这个政策到达了顶峰，数百万人受到迫害，子女不认父母，邻居互相窥探，百姓生活的方方面面都要经过审查。文化大革命是破坏了中国传统家庭。

All spinning wheels and spindles had been destroyed ,so there was no reminder.

所有的纺织轮子和纱锭已经被破坏，如此在那里不是提醒的人。

Not because of the price, but because the relaxed atmosphere is damaged by these high fences, stiffed ticket windows...

不是门票贵贱的问题，而是那种自由休闲的感觉被那个高高栅栏门给破坏掉了。

It cleans thoroughly without striping your natural protective oil.

它能彻底清洁你的皮肤，又不会破坏表皮本身的自然保护油分。

America cannot peacefully accommodate China's rise because it subverts our role as the world's lone superpower.

美国不'静观容忍'中国的崛起，因为这'将'破坏我们作为世界唯一超级强权的角色。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。