不灭的

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

Our names are the light that glows on the sea waves at night and ...

我们的名字，便是夜里海波上发出的光，痕迹也不留就抿灭了。

The memory of him perishes from the earth, And he has no name on the open plain.

18：17 人对他的记念从地上灭没，他的名字在街上也不存留。

The 3rd And The Mortal 专辑:Tears Laid In Earth I feel this familiar Hunger Cover my mind carry my conscience away My beloved skin is aging Heal me pure Virgin With that lifegiving Blood of yours Salva Me The light of the Moon gives me Strength Col...

爱要怎么说出口词曲：李宗盛教我怎么能不难过你劝我灭了心中的火我还能够怎么说怎么说都是错你对我说离开就会解脱试着自己去生活试着找寻自我别再为爱蹉跎何必为爱蹉跎只是爱要怎么说出口爱要怎么说出口我的心里好难受如果能将你拥有我会。。。

Arrange the patients in a mosquito-free room to be separately treated for not less than 5 days from the date in which the disease was contracted

患者应当在经过彻底灭蚊设备的病房内接受自发病之日算起不少于五日的隔离治疗

And a fluorescence molecule beacon probe is designed between upstream and downstream priers, 5' end of probe can mark fluorescence reporter group FAM, and the 3' end markes fluorescence quenching group DABCYL, and 5' end and 3' end of the probe have 6 bases which can be reflected and complemented to make fluorescence molecule beacon probe do not give out fluorescence signal in free state.

在上下游引物中间设计一条荧光分子信标探针，探针的5'端标记荧光报告基团FAM，3'端标记荧光淬灭基团DABCYL，探针的5'端和3'端有6个碱基能反折互补，使荧光分子信标探针在游离状态下不发出荧光信号。

Our names are the light that glows on the sea waves at night and hen dies without leaving its signature.

我们的名字，便是夜里海波上发出的光，痕迹也不留就抿灭了。

Whose hope shall be cut off, and whose trust shall be a spider's web.

8：13 凡忘记神的人，景况也是这样。不虔敬人的指望要灭没。

The impact of three-stage magic is to eliminate lead to numerous, and ultimately also requested a coach (2 stage to please the coach) and finally passed, when the hunters have a question mark, colorful wizard (Aberdeen Cephalomappa pot experience I do not know the land reclamation no), to the fourth stage of relatively simple.

三阶段的影魔也是导致无数次的灭，最终还请了教练（2阶段也请了教练来），最后终于过了，当时猎人有问号，七彩精灵（肥牛火锅仔我不知道经历了开荒没），到第四阶段就比较简单了。

Civilian in the original stainless steel towers, automatic non-tower water, and 3 tons of plastic cans, multi-purpose section of coal on the basis of±·developed a fairly competitive market, to overcome the concrete, steel, glass and other material for a new type of defect super-large equipment storage. 1-300 tons of wide import modular stainless steel water tank, 3-full-import 30 tons of polyethylene plastic cans; products with anti-acid-base, corrosion-resistance, resist aging, no peculiar smell, not long moss, eliminate sterilization, handsome in appearance, sanitation, any combination of a combination of hot and cold, seismic cracking, corrosion resistance strong, anti-ultraviolet, green environmental protection, impact resistance, the unique advantages such as shortening the construction period; widely used in food, medicine, textile, printing and dyeing, chemical, paper, porcelain , electronics, construction site, yard, distillery, buildings, fire and big hotels, water treatment, pure water plant and other industries.

在原有民用不锈钢水塔、全自动无塔供水器、3吨以下塑胶罐、多功能节煤炉的基础上，厚积薄发，研制出颇具市场竞争力，克服混凝土、钢板、玻璃等材料缺陷的新型供储水超大型设备。1-300吨全进口模块化不锈钢水箱，3-30吨全进口聚乙烯塑胶罐；产品具有抗酸碱、耐腐蚀、拒老化、无异味、不长苔、灭杀菌、造型美观、清洁卫生、任意组合、冷热兼用、抗震防裂、耐蚀性强、抗紫外线、绿色环保、耐受撞击、缩短工期等独特优点；广泛使用于食品、医药、纺织、印染、化工、造纸、瓷厂、电子、工地、料场、酒厂、楼群、消防、大酒店、水处理、纯水厂等行业。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。